Protocols shared by authors via RaP service
Journal
Wound-induced hair neogenesis
Authors: Jenny J. Wei and Thomas H. Leung
Date: Oct 5, 2020，Views：919
Original protocol used in research article：
Activation of TRPA1 nociceptor promotes systemic adult mammalian skin regeneration
Science Immunology, Aug 27, 2020; DOI: 10.1126/sciimmunol.aba5683
Isolation of human hepatocytes by collagenase perfusion
Author: Marti Cabanes-Creus
Date: Oct 5, 2020，Views：1093
Original protocol used in research article：
Restoring the natural tropism of AAV2 vectors for human liver
Science Translational Medicine, Sep 8, 2020; DOI: 10.1126/scitranslmed.aba3312
Drug sensitivity assays
Authors: Ali H Alghamdi and Harry P De Koning
Date: Oct 1, 2020，Views：1032
Original protocol used in research article：
Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416
Lap shear adhesion measurements
Authors: Liming Bian and Xiayi Xu
Date: Oct 1, 2020，Views：904
Original protocol used in research article：
Bioadhesive hydrogels demonstrating pH-independent and ultrafast gelation promote gastric ulcer healing in pigs
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aba8014
Immunohistochemistry (IHC)
Author: Daniel Hasche
Date: Oct 1, 2020，Views：1741
Original protocol used in research article：
Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity
eLife, Aug 4, 2020; DOI: 10.7554/eLife.57626
Ovine model of osteochondral defect repair
Author: Frances Henson
Date: Sep 30, 2020，Views：826
Original protocol used in research article：
Agrin induces long-term osteochondral regeneration by supporting repair morphogenesis
Science Translational Medicine, Sep 1, 2020; DOI: 10.1126/scitranslmed.aax9086
Cryo-EM sample preparation
Authors: Byung-Gil Lee and Jan Löwe
Date: Sep 30, 2020，Views：1206
Original protocol used in research article：
Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3
eLife, Sep 15, 2020; DOI: 10.7554/eLife.59560
Immunoprecipitation and GST pull-down
Author: Arnaud Besson
Date: Sep 29, 2020，Views：1117
Original protocol used in research article：
p27Kip1 promotes invadopodia turnover and invasion through the regulation of the PAK1/Cortactin pathway
eLife, Mar 13, 2017; DOI: 10.7554/eLife.22207
Measurement of heat production (thermogenesis) in cells using ERthermAC dye
Authors: Chih-Hao Wang and Yu-Hua Tseng
Date: Sep 29, 2020，Views：1636
Original protocol used in research article：
CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aaz8664
Heparin-induced filament assembly of tau
Author: Wenjuan Zhang
Date: Sep 28, 2020，Views：1667
Original protocol used in research article：
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584
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