Plant Science


    Fluorescence in situ Localization of Pri-miRNAs in Isolated Arabidopsis thaliana Nuclei

    Here, we present an approach combining fluorescence in situ hybridization (FISH) and immunolabeling for localization of pri-miRNAs in isolated nuclei of A. thaliana. The presented method utilizes specific DNA oligonucleotide probes, modified by

    An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of Chlamydomonas reinhardtii
    Authors:  Nikolai Klena, Giovanni Maltinti, Umut Batman, Gaia Pigino, Paul Guichard and Virginie Hamel, date: 09/05/2023, view: 614, Q&A: 0

    Expansion microscopy is an innovative method that enables super-resolution imaging of biological materials using a simple confocal microscope. The principle of this method relies on the physical isotropic expansion of a biological specimen

    13CO2-labelling and Sampling in Algae for Flux Analysis of Photosynthetic and Central Carbon Metabolism
    Authors:  Or Geffen, David Achaintre and Haim Treves, date: 09/05/2023, view: 121, Q&A: 0

    The flux in photosynthesis can be studied by performing 13CO2 pulse labelling and analysing the temporal labelling kinetics of metabolic intermediates using gas or liquid chromatography linked to mass spectrometry. Metabolic flux analysis (MFA) is

    An Optimized Protocol for Detecting Guard Cell–specific Gene Expression by in situ RT-PCR in Brassica rapa
    Authors:  Yingying Song, Xinlei Guo, Jian Wu, Jianli Liang, Runmao Lin, Zifu Yan and Xiaowu Wang, date: 09/05/2023, view: 105, Q&A: 0

    Since the genetic transformation of Chinese cabbage (Brassica rapa) has not been well developed, in situ RT-PCR is a valuable option for detecting guard cell–specific genes. We reported an optimized protocol of in situ RT-PCR by using a FAMA

    An in vitro Assay to Probe the Formation of Biomolecular Condensates
    Authors:  Yu Zhang and Shen Lisha, date: 09/05/2023, view: 338, Q&A: 0

    Biomolecular condensates are membrane-less assemblies of proteins and nucleic acids formed through liquid–liquid phase separation (LLPS). These assemblies are known to temporally and spatially regulate numerous biological activities and

    A Protocol for Mitotic Metaphase Chromosome Count Using Shoot Meristematic Tissues of Mulberry Tree Species

    Studies on chromosomal status are a fundamental aspect of plant cytogenetics and breeding because changes in number, size, and shape of chromosomes determine plant physiology/performance. Despite its significance, the classical cytogenetic study is

    Improved Methods for Acetocarmine and Haematoxylin Staining to Visualize Chromosomes in the Filamentous Green Alga Zygnema (Charophyta)
    Authors:  Nina Rittmeier and Andreas Holzinger, date: 08/20/2023, view: 149, Q&A: 0

    Genome sizes of Zygnema spp. vary greatly, being unknown whether polyploidization occurred. The exact number of chromosomes in this genus is unknown since counting methods established for higher plants cannot be applied to green algae. The massive

    A Semi-throughput Procedure for Assaying Plant NADP-malate Dehydrogenase Activity Using a Plate Reader
    Authors:  Kevin Baudry and Emmanuelle Issakidis-Bourguet, date: 08/20/2023, view: 177, Q&A: 0

    Chloroplast NADP-dependent malate dehydrogenase (NADP-MDH) is a redox regulated enzyme playing an important role in plant redox homeostasis. Leaf NADP-MDH activation level is considered a proxy for the chloroplast redox status. NADP-MDH enzyme

    Likelihood-based Modeling of Brassiceae Ancient Whole-genome Triplication with POInT
    Authors:  Yue Hao and Gavin C. Conant, date: 08/20/2023, view: 23, Q&A: 0

    Ancient whole-genome duplication and triplication events have a profound impact on present-day plant genomes. However, sub genome assignments in allopolyploids can be technically challenging. The present study used a likelihood-based tool, POInT

    Likelihood Estimation of Arabidopsis Population Parameters with MAPGD
    Authors:  Yue Hao, Matthew S. Ackerman and Michael Lynch, date: 08/20/2023, view: 30, Q&A: 0

    MAPGD ( is a likelihood-based software for population genomics data analysis. Here, we demonstrate a simple tutorial for using MAPGD to analyze Arabidopsis population data. We estimated the allele and genotype


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