Protocols shared by authors via RaP service
Journal
Immunostaining, hair cell and synaptic counts
Author: Guoqiang Wan
Date: Oct 26, 2020，Views：920
Original protocol used in research article：
Neurotrophin-3 regulates ribbon synapse density in the cochlea and induces synapse regeneration after acoustic trauma
eLife, Oct 20, 2014; DOI: 10.7554/eLife.03564
Assessment of CpG methylation status by bisulfite sequencing
Author: Yijing Su
Date: Oct 23, 2020，Views：876
Original protocol used in research article：
DNA methylation presents distinct binding sites for human transcription factors
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00726
Brain tissue preparation and immunohistochemistry
Authors: Renato Socodato, Teresa Summavielle and João B. Relvas
Date: Oct 23, 2020，Views：931
Original protocol used in research article：
Daily alcohol intake triggers aberrant synaptic pruning leading to synapse loss and anxiety-like behavior
Science Signaling, Sep 21, 2020; DOI: 10.1126/scisignal.aba5754
Measurement of root growth and cell death area in the root tip using Arabidopsis seedlings that grow under DNA stress
Authors: Naoki Takahashi and Masaaki Umeda
Date: Oct 23, 2020，Views：1038
Original protocol used in research article：
A regulatory module controlling stress-induced cell cycle arrest in Arabidopsis
eLife, Apr 4, 2019; DOI: 10.7554/eLife.43944
Fluorescamine-based assay
Authors: Yiwei Yang, Falin Tian, Di Nie, Yuan Liu, Kun Qian, Miaorong Yu, Aohua Wang, Yaqi Zhang, Xinghua Shi and Yong Gan
Date: Oct 22, 2020，Views：822
Original protocol used in research article：
Rapid transport of germ-mimetic nanoparticles with dual conformational polyethylene glycol chains in biological tissues
Science Advances, Feb 6, 2020; DOI: 10.1126/sciadv.aay9937
Dose response curve fitting
Author: Ramnik J Xavier
Date: Oct 21, 2020，Views：974
Original protocol used in research article：
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells
eLife, May 22, 2015; DOI: 10.7554/eLife.05920
Cloning and purification of VapB22, VapC22, and VapBC22
Authors: Krishan Gopal Thakur and Ramandeep Singh
Date: Oct 21, 2020，Views：844
Original protocol used in research article：
VapBC22 toxin-antitoxin system from Mycobacterium tuberculosis is required for pathogenesis and modulation of host immune response
Science Advances, Jun 2, 2020; DOI: 10.1126/sciadv.aba6944
Electrophysiology
Authors: Shangbang Gao and Zhitao Hu
Date: Oct 21, 2020，Views：843
Original protocol used in research article：
Minimum number of synaptic vesicles for the initiation of a single action potential at C. elegans neuromuscular junction
microPublication Biology, Oct 6, 2020; DOI: 10.17912/micropub.biology.000316
Cell preparation for analysis
Author: Alok Kumar
Date: Oct 20, 2020，Views：928
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Quantification of the diversities of V(D)J recombination events for a given CDR3 peptide
Authors: Jian Han and Leonore A Herzenberg
Date: Oct 20, 2020，Views：959
Original protocol used in research article：
Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
eLife, Sep 30, 2015; DOI: 10.7554/eLife.09083
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.