Cell Biology

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    Human Schwann Cells in vitro III. Analytical Methods and a Practical Approach for Quality Control
    Author:  Paula V. Monje, date: 10/05/2023, view: 28, Q&A: 0

    This paper introduces simple analytical methods and bioassays to promptly assess the identity and function of in vitro cultured human Schwann cells (hSCs). A systematic approach is proposed to unequivocally discriminate hSCs from other glial cells,

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    Human Schwann Cells in vitro I. Nerve Tissue Processing, Pre-degeneration, Isolation, and Culturing of Primary Cells
    Authors:  Gabriela I. Aparicio and Paula V. Monje, date: 10/05/2023, view: 176, Q&A: 0

    This paper presents versatile protocols to prepare primary human Schwann cell (hSC) cultures from mature peripheral nervous system tissues, including fascicles from long spinal nerves, nerve roots, and ganglia. This protocol starts with a

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    Isolation of Epithelial and Stromal Cells from Colon Tissues in Homeostasis and Under Inflammatory Conditions

    Inflammation of the gastrointestinal tract is a prevalent pathology in diseases such as inflammatory bowel disease (IBD). Currently, there are no therapies to prevent IBD, and available therapies to treat IBD are often sub-optimal. Thus, an unmet

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    Differentiation of Bone Marrow Monocytes into Alveolar Macrophages-like Cells through Co-culture with Lung Epithelial Cells and Group 2 Innate Lymphoid Cells
    Authors:  Pauline Loos, Thomas Marichal, Bénédicte Machiels and Laurent Gillet, date: 09/20/2023, view: 112, Q&A: 0

    During life, the embryonic alveolar macrophage (AM) population undergoes successive waves of depletion and replenishment in response to infectious and inflammatory episodes. While resident AMs are traditionally described as from embryonic origin,

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    Double Staining with Fluorescent Tracers to Determine Myeloid Cell Migration of Leishmania-infected Cells from Mouse Skin to Lymphatic Tissues by Flow Cytometry
    Authors:  Ashanti C. Uscanga-Palomeque, E. Yaneth Osorio and Peter C. Melby, date: 09/20/2023, view: 214, Q&A: 0

    Immune cell trafficking in steady-state conditions and inflammatory cell recruitment into injured tissues is crucial for the surveillance of the immune system and the maintenance of body homeostasis. Tracking the cell journey from the infection site

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    Functional Phenotyping of Lung Mouse CD4+ T Cells Using Multiparametric Flow Cytometry Analysis
    Authors:  Céline M. Maquet, Laurent Gillet and Bénédicte D. Machiels, date: 09/20/2023, view: 131, Q&A: 0

    Gammaherpesviruses such as Epstein-Barr virus (EBV) are major modulators of the immune responses of their hosts. In the related study (PMID: 35857578), we investigated the role for Ly6Chi monocytes in shaping the function of effector CD4+ T cells in

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    A Large-format Polyacrylamide Gel with Controllable Matrix Mechanics for Mammalian Cell Culture and Conditioned Media Production
    Authors:  Catherine S. Millar-Haskell and Jason P. Gleghorn, date: 09/05/2023, view: 140, Q&A: 0

    Tissue culture plastic has been used for routine cell culture and in vitro experiments for over 50 years. However, cells are mechanically responsive and behave differently on hard surfaces than they do on softer substrates. Polyacrylamide gels have

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    Isolation, Purification, and Culture of Embryonic Melanoblasts from Green Fluorescent Protein–expressing Reporter Mice
    Authors:  Melissa Crawford, Kevin Barr and Lina Dagnino, date: 09/05/2023, view: 118, Q&A: 0

    In this article, we provide a method to isolate embryonic melanoblasts from reporter mouse strains. The mice from which these cells are isolated are bred into the ROSA26mT/mG reporter background, which results in green fluorescent protein (GFP)

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    Endoplasmic Reticulum Isolation: An Optimized Approach into Cells and Mouse Liver Fractionation
    Authors:  Marc Leiro, Raúl Ventura, Nil Rojo-Querol and María Isabel Hernández-Alvarez, date: 09/05/2023, view: 185, Q&A: 0

    The subfractionation of the endoplasmic reticulum (ER) is a widely used technique in cell biology. However, current protocols present limitations such as low yield, the use of large number of dishes, and contamination with other organelles. Here, we

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    Isolation of Embryonic Cardiomyocytes and Cell Proliferation Assay Using Genetically Engineered Reporter Mouse Model
    Authors:  Maren Beall, Deqiang Li and Jihyun Jang, date: 09/05/2023, view: 268, Q&A: 0

    Congenital heart disease (CHD) is often associated with myogenic defects. During heart development, cardiomyocyte growth requires essential cues from extrinsic factors such as insulin-like growth factor 2 (IGF-2). To determine whether and how growth

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