Cancer Biology


    Quantification of Chromosomal Aberrations in Mammalian Cells
    Authors:  Inés Paniagua and Jacqueline J. L. Jacobs, date: 08/20/2023, view: 579, Q&A: 0

    Maintenance of genome integrity requires efficient and faithful resolution of DNA breaks and DNA replication obstacles. Dysfunctions in any of the processes orchestrating such resolution can lead to chromosomal instability, which appears as

    Ex vivo Drug Sensitivity Imaging-based Platform for Primary Acute Lymphoblastic Leukemia Cells

    Resistance of acute lymphoblastic leukemia (ALL) cells to chemotherapy, whether present at diagnosis or acquired during treatment, is a major cause of treatment failure. Primary ALL cells are accessible for drug sensitivity testing at the time of

    Isoform-specific, Semi-quantitative Determination of Highly Homologous Protein Levels via CRISPR-Cas9-mediated HiBiT Tagging
    Authors:  Kristina Seiler, Sreoshee Rafiq and Mario P. Tschan, date: 07/20/2023, view: 557, Q&A: 0

    Many protein families consist of multiple highly homologous proteins, whether they are encoded by different genes or originating from the same genomic location. Predominance of certain isoforms has been linked to various pathological conditions,

    mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
    Authors:  Xiaoyu Gao, Yao Li, Guangjun Nie and Xiao Zhao, date: 07/05/2023, view: 361, Q&A: 0

    The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae

    3D Ultrastructural Visualization of Mitosis Fidelity in Human Cells Using Serial Block Face Scanning Electron Microscopy (SBF-SEM)
    Authors:  Nuria Ferrandiz and Stephen J. Royle, date: 07/05/2023, view: 450, Q&A: 0

    Errors in chromosome segregation during mitosis lead to chromosome instability, resulting in an unbalanced number of chromosomes in the daughter cells. Light microscopy has been used extensively to study chromosome missegregation by visualizing

    Large-scale Isolation of Exosomes Derived from NK Cells for Anti-tumor Therapy

    Exosomes are lipid bilayer–enclosed vesicles, actively secreted by cells, containing proteins, lipids, nucleic acids, and other substances with multiple biological functions after entering target cells. Exosomes derived from NK cells have been

    Mitochondrial Replication Assay (MIRA) for Efficient in situ Quantification of Nascent mtDNA and Protein Interactions with Nascent mtDNA (mitoSIRF)
    Authors:  Macy Lozen, Yue Chen, Rebecca A. Boisvert and Katharina Schlacher, date: 05/20/2023, view: 480, Q&A: 0

    Mitochondria play decisive roles in bioenergetics and intracellular communication. These organelles contain a circular mitochondrial DNA (mtDNA) genome that is duplicated within one to two hours by a mitochondrial replisome, independently from the

    Expression and Purification of scFv2H7-P18F3, a Bi-Modular Fusion Protein (BMFP) Targeting Human CD20

    P18F3-based bi-modular fusion proteins (BMFPs), designed to re-direct pre-existing anti-Epstein-Barr virus (EBV) endogenous polyclonal antibodies towards defined target cells, demonstrated efficient biological activity in a mouse tumor model and

    In vivo Drug Screening to Identify Anti-metastatic Drugs in Twist1a-ERT2 Transgenic Zebrafish
    Authors:  Joji Nakayama, Hideki Makinoshima and Zhiyuan Gong, date: 05/20/2023, view: 312, Q&A: 1

    Here, we present an in vivo drug screening protocol using a zebrafish model of metastasis for the identification of anti-metastatic drugs. A tamoxifen-controllable Twist1a-ERT2 transgenic zebrafish line was established to serve as a

    Analysis of RNA Polymerase II Chromatin Binding by Flow Cytometry
    Authors:  Lilli T. E. Bay, Trond Stokke, Randi G. Syljuåsen and Helga B. Landsverk, date: 04/20/2023, view: 625, Q&A: 0

    RNA polymerase II (RNAPII) transcribes DNA into mRNA and thereby plays a critical role in cellular protein production. In addition, RNAPII plays a central role in DNA damage responses. Measurements of RNAPII on chromatin may thus give insight into


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