Plant Science

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    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids

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    Flow Cytometry-based Measurement of Reactive Oxygen Species in Cyanobacteria
    Authors:  Soumila Mondal and Shailendra P. Singh, date: 05/20/2022, view: 52, Q&A: 0

    Cyanobacteria are Gram-negative oxygen-producing photosynthetic bacteria that are useful in the pharmaceutical and biofuel industries. Monitoring of oxidative stress under fluctuating environmental conditions is important for determining the

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    Quantification of Soil-surface Roots in Seedlings and Mature Rice Plants
    Authors:  Eiko Hanzawa, Yuka Kitomi, Yusaku Uga and Tadashi Sato, date: 05/05/2022, view: 874, Q&A: 0

    Soil-surface roots (SORs) in rice are primary roots that elongate over or near the soil surface. SORs help avoid excessive reduction of stress that occurs in paddy, such as in saline conditions. SORs may also be beneficial for rice growth in

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    Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
    Authors:  Nobuaki Ishihama, Anuphon Laohavisit, Kaori Takizawa and Ken Shirasu, date: 04/20/2022, view: 772, Q&A: 0

    The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed

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    Application of Cadaverine to Inhibit Biotin Biosynthesis in Plants
    Authors:  Nicole M. Gibbs, Shih-Heng Su and Patrick H. Masson, date: 04/20/2022, view: 406, Q&A: 0

    Biotin is an essential vitamin in plants. However, characterization of biotin deficiency has been limited by embryo lethality in mutants, which can only be rescued by supplementation of biotin. Here, we describe a protocol to characterize

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    In vitro Auto- and Substrate-Ubiquitination Assays
    Authors:  Hye Lin Park, Han Yong Lee and Gyeong Mee Yoon, date: 04/05/2022, view: 454, Q&A: 0

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and

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    Activity-based Protein Profiling of Serine Hydrolase Superfamily Enzymes
    Authors:  Achintya Kumar Dolui, Mahadev Latha and Panneerselvam Vijayaraj, date: 03/20/2022, view: 521, Q&A: 0

    Activity-based protein profiling (ABPP) is a chemoproteomics platform to assess the functional state of enzymes in complex biological systems. Over the two decades, ABPP has emerged from a gel-based to gel-free platform, for in-depth proteome

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    A Quick Method to Quantify Iron in Arabidopsis Seedlings
    Authors:  Chandan Kumar Gautam, Huei-Hsuan Tsai and Wolfgang Schmidt, date: 03/05/2022, view: 1132, Q&A: 0

    Iron (Fe) is an indispensable micronutrient for plant growth and development. Since both deficiency, as well as a surplus of Fe, can be detrimental to plant health, plants need to constantly tune uptake rates to maintain an optimum level of Fe.

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    Measuring Endogenous GA and IAA
    Authors:  Yunping Chen, Zhiwu Dan and Shaoqing Li, date: 02/20/2022, view: 1035, Q&A: 0

    Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were analyzed using a liquid chromatography-electrospray ionization-tandem mass spectrometry

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    Fractionation and Extraction of Crude Nuclear Proteins From Arabidopsis Seedlings
    Authors:  Jiajia Zhao, Jinsong Bao and Feifei Xu, date: 01/20/2022, view: 1100, Q&A: 0

    The plant nucleus is an important subcellular organelle that contains the genome, ribosomal RNA, and regulatory proteins, and performs a central role in the functioning and metabolism of the cell. Fractionation of intact nuclei is a crucial process

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