Developmental Biology

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    Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle
    Authors:  Benjamin R. Thomson and Susan E. Quaggin, date: 05/20/2022, view: 45, Q&A: 0

    Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    Authors:  Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk, date: 04/20/2022, view: 961, Q&A: 0

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and

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    Visualization and Purification of Caenorhabditis elegans Germ Granule Proteins Using Proximity Labeling
    Authors:  Hannah L. Hertz, Ian F. Price and Wen Tang, date: 04/20/2022, view: 1044, Q&A: 0

    Membraneless organelles, such as germ granules and stress granules, are liquid-like condensates formed by phase transition. Recently, we and others have adopted proximity-based labeling methods to determine the composition of these membraneless

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    RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros Beetle Trypoxylus dichotomus
    Authors:  Kazuki Sakura, Shinichi Morita and Teruyuki Niimi, date: 04/20/2022, view: 713, Q&A: 0

    In the Japanese rhinoceros beetle Trypoxylus dichotomus, various candidate genes required for a specific phenotype of interest are listed by next-generation sequencing analysis. Their functions were investigated using RNA interference (RNAi) method,

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    Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice
    Authors:  Hiroki Ueharu, Jingwen Yang, Yoshihiro Komatsu and Yuji Mishina, date: 04/05/2022, view: 470, Q&A: 0

    Craniofacial anomalies (CFA) are a diverse group of deformities, which affect the growth of the head and face. Dysregulation of cranial neural crest cell (NCC) migration, proliferation, differentiation, and/or cell fate specification have been

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    Measurement of Contractile Ring Tension Using Two-photon Laser Ablation during Drosophila Cellularization
    Authors:  Swati Sharma and Richa Rikhy, date: 03/20/2022, view: 385, Q&A: 0

    Cytokinesis occurs at the final step of cell division and leads to the separation of daughter cells. It requires assembly and constriction of the actomyosin contractile ring. The phases of assembly and constriction of the contractile ring show an

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    3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines

    The centrosome is the main microtubule-organizing center of animal cells, and is composed of two barrel-shaped microtubule-based centrioles embedded in protein dense pericentriolar material. Compositional and architectural re-organization of the

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    Generation, Maintenance and HBV Infection of Human Liver Organoids
    Authors:  Shahla Romal, Tanvir Hossain and Tokameh Mahmoudi, date: 03/20/2022, view: 928, Q&A: 0

    Hepatitis B virus (HBV) infection represents a major public health problem infecting approximately 400 million people worldwide. Despite the availability of a preventive vaccine and anti-viral therapies, chronic HBV infection remains a major health

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    TUNEL Labeling to Detect Double-stranded DNA Breaks in Caenorhabditis elegans Gonads
    Authors:  Peter A. Kropp, Kyle Rhodehouse and Andy Golden, date: 03/20/2022, view: 420, Q&A: 0

    Analysis of DNA double strand breaks (DSBs) is important for understanding dyshomeostasis within the nucleus, impaired DNA repair mechanisms, and cell death. In the C. elegans germline, DSBs are important indicators of all three above-mentioned

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    Labeling and Tracking Mitochondria with Photoactivation in Drosophila Embryos
    Authors:  Sayali Chowdhary and Richa Rikhy, date: 03/05/2022, view: 1335, Q&A: 0

    Mitochondria are relatively small, fragmented, and abundant in the large embryos of Drosophila, Xenopus and zebrafish. It is essential to study their distribution and dynamics in these embryos to understand the mechanistic role of mitochondrial

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