Protocols shared by authors via RaP service
Journal
Direct co-culture of parasites
Author: Natalia Hernan de Miguel
Date: Dec 11, 2025，Views：0
Original protocol used in research article：
Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication
eLife, May 2, 2023; DOI: 10.7554/eLife.86067
Nanobody screening by phage display
Authors: baolong xia and Norbert Perrimon
Date: Dec 3, 2025，Views：270
Original protocol used in research article：
Phage-displayed synthetic library and screening platform for nanobody discovery
eLife, Aug 1, 2025; DOI: 10.7554/eLife.105887
Synthesis of thanatin and derivatives 1 to 7
Authors: Matthias Schuster, Daniel Obrecht, Mohammed Benghezal, Oliver Zerbe, Gregory Ubert, Emile Brabet and Anatol Luther
Date: Dec 2, 2025，Views：223
Original protocol used in research article：
Peptidomimetic antibiotics disrupt the lipopolysaccharide transport bridge of drug-resistant Enterobacteriaceae
Science Advances, May 24, 2023; DOI: 10.1126/sciadv.adg3683
Maintenance of Drosophila S2 cells and CO2 exposure
Author: Masahiko Shigemura
Date: Nov 24, 2025，Views：412
Original protocol used in research article：
Elevated CO2 regulates the Wnt signaling pathway in mammals, Drosophila melanogaster and Caenorhabditis elegans
Scientific Reports, Dec 3, 2019; DOI: 10.1038/s41598-019-54683-0
In vitro culture of adult mouse DRG neurons
Authors: Pavel Hanč and Ulrich H. von Andrian
Date: Nov 24, 2025，Views：345
Original protocol used in research article：
Multimodal control of dendritic cell functions by nociceptors
Science, Mar 31, 2023; DOI: 10.1126/science.abm5658
Cryopreservation
Author: Jean-Baptiste Raina
Date: Nov 13, 2025，Views：372
Original protocol used in research article：
Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria
eLife, Apr 4, 2017; DOI: 10.7554/eLife.23008
Modeling and computational docking
Authors: Eva Susanne Istvan, Daniel E Goldberg and Elizabeth A Winzeler
Date: Nov 12, 2025，Views：371
Original protocol used in research article：
Cytoplasmic isoleucyl tRNA synthetase as an attractive multistage antimalarial drug target
Science Translational Medicine, Mar 8, 2023; DOI: 10.1126/scitranslmed.adc9249
2.5. Stool Bacterial Extracellular Vesicle Extraction and Removal of Exosomes
Authors: Chanel A. Mosby and Melissa K. Jones
Date: Nov 10, 2025，Views：425
Original protocol used in research article：
Changes in the Murine Microbiome and Bacterial Extracellular Vesicle Production in Response to Antibiotic Treatment and Norovirus Infection
Viruses, Dec 16, 2023; DOI: 10.3390/v15122443
Identification of apoptotic and necrotic cells
Author: H. de Cock
Date: Nov 10, 2025，Views：6
Original protocol used in research article：
Variation of virulence of five Aspergillus fumigatus isolates in four different infection models
PLoS ONE, Jul 9, 2021; DOI: 10.1371/journal.pone.0252948
Histochemical GUS analysis in transgenic rice
Authors: Mitra Mazarei and Charles Neal Stewart Jr.
Date: Nov 7, 2025，Views：382
Original protocol used in research article：
Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass
Biotechnology for Biofuels, Apr 24, 2018; DOI: 10.1186/s13068-018-1119-7
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