Developmental Biology


    Isolation of Embryonic Cardiomyocytes and Cell Proliferation Assay Using Genetically Engineered Reporter Mouse Model
    Authors:  Maren Beall, Deqiang Li and Jihyun Jang, date: 09/05/2023, view: 256, Q&A: 0

    Congenital heart disease (CHD) is often associated with myogenic defects. During heart development, cardiomyocyte growth requires essential cues from extrinsic factors such as insulin-like growth factor 2 (IGF-2). To determine whether and how growth

    Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells
    Authors:  Fatma Uzbas and Adam C. O’Neill, date: 09/05/2023, view: 200, Q&A: 0

    The centrosome governs many pan-cellular processes including cell division, migration, and cilium formation. However, very little is known about its cell type-specific protein composition and the sub-organellar domains where these protein

    Stereotactic Delivery of Helper-dependent Adenoviral Viral Vectors at Distinct Developmental Time Points to Perform Age-dependent Molecular Manipulations of the Mouse Calyx of Held
    Authors:  Christian Keine, Mohammed Al-Yaari, Tamara Radulovic and Samuel M. Young Jr., date: 08/20/2023, view: 341, Q&A: 0

    Synapses are specialized structures that enable neuronal communication, which is essential for brain function and development. Alterations in synaptic proteins have been linked to various neurological and neuropsychiatric disorders. Therefore,

    Fabrication of Microfluidic Devices for Continuously Monitoring Yeast Aging
    Authors:  Richard O’Laughlin, Emerald Forrest, Jeff Hasty and Nan Hao, date: 08/05/2023, view: 197, Q&A: 0

    For several decades, aging in Saccharomyces cerevisiae has been studied in hopes of understanding its causes and identifying conserved pathways that also drive aging in multicellular eukaryotes. While the short lifespan and unicellular nature of

    Development of a Mouse Model of Hematopoietic Loss of Y Chromosome
    Authors:  Soichi Sano and Kenneth Walsh, date: 08/05/2023, view: 166, Q&A: 0

    This protocol describes the generation of chimeric mice in which the Y chromosome is deleted from a proportion of blood cells. This model recapitulates the phenomenon of hematopoietic mosaic loss of Y chromosome (mLOY), which is frequently observed

    Fluorescent PCR–based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish
    Authors:  Blake Carrington and Raman Sood, date: 08/05/2023, view: 218, Q&A: 0

    Generation of zebrafish (Danio rerio) models with targeted insertion of epitope tags and point mutations is highly desirable for functional genomics and disease modeling studies. Currently, CRISPR/Cas9-mediated knock-in is the method of choice for

    Three-color dSTORM Imaging and Analysis of Recombination Foci in Mouse Spread Meiotic Nuclei

    During the first meiotic prophase in mouse, repair of SPO11-induced DNA double-strand breaks (DSBs), facilitating homologous chromosome synapsis, is essential to successfully complete the first meiotic cell division. Recombinases RAD51 and DMC1

    Biophysical Analysis of Mechanical Signals in Immotile Cilia of Mouse Embryonic Nodes Using Advanced Microscopic Techniques
    Authors:  Takanobu A. Katoh, Toshihiro Omori, Takuji Ishikawa, Yasushi Okada and Hiroshi Hamada, date: 07/20/2023, view: 600, Q&A: 0

    Immotile cilia of crown cells at the node of mouse embryos are required for sensing leftward fluid flow that gives rise to the breaking of left-right (L-R) symmetry. The flow-sensing mechanism has long remained elusive, mainly because of

    A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues
    Authors:  Mehmet Girgin, Nicolas Broguiere, Lorenzo Mattolini and Matthias Lutolf, date: 07/20/2023, view: 673, Q&A: 0

    Embryonic development is a complex process integrating cell fate decisions and morphogenesis in a spatiotemporally controlled manner. Previous studies with model organisms laid the foundation of our knowledge on post-implantation development;

    Systematic Analysis of Smooth Muscle and Cartilage Ring Formation during Mouse Tracheal Tubulogenesis
    Authors:  Haoyu Wu, Ping Wang, Ziying Liu, Chunyan Lu and Wenguang Yin, date: 07/05/2023, view: 136, Q&A: 0

    The trachea tube is the exclusive route to allow gas exchange between the external environment and the lungs. Recent studies have shown the critical role of mesenchymal cells in tracheal tubulogenesis. Improved methods for studying the dynamics of


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