Functional Phenotyping of Lung Mouse CD4+ T Cells Using Multiparametric Flow Cytometry Analysis
    Authors:  Céline M. Maquet, Laurent Gillet and Bénédicte D. Machiels, date: 09/20/2023, view: 86, Q&A: 0

    Gammaherpesviruses such as Epstein-Barr virus (EBV) are major modulators of the immune responses of their hosts. In the related study (PMID: 35857578), we investigated the role for Ly6Chi monocytes in shaping the function of effector CD4+ T cells in

    Double Staining with Fluorescent Tracers to Determine Myeloid Cell Migration of Leishmania-infected Cells from Mouse Skin to Lymphatic Tissues by Flow Cytometry
    Authors:  Ashanti C. Uscanga-Palomeque, E. Yaneth Osorio and Peter C. Melby, date: 09/20/2023, view: 162, Q&A: 0

    Immune cell trafficking in steady-state conditions and inflammatory cell recruitment into injured tissues is crucial for the surveillance of the immune system and the maintenance of body homeostasis. Tracking the cell journey from the infection site

    Differentiation of Bone Marrow Monocytes into Alveolar Macrophages-like Cells through Co-culture with Lung Epithelial Cells and Group 2 Innate Lymphoid Cells
    Authors:  Pauline Loos, Thomas Marichal, Bénédicte Machiels and Laurent Gillet, date: 09/20/2023, view: 58, Q&A: 0

    During life, the embryonic alveolar macrophage (AM) population undergoes successive waves of depletion and replenishment in response to infectious and inflammatory episodes. While resident AMs are traditionally described as from embryonic origin,

    Use of Open Surface Plasmon Resonance (OpenSPR) to Characterize the Binding Affinity of Protein–Protein Interactions
    Authors:  Cassie Shu Zhu, Jianhua Li and Haichao Wang, date: 09/05/2023, view: 171, Q&A: 0

    Surface Plasmon Resonance (SPR) is a label-free optical technique to assess protein–protein interaction kinetics and affinities in a real-time setting. Traditionally, Biacore SPR employs a continuous film of gold to detect any change in the

    Determination of Antibody Activity by Platelet Aggregation
    Authors:  Halina H.L. Leung, Jose Perdomo, Zohra Ahmadi and Beng H. Chong, date: 09/05/2023, view: 95, Q&A: 0

    Platelets play an important role in hemostasis by forming clots and stopping bleeding. In immune thrombotic conditions, platelets and leukocytes are aberrantly activated by pathogenic antibodies resulting in platelet aggregates and NETosis, leading

    T Cell Clonal Analysis Using Single-cell RNA Sequencing and Reference Maps
    Authors:  Massimo Andreatta, Paul Gueguen, Nicholas Borcherding and Santiago J. Carmona, date: 08/20/2023, view: 1042, Q&A: 0

    T cells are endowed with T-cell antigen receptors (TCR) that give them the capacity to recognize specific antigens and mount antigen-specific adaptive immune responses. Because TCR sequences are distinct in each naïve T cell, they serve as

    Multi-color Flow Cytometry Protocol to Characterize Myeloid Cells in Mouse Retina Research
    Authors:  Wei Xiao, Rami A. Shahror, Carol A. Morris, Ruth B. Caldwell and Abdelrahman Y. Fouda, date: 08/20/2023, view: 442, Q&A: 0

    Myeloid cells, specifically microglia and macrophages, are activated in retinal diseases and can improve or worsen retinopathy outcomes based on their inflammatory phenotype. However, assessing the myeloid cell response after retinal injury in mice

    VAR2CSA Ectodomain Labeling in Plasmodium falciparum Infected Red Blood Cells and Analysis via Flow Cytometry
    Authors:  Olivia M.S. Carmo and Matthew W.A. Dixon, date: 08/05/2023, view: 176, Q&A: 0

    Presentation of the variant antigen Plasmodium falciparum erythrocyte membrane protein 1 (EMP1) at the surface of infected red blood cells (RBCs) underpins the malaria parasite’s pathogenicity. The transport of EMP1 to the RBC surface is

    Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMS

    The Three-dimensional OrbiTrap Secondary Ion Mass Spectrometry (3D OrbiSIMS) is a secondary ion mass spectrometry instrument, a combination of a Time of Flight (ToF) instrument with an Orbitrap analyzer. The 3D OrbiSIMS technique is a powerful tool

    LiverQuant: An Improved Method for Quantitative Analysis of Liver Pathology
    Authors:  Dominick J. Hellen and Saul Joseph Karpen, date: 07/20/2023, view: 646, Q&A: 0

    Current means to quantify cells, gene expression, and fibrosis of liver histological slides are not standardized in the research community and typically rely upon data acquired from a selection of random regions identified in each slide. As such,


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