Bio-101 - Improve Research Reproducibility

Tissue histology and immunofluorescence

Authors: Kaylee M Wells and Catherine D McCusker
Date: Feb 10, 2026，Views：5

Original protocol used in research article：

Neural control of growth and size in the axolotl limb regenerate
eLife, Nov 15, 2021; DOI: 10.7554/eLife.68584

2.7. Immunofluorescence Assay to Detect TiLV in the RHTiB Cell Line

Authors: Piyathip Setthawong, Jidapa Yamkasem and Win Surachetpong
Date: Feb 10, 2026，Views：4

Original protocol used in research article：

Development of IgY-Based Passive Immunization Against Tilapia Lake Virus: Development and In Vitro Neutralization Assays
Viruses, Mar 20, 2025; DOI: 10.3390/v17030448

Preparation of acute brain slice for ex vivo time-lapse imaging

Authors: Sunitha Subhramanian and Ilia V Baskakov
Date: Feb 5, 2026，Views：42

Original protocol used in research article：

Dissecting surveying behavior of reactive microglia under chronic neurodegeneration
eLife, Jan 8, 2026; DOI: 10.7554/eLife.107650

Determination of BV2 Microglial Phagocytic Activity (Fluorescent Microspheres / FluoSpheres Assay; Flow Cytometry/Microscopy)

Authors: Marta Matuszewska, Magdalena Cieślik, Anna Wilkaniec and Grzegorz A. Czapski
Date: Feb 5, 2026，Views：30

Original protocol used in research article：

The Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins Protects Against Microglia-Mediated Neuronal Loss In Vitro
Biomolecules, Apr 4, 2025; DOI: 10.3390/biom15040528

NLRP3 oligomerization

Authors: Xiao-Ming Yang and Rong-Hua Yin
Date: Feb 3, 2026，Views：6

Original protocol used in research article：

Pharmacological targeting of NLRP3 deubiquitination for treatment of NLRP3-associated inflammatory diseases
Science Immunology, Apr 29, 2021; DOI: 10.1126/sciimmunol.abe2933

Production and usage of VSV-G pseudotyped lentiviruses

Authors: Soham P. Chowdhury, Diego Acosta-Alvear and Francesca Zappa
Date: Jan 31, 2026，Views：42

Original protocol used in research article：

Baseline unfolded protein response signaling adjusts the timing of the mammalian cell cycle
Molecular Biology of the Cell, May 20, 2024; DOI: 10.1091/mbc.E23-11-0419

Immunofluorescence

Authors: Irene Bolea and Albert Quintana
Date: Jan 28, 2026，Views：61

Original protocol used in research article：

Defined neuronal populations drive fatal phenotype in a mouse model of Leigh syndrome
eLife, Aug 12, 2019; DOI: 10.7554/eLife.47163

3.8. Ethidium Bromide Displacement Fluorescence Assay

Author: Francesco Ruffo
Date: Jan 27, 2026，Views：4

Original protocol used in research article：

Square-Planar vs. Trigonal Bipyramidal Geometry in Pt(II) Complexes Containing Triazole-Based Glucose Ligands as Potential Anticancer Agents
International Journal of Molecular Sciences, Aug 13, 2021; DOI: 10.3390/ijms22168704

Co-immunoprecipitation (co-IP) Following SUMOylation/Ubiquitination Perturbation

Authors: Seung Han Son and Chul Geun Kim
Date: Jan 26, 2026，Views：57

Original protocol used in research article：

SUMOylation-mediated PSME3-20S proteasomal degradation of transcription factor CP2c is crucial for cell cycle progression
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.add4969

Enrichr pathway analysis: Reactome (2022) and Gene Ontology (GO Biological Processes 2023) bulk RNA and luminal proteomics analysis

Authors: Ryan Finnerty and Wipawee Winuthayanon
Date: Jan 23, 2026，Views：59

Original protocol used in research article：

Multi-omics analyses and machine learning prediction of oviductal responses in the presence of gametes and embryos
eLife, Feb 26, 2025; DOI: 10.7554/eLife.100705

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