Quantifying Single and Dual Channel Live Imaging Data: Kymograph Analysis of Organelle Motility in Neurons
    Authors:  Laura Digilio, Lloyd P. McMahon, Alois Duston, Chan Choo Yap and Bettina Winckler, date: 05/20/2023, view: 269, Q&A: 0

    Live imaging is commonly used to study dynamic processes in cells. Many labs carrying out live imaging in neurons use kymographs as a tool. Kymographs display time-dependent microscope data (time-lapsed images) in two-dimensional representations

    Implementation of a Drug Screening Platform to Target Gch1 Expression in Injured Mouse Dorsal Root Ganglion Neurons

    Management of neuropathic pain is notoriously difficult; current analgesics, including anti-inflammatory- and opioid-based medications, are generally ineffective and can pose serious side effects. There is a need to uncover non-addictive and safe

    Simultaneous Microendoscopic Calcium Imaging and EEG Recording of Mouse Brain during Sleep
    Authors:  Sasa Teng and Yueqing Peng, date: 05/05/2023, view: 155, Q&A: 0

    Sleep is a conserved biological process in the animal kingdom. Understanding the neural mechanisms underlying sleep state transitions is a fundamental goal of neurobiology, important for the development of new treatments for insomnia and other

    Assessment of Chemosensory Response to Volatile Compounds in Healthy, Aged, and Neurodegenerative Caenorhabditis elegans Models
    Authors:  Cira Crespo and Roberto Grau, date: 05/05/2023, view: 163, Q&A: 0

    A basic function of the nervous system is to confer the ability to detect external stimuli and generate appropriate behavioral and physiological responses. These can be modulated when parallel streams of information are provided to the nervous

    Protocol for 3D Bioprinting Mesenchymal Stem Cell–derived Neural Tissues Using a Fibrin-based Bioink

    Three-dimensional bioprinting utilizes additive manufacturing processes that combine cells and a bioink to create living tissue models that mimic tissues found in vivo. Stem cells can regenerate and differentiate into specialized cell types, making

    Long-term in toto Imaging of Cellular Behavior during Nerve Injury and Regeneration
    Authors:  Weili Tian, Andrés González-Suarez and Hernán López-Schier, date: 05/05/2023, view: 260, Q&A: 0

    Accidental wounding of the peripheral nervous system leads to acute neural dysfunction. Normally, chronic deficits are overcome because peripheral nerves naturally regenerate. However, various genetic and metabolic defects can impair their natural

    A Modified Acyl-RAC Method of Isolating Retinal Palmitoyl Proteome for Subsequent Detection through LC-MS/MS

    Palmitoylation is a unique and reversible posttranslational lipid modification (PTM) that plays a critical role in many cellular events, including protein stability, activity, membrane association, and protein–protein interactions. The dynamic

    Fluorescence Assays for Real-Time Tracking of Cell Surface Protein Internalization and Endosomal Sorting in Axons of Primary Mouse Hippocampal Neurons
    Authors:  Tai Chaiamarit, Yin Wu, Adriaan Verhelle and Sandra E. Encalada, date: 04/05/2023, view: 442, Q&A: 0

    The trafficking and sorting of proteins through the secretory-endolysosomal system is critical for the proper functioning of neurons. Defects in steps of these pathways are associated with neuronal toxicity in various neurodegenerative disorders.

    Isolation of Immunocomplexes from Zebrafish Brain
    Authors:  Jennifer Carlisle Michel and Adam C. Miller, date: 04/05/2023, view: 186, Q&A: 0

    Zebrafish is an excellent model to study vertebrate neurobiology, but its synaptic components that mediate and regulate fast electrical synaptic transmission are largely unidentified. Here, we describe methods to solubilize and immunoprecipitate

    Reconstitution of Membrane-tethered Postsynaptic Density Condensates Using Supported Lipid Bilayer
    Authors:  Zhe Feng and Mingjie Zhang, date: 04/05/2023, view: 117, Q&A: 0

    Eukaryotic cells utilize sub-cellular compartmentalization to restrict reaction components within a defined localization to perform specified biological functions. One way to achieve this is via membrane enclosure; however, many compartments are not


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