Protocols shared by authors via RaP service
Journal
Protein expression and purification
Authors: Si Liu and Jiangtao Guo
Date: Sep 14, 2020，Views：1182
Original protocol used in research article：
Cryo-EM structures of the human cation-chloride cotransporter KCC1
Science, Oct 25, 2019; DOI: 10.1126/science.aay3129
Human monocyte-derived dendritic cell (moDC) differentiation and suppression of moDCs by Tregs
Authors: Qing Jessica Huang and Megan K. Levings
Date: Sep 13, 2020，Views：1070
Original protocol used in research article：
Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells
Science Translational Medicine, Aug 19, 2020; DOI: 10.1126/scitranslmed.aaz3866
IgH recombination
Author: Masaki Miyazaki
Date: Sep 13, 2020，Views：871
Original protocol used in research article：
The transcription factor E2A activates multiple enhancers that drive Rag expression in developing T and B cells
Science Immunology, Sep 3, 2020; DOI: 10.1126/sciimmunol.abb1455
Synthesis and characterization of CpG-PP, CpG-PM, and CpG-P-ss-M
Authors: Huile Gao and Lin Qin
Date: Sep 10, 2020，Views：947
Original protocol used in research article：
A tumor-to-lymph procedure navigated versatile gel system for combinatorial therapy against tumor recurrence and metastasis
Science Advances, Sep 3, 2020; DOI: 10.1126/sciadv.abb3116
Quantitative RT-PCR analysis
Author: Katherine A Donovan
Date: Sep 10, 2020，Views：991
Original protocol used in research article：
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
eLife, Aug 1, 2018; DOI: 10.7554/eLife.38430
Detection and quantitation of NAD+- and NADH-capped RNA in vivo: hybridization with a radiolabeled oligodeoxyribonucleotide probe
Author: Jeremy G Bird
Date: Sep 10, 2020，Views：935
Original protocol used in research article：
Highly efficient 5' capping of mitochondrial RNA with NAD+ and NADH by yeast and human mitochondrial RNA polymerase
eLife, Dec 12, 2018; DOI: 10.7554/eLife.42179
Histology and molecular phenotyping
Author: Magda Spella
Date: Sep 9, 2020，Views：939
Original protocol used in research article：
Club cells form lung adenocarcinomas and maintain the alveoli of adult mice
eLife, May 29, 2019; DOI: 10.7554/eLife.45571
Sample preparation - Elemental mapping
Author: Gawain McColl
Date: Sep 9, 2020，Views：907
Original protocol used in research article：
Changes in ferrous iron and glutathione promote ferroptosis and frailty in aging Caenorhabditis elegans
eLife, Jul 21, 2020; DOI: 10.7554/eLife.56580
Preparation of brain samples for immunofluorescence, immunoblot, and nucleic acid analysis
Author: Bradlee L. Heckmann
Date: Sep 8, 2020，Views：845
Original protocol used in research article：
Noncanonical function of an autophagy protein prevents spontaneous Alzheimer’s disease
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.abb9036
Thermostability shift assay and library screening
Author: Edmund R.S. Kunji
Date: Sep 7, 2020，Views：926
Original protocol used in research article：
Screening of candidate substrates and coupling ions of transporters by thermostability shift assays
eLife, Oct 15, 2018; DOI: 10.7554/eLife.38821
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