Rapid Identification of Pathogens in Severe Pneumonia by Species-specific Bacterial Detector (SSBD)
    Authors:  Cong Zhang, Xiaohui Liang, Yali Qin, Wenkui Yu and Qihan Chen, date: 05/20/2023, view: 198, Q&A: 0

    Fast and accurate detection of pathogenic bacterial infection in patients with severe pneumonia is significant to its treatment. The traditional culture method currently used by most medical institutions relies on a time-consuming culture process

    Novel Antibody-independent Method to Measure Complement Deposition on Bacteria
    Authors:  Toska Wonfor, Shuxian Li and Maisem Laabei, date: 05/05/2023, view: 206, Q&A: 0

    During infection, complement plays a critical role in inflammation, opsonisation, and destruction of microorganisms. This presents a challenge for pathogens such as Staphylococcus aureus to overcome when invading the host. Our current knowledge on

    Development of a Gene Replacement Method for the Filamentous Bacterium Leptothrix cholodnii SP-6
    Authors:  Tatsuki Kunoh, Erika Ono, Tatsuya Yamamoto, Ichiro Suzuki, Minoru Takeda and Nobuhiko Nomura, date: 04/20/2023, view: 285, Q&A: 0

    Genetic strategies such as gene disruption and fluorescent protein tagging largely contribute to understanding the molecular mechanisms of biological functions in bacteria. However, the methods for gene replacement remain underdeveloped for the

    A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria
    Authors:  Nuo Chen and Xiaoming Yuan, date: 04/20/2023, view: 530, Q&A: 0

    In this study, a sonication-based DNA extraction method was developed, in which the whole process can be finished within 10 min. This method is almost zero cost and time-saving, which is useful for high throughput screening, especially in the

    Separating Inner and Outer Membranes of Escherichia coli by EDTA-free Sucrose Gradient Centrifugation
    Authors:  Sheng Shu and Wei Mi, date: 03/20/2023, view: 425, Q&A: 0

    The envelope of Gram-negative bacteria consists of an outer membrane (OM), a peptidoglycan cell wall, and an inner membrane (IM). The OM and IM have different components of proteins and lipids. Separating the IM and OM is a basic biochemical ...

    Protein Pull-down Assay Using HiBiT-tag-dependent Luciferase Activity Measurement
    Authors:  Masashi Arakawa and Eiji Morita, date: 03/20/2023, view: 333, Q&A: 0

    Co-immunoprecipitation or pull-down assays are frequently used to analyze protein–protein interactions. In these experiments, western blotting is commonly used to detect prey proteins. However, sensitivity and quantification problems remain ...

    Molecular Surveillance of Malaria Using the PF AmpliSeq Custom Assay for Plasmodium falciparum Parasites from Dried Blood Spot DNA Isolates from Peru

    Malaria molecular surveillance has great potential to support national malaria control programs (NMCPs), informing policy for its control and elimination. Here, we present a new three-day workflow for targeted resequencing of markers in 13

    Optimized Expression and Isolation of Recombinant Active Secreted Proteases Using Pichia pastoris
    Authors:  Adam Turner, Dylan M. Lanser and Angie Gelli, date: 03/05/2023, view: 366, Q&A: 0

    Recombinant proteins of high quality are crucial starting materials for all downstream applications, but the inherent complexities of proteins and their expression and purification create significant challenges. The Pichia pastoris yeast is a highly

    Sclerotinia sclerotiorum Protoplast Preparation and Transformation
    Authors:  Chi Lan, Lulu Qiao and Dongdong Niu, date: 01/05/2023, view: 453, Q&A: 1

    Sclerotinia sclerotiorum causes white mold, leading to substantial losses on a wide variety of hosts around the world. Many genes encoding effector proteins play important roles in the pathogenesis of S. sclerotiorum. Therefore, establishment of a


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