Protocols shared by authors via RaP service
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Assay protocols
Authors: Ali H. Alghamdi and Harry P. De Koning
Date: Nov 5, 2020，Views：1142
Original protocol used in research article：
Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416
Construction of the BiG Mito-Split-GFP strain
Authors: Roza Kucharczyk and Hubert Dominique Becker
Date: Nov 5, 2020，Views：1164
Original protocol used in research article：
Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP
eLife, Jul 13, 2020; DOI: 10.7554/eLife.56649
Single cell library preparation
Author: Christopher A Jackson
Date: Nov 5, 2020，Views：1051
Original protocol used in research article：
Gene regulatory network reconstruction using single-cell RNA sequencing of barcoded genotypes in diverse environments
eLife, Jan 27, 2020; DOI: 10.7554/eLife.51254
Molecular Dynamics simulations
Authors: Alzbeta Tuerkova, Robert Vácha and Ivo Kabelka
Date: Nov 4, 2020，Views：2583
Original protocol used in research article：
Effect of helical kink in antimicrobial peptides on membrane pore formation
eLife, Mar 13, 2020; DOI: 10.7554/eLife.47946
Single-cell RNA sequencing of endosteal bone marrow cells
Author: Leilei Zhong
Date: Nov 3, 2020，Views：1171
Original protocol used in research article：
Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment
eLife, Apr 14, 2020; DOI: 10.7554/eLife.54695
CRISPR-Cas9 sgRNA design and construction
Author: Yen-Lin Huang
Date: Nov 3, 2020，Views：2112
Original protocol used in research article：
Collagen-rich omentum is a premetastatic niche for integrin α2-mediated peritoneal metastasis
eLife, Oct 7, 2020; DOI: 10.7554/eLife.59442
Hfq UV cross-linking, ligation and analysis of hybrids (Hfq-CLASH)
Authors: Stuart William Mckellar, Ira Alexandra Iosub, Ivayla Ivanova and Sander Granneman
Date: Nov 3, 2020，Views：980
Original protocol used in research article：
Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation
eLife, May 1, 2020; DOI: 10.7554/eLife.54655
Generation of Gadl1-null mice
Author: Elaheh Mahootchi
Date: Nov 3, 2020，Views：849
Original protocol used in research article：
GADL1 is a multifunctional decarboxylase with tissue-specific roles in β-alanine and carnosine production
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.abb3713
ssDRIP-seq and DRIP-qPCR
Authors: Yaoyi Li, Wei Xu, Yawei Song, Qin Li, Qianwen Sun and Hongjie Yao
Date: Nov 2, 2020，Views：1340
Original protocol used in research article：
R-loops coordinate with SOX2 in regulating reprogramming to pluripotency
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aba0777
2P-seq
Authors: Yunkun Dang, Yi Liu and Zhipeng Zhou
Date: Nov 2, 2020，Views：959
Original protocol used in research article：
Codon usage biases co-evolve with transcription termination machinery to suppress premature cleavage and polyadenylation
eLife, Mar 16, 2018; DOI: 10.7554/eLife.33569
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