Protocols shared by authors via RaP service
Journal
Animal studies
Author: Debomita Chakraborty
Date: Oct 13, 2020，Views：787
Original protocol used in research article：
Fibroblast growth factor receptor 3 activates a network of profibrotic signaling pathways to promote fibrosis in systemic sclerosis
Science Translational Medicine, Sep 29, 2020; DOI: 10.1126/scitranslmed.aaz5506
Whole mount immunofluorescence microscopy of mouse E7.5 embryo for cilia stain
Author: Katsutoshi Mizuno
Date: Oct 12, 2020，Views：1156
Original protocol used in research article：
Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.aba1195
Codon optimized sequence of human ORC genes
Author: Matt J Jaremko
Date: Oct 12, 2020，Views：953
Original protocol used in research article：
The dynamic nature of the human origin recognition complex revealed through five cryoEM structures
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58622
Chemicals and synthetic DNA
Author: Monica Rosas-Lemus
Date: Oct 12, 2020，Views：841
Original protocol used in research article：
High-resolution structures of the SARS-CoV-2 2′-O-methyltransferase reveal strategies for structure-based inhibitor design
Science Signaling, Sep 28, 2020; DOI: 10.1126/scisignal.abe1202
Fractionation of neuronal cells into soma and neurite fractions
Authors: Raeann Goering and J Matthew Taliaferro
Date: Oct 12, 2020，Views：1428
Original protocol used in research article：
FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences
eLife, Jun 8, 2020; DOI: 10.7554/eLife.52621
Lifespan assay
Author: Michael Petrascheck
Date: Oct 12, 2020，Views：940
Original protocol used in research article：
Translation attenuation by minocycline enhances longevity and proteostasis in old post-stress-responsive organisms
eLife, Nov 27, 2018; DOI: 10.7554/eLife.40314
Protein expression for crystal generation
Author: Sean Fanning
Date: Oct 12, 2020，Views：908
Original protocol used in research article：
Estrogen receptor alpha somatic mutations Y537S and D538G confer breast cancer endocrine resistance by stabilizing the activating function-2 binding conformation
eLife, Feb 2, 2016; DOI: 10.7554/eLife.12792
Tumor dissociation into single-cell suspension
Author: Syn Kok Yeo
Date: Oct 11, 2020，Views：4276
Original protocol used in research article：
Single-cell RNA-sequencing reveals distinct patterns of cell state heterogeneity in mouse models of breast cancer
eLife, Aug 25, 2020; DOI: 10.7554/eLife.58810
Isoelectric focusing (IEF)
Author: Steffen Preissler
Date: Oct 11, 2020，Views：1078
Original protocol used in research article：
AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
eLife, Dec 17, 2015; DOI: 10.7554/eLife.12621
Cell lysis
Authors: Jonathan M Budzik and Jeffery S Cox
Date: Oct 6, 2020，Views：1191
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
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