Stem Cell

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    Optimized CRISPR-Cas9-based Strategy for Complex Gene Targeting in Murine Embryonic Stem Cells for Germline Transmission
    Authors:  Thomas J. O'Neill, Daniel Krappmann and Andreas Gewies, date: 05/20/2022, view: 150, Q&A: 0

    Although CRISPR-Cas9 genome editing can be performed directly in single-cell mouse zygotes, the targeting efficiency for more complex modifications such as the insertion of two loxP sites, multiple mutations in cis, or the precise insertion or

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    Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice
    Authors:  Hiroki Ueharu, Jingwen Yang, Yoshihiro Komatsu and Yuji Mishina, date: 04/05/2022, view: 469, Q&A: 0

    Craniofacial anomalies (CFA) are a diverse group of deformities, which affect the growth of the head and face. Dysregulation of cranial neural crest cell (NCC) migration, proliferation, differentiation, and/or cell fate specification have been

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    Differentiation of Human Induced Pluripotent Stem Cell into Macrophages
    Authors:  Harriet Douthwaite, Aitor Bermejo Arteagabeitia and Subhankar Mukhopadhyay, date: 03/20/2022, view: 848, Q&A: 0

    As a model to interrogate human macrophage biology, macrophages differentiated from human induced pluripotent stem cells (hiPSCs) transcend other existing models by circumventing the variability seen in human monocyte-derived macrophages, whilst

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    Skeletal Stem Cell Isolation from Cranial Suture Mesenchyme and Maintenance of Stemness in Culture
    Authors:  Takamitsu Maruyama, Hsiao-Man Ivy Yu and Wei Hsu, date: 03/05/2022, view: 633, Q&A: 0

    Skeletal stem cells residing in the suture mesenchyme are responsible for calvarial development, homeostatic maintenance, and injury-induced repair. These naïve cells exhibit long-term self-renewal, clonal expansion, and multipotency. They

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    Efficient Method to Differentiate Mouse Embryonic Stem Cells into Macrophages in vitro
    Authors:  Qimin Hai, Juying Han, Sophia Wells and Jonathan D. Smith, date: 02/05/2022, view: 1352, Q&A: 0

    Macrophages are key cells in the innate immune system and play a role in a variety of diseases. However, macrophages are terminally differentiated and difficult to manipulate genetically via transfection or through CRISPR-Cas9 gene editing. To

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    Flow Cytometry Analysis of Planarian Stem Cells Using DNA and Mitochondrial Dyes
    Authors:  Mohamed Mohamed Haroon, Praveen Kumar Vemula and Dasaradhi Palakodeti, date: 01/20/2022, view: 942, Q&A: 0

    Planarians are free-living flatworms that emerged as a crucial model system to understand regeneration and stem cell biology. The ability to purify neoblasts, the adult stem cell population of planaria, through fluorescence-activated cell sorting

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    From 3D to 2D: Harmonization of Protocols for Two-dimensional Cultures on Cell Culture Inserts of Intestinal Organoids from Various Species

    In the expanding field of intestinal organoid research, various protocols for three- and two-dimensional organoid-derived cell cultures exist. Two-dimensional organoid-derived monolayers are used to overcome some limitations of three-dimensional

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    An in vitro Mechanical Damage Model of Isolated Myofibers in a Floating Culture Condition
    Authors:  Yoshifumi Tsuchiya and Yusuke Ono, date: 01/05/2022, view: 1009, Q&A: 0

    Muscle stem cells (satellite cells), located on the surface of myofibers, are rapidly activated from a quiescent state following skeletal muscle injury. Although satellite cell activation is an initial step in muscle regeneration, the stimulation of

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    Live-cell Imaging and Analysis of Germline Stem Cell Mitosis in Caenorhabditis elegans
    Authors:  Réda M. Zellag, Yifan Zhao and Abigail R. Gerhold, date: 01/05/2022, view: 1257, Q&A: 0

    Model organisms offer the opportunity to decipher the dynamic and complex behavior of stem cells in their native environment; however, imaging stem cells in situ remains technically challenging. C. elegans germline stem cells (GSCs) are distinctly

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    Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
    Authors:  Vasavi Nallur Srinivasaraghavan, Faria Zafar and Birgitt Schüle, date: 01/05/2022, view: 524, Q&A: 0

    To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the

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