Protocols shared by authors via RaP service
Journal
On-chip sort, 10X library preparation, and RNA sequencing
Authors: Benjamin W Okaty, Susan M Dymecki and Nikita Sturrock
Date: Oct 19, 2020，Views：1059
Original protocol used in research article：
A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons
eLife, Jun 22, 2020; DOI: 10.7554/eLife.55523
Wireless transmitter and tetrode fabrication
Author: Haleh Fotowat
Date: Oct 19, 2020，Views：866
Original protocol used in research article：
Neural activity in a hippocampus-like region of the teleost pallium is associated with active sensing and navigation
eLife, Apr 3, 2019; DOI: 10.7554/eLife.44119
Antisense oligonucleotide application
Author: Jonathan R Roth
Date: Oct 19, 2020，Views：1075
Original protocol used in research article：
Alzheimer’s disease risk gene BIN1 induces Tau-dependent network hyperexcitability
eLife, Jul 13, 2020; DOI: 10.7554/eLife.57354
Construct design and 3D printing process
Author: Fiona E. Freeman
Date: Oct 19, 2020，Views：1076
Original protocol used in research article：
3D bioprinting spatiotemporally defined patterns of growth factors to tightly control tissue regeneration
Science Advances, Aug 14, 2020; DOI: 10.1126/sciadv.abb5093
CytofRUV vignette
Author: Marie Trussart
Date: Oct 19, 2020，Views：997
Original protocol used in research article：
Removing unwanted variation with CytofRUV to integrate multiple CyTOF datasets
eLife, Sep 7, 2020; DOI: 10.7554/eLife.59630
Skeletal stain followed by micro-computed tomography
Authors: Camilla S Teng, Robert E Maxson and J Gage Crump
Date: Oct 18, 2020，Views：927
Original protocol used in research article：
Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome
eLife, Oct 25, 2018; DOI: 10.7554/eLife.37024
Western blots
Author: Weilin Zhang
Date: Oct 15, 2020，Views：983
Original protocol used in research article：
Hypoxic mitophagy regulates mitochondrial quality and platelet activation and determines severity of I/R heart injury
eLife, Dec 20, 2016; DOI: 10.7554/eLife.21407
RdRp assay for DENV4 NS5Pol
Authors: Cheng-Feng Qin and Zhong-Yu Liu
Date: Oct 15, 2020，Views：975
Original protocol used in research article：
Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization
eLife, Oct 1, 2016; DOI: 10.7554/eLife.17636
Bioinformatics analyses
Authors: Karin Hansson and Daniel Bexell
Date: Oct 15, 2020，Views：788
Original protocol used in research article：
Therapeutic targeting of KSP in preclinical models of high-risk neuroblastoma
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aba4434
Retrograde labeling of motor neurons in adult zebrafish using dextran or DiI
Authors: Buntaro Zempo and Fumihito Ono
Date: Oct 14, 2020，Views：893
Original protocol used in research article：
Synaptic silencing of fast muscle is compensated by rewired innervation of slow muscle
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aax8382
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