Protocols shared by authors via RaP service
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Cell culture and calcium switch assay
Authors: Arturo Raya-Sandino and Asma Nusrat
Date: Sep 9, 2026，Views：9
Original protocol used in research article：
Regulation of intestinal epithelial intercellular adhesion and barrier function by desmosomal cadherin desmocollin-2
Molecular Biology of the Cell, Apr 13, 2021; DOI: 10.1091/mbc.E20-12-0775

Stimulated emission depletion microscopy (STED)
Authors: Christina Francisca Vogelaar and Frauke Zipp
Date: Sep 7, 2026，Views：20
Original protocol used in research article：
Interleukin-4 receptor signaling modulates neuronal network activity
The Journal of Experimental Medicine, May 19, 2022; DOI: 10.1084/jem.20211887

Fusion inhibition assay using split GFP
Authors: Sieglinde De Cae and Bert Schepens
Date: Sep 2, 2026，Views：47
Original protocol used in research article：
Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base
Nature Communications, May 30, 2025; DOI: 10.1038/s41467-025-60250-1

Leaf Rolling, Leaf Drying, and Drought Recovery Scores
Authors: Thaura Ghneim, Michael G. Selvaraj and Thaura Ghneim
Date: Sep 1, 2026，Views：52
Original protocol used in research article：
Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage
Frontiers in Plant Science, Jun 12, 2017; DOI: 10.3389/fpls.2017.00994

Lab protocol to quantify siRNA strands via stem loop qPCR
Authors: Stefan Jan-Matthias Rathjen, Eliot Morrison and Marie Wikström Lindholm
Date: Aug 28, 2026，Views：65
Original protocol used in research article：
The beauty of symmetry: siRNA phosphorodithioate modifications reduce stereocomplexity, ease analysis, and can improve in vivo potency
Molecular Therapy. Nucleic Acids, Sep 10, 2024; DOI: 10.1016/j.omtn.2024.102336

HaCaT cell culture
Author: Linda Fahlén Yrlid
Date: Aug 27, 2026，Views：133
Original protocol used in research article：
Bleomycin hydrolase regulates the release of chemokines important for inflammation and wound healing by keratinocytes
Scientific Reports, Dec 31, 2019; DOI: 10.1038/s41598-019-56667-6

Quantification of DNA strand breaks by TUNEL assay in Mycobacterium tuberculosis
Authors: Somnath Shee, Amit Singh and Mayashree Das
Date: Aug 26, 2026，Views：98
Original protocol used in research article：
Biosensor-integrated transposon mutagenesis reveals rv0158 as a coordinator of redox homeostasis in Mycobacterium tuberculosis
eLife, Aug 29, 2023; DOI: 10.7554/eLife.80218

Isolation of microglia and border-associated macrophages
Author: Xiaoting Wu
Date: Aug 19, 2026，Views：155
Original protocol used in research article：
Microglia and CD206+ border-associated mouse macrophages maintain their embryonic origin during Alzheimer’s disease
eLife, Oct 5, 2021; DOI: 10.7554/eLife.71879

Determination of total flavonoid content
Authors: Subramanian Deivanai and Srinivasan Ramachandran
Date: Aug 17, 2026，Views：213
Original protocol used in research article：
EMS-induced mutagenesis in Choy sum (Brassica chinensis var. parachinensis) and selection for low light tolerance using abiotic stress indices
BMC Plant Biology, Nov 21, 2023; DOI: 10.1186/s12870-023-04570-8

Antibody binding and deposition of complement components on bacterial surface
Author: Suzan HM Rooijakkers
Date: Aug 11, 2026，Views：172
Original protocol used in research article：
Promoting Fc-Fc interactions between anti-capsular antibodies provides strong immune protection against Streptococcus pneumoniae
eLife, Mar 22, 2023; DOI: 10.7554/eLife.80669
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