Protocols shared by authors via RaP service
Journal
Flow cytometry
Authors: Christian Perez-Shibayama, Cristina Gil-Cruz and Burkhard Ludewig
Date: Sep 24, 2020，Views：968
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
Droplet-based scRNA-seq analysis
Authors: Christian Perez-Shibayama, Mechthild Luedge and Burkhard Ludewig
Date: Sep 24, 2020，Views：889
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
Colorimetric RT-LAMP and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Authors: Viet Loan Dao Thi, Michael Knop, Simon Anders, Konrad Herbst, Matthias Meurer and Daniel Kirrmaier
Date: Sep 24, 2020，Views：931
Original protocol used in research article：
A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Science Translational Medicine, Aug 12, 2020; DOI: 10.1126/scitranslmed.abc7075
Untargeted metabolomics
Author: Carlos Guijas
Date: Sep 23, 2020，Views：967
Original protocol used in research article：
Metabolic adaptation to calorie restriction
Science Signaling, Sep 8, 2020; DOI: 10.1126/scisignal.abb2490
Stimulus Ordering
Authors: Michael S Beauchamp and Michael Beauchamp
Date: Sep 23, 2020，Views：940
Original protocol used in research article：
The visual speech head start improves perception and reduces superior temporal cortex responses to auditory speech
eLife, Aug 8, 2019; DOI: 10.7554/eLife.48116
In vitro cytotoxicity assay
Author: Kun Cheng
Date: Sep 22, 2020，Views：894
Original protocol used in research article：
Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
Science Advances, Jul 14, 2020; DOI: 10.1126/sciadv.abb0616
Cellular uptake study
Author: Kun Cheng
Date: Sep 22, 2020，Views：879
Original protocol used in research article：
Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
Science Advances, Jul 14, 2020; DOI: 10.1126/sciadv.abb0616
Dissociation and FACS of embryonic tissue
Authors: Akela Kuwahara and Jeffrey O Bush
Date: Sep 22, 2020，Views：946
Original protocol used in research article：
Delineating the early transcriptional specification of the mammalian trachea and esophagus
eLife, Jun 9, 2020; DOI: 10.7554/eLife.55526
fMRI data analysis software
Author: Chris B Martin
Date: Sep 22, 2020，Views：924
Original protocol used in research article：
Integrative and distinctive coding of visual and conceptual object features in the ventral visual stream
eLife, Feb 2, 2018; DOI: 10.7554/eLife.31873
16S ribosomal RNA gene sequencing
Authors: Bingwei Wang, Lue Dai and Shuo Yang
Date: Sep 21, 2020，Views：853
Original protocol used in research article：
Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation
Science Advances, May 20, 2020; DOI: 10.1126/sciadv.aaz6717
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