Protocols shared by authors via RaP service
Journal
IL-2/JES6-1 and IL-2/S4B6 complexes
Author: Marek Kovar
Date: Dec 19, 2023，Views：2238
Original protocol used in research article：
IL-2/JES6-1 mAb complexes dramatically increase sensitivity to LPS through IFN-γ production by CD25+Foxp3- T cells
eLife, Dec 21, 2021; DOI: 10.7554/eLife.62432
Multiparametric flow cytometry and CATALYST analysis
Authors: María M. Coronel, Haval Shirwan and Andrés J. García
Date: Dec 14, 2023，Views：2114
Original protocol used in research article：
Immunotherapy via PD-L1–presenting biomaterials leads to long-term islet graft survival
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.aba5573
PAS, AB, and AB-PAS staining
Authors: Andong Zhao and Xiaobing Fu
Date: Dec 13, 2023，Views：14
Original protocol used in research article：
Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy
Science Advances, Apr 14, 2021; DOI: 10.1126/sciadv.abb2213
Determination of membrane charge
Author: Andrew M Edwards
Date: Dec 12, 2023，Views：18
Original protocol used in research article：
Colistin kills bacteria by targeting lipopolysaccharide in the cytoplasmic membrane
eLife, Apr 6, 2021; DOI: 10.7554/eLife.65836
Immunofluorescent labelling of adult mouse neuromuscular junctions
Authors: Roberta Sartori, Adam Hagg, Paul Gregorevic and Marco Sandri
Date: Dec 7, 2023，Views：2241
Original protocol used in research article：
Perturbed BMP signaling and denervation promote muscle wasting in cancer cachexia
Science Translational Medicine, Aug 4, 2021; DOI: 10.1126/scitranslmed.aay9592
Mouse Aortic Ring Assay
Authors: Carmen Ruiz de Almodovar and Xiaohong Wang
Date: Dec 5, 2023，Views：2510
Original protocol used in research article：
Protein Phosphatase 2A Mediates YAP Activation in Endothelial Cells Upon VEGF Stimulation and Matrix Stiffness
Frontiers in Cell and Developmental Biology, May 13, 2021; DOI: 10.3389/fcell.2021.675562
Myelin phagocytosis and clearance assays
Author: Rasmus Berglund
Date: Dec 4, 2023，Views：2469
Original protocol used in research article：
Microglial autophagy–associated phagocytosis is essential for recovery from neuroinflammation
Science Immunology, Oct 15, 2020; DOI: 10.1126/sciimmunol.abb5077
TeSLA for TL measurement
Author: Jerry W. Shay
Date: Dec 4, 2023，Views：2266
Original protocol used in research article：
A method for measuring the distribution of the shortest telomeres in cells and tissues
Nature Communications, Nov 7, 2017; DOI: 10.1038/s41467-017-01291-z
CRISPR/Cas9-mediated gene editing
Authors: Alyssa N. Werner and Pascale G. Charest
Date: Nov 29, 2023，Views：2526
Original protocol used in research article：
CRISPR-mediated reversion of oncogenic KRAS mutation results in increased proliferation and reveals independent roles of Ras and mTORC2 in the migration of A549 lung cancer cells
Molecular Biology of the Cell, Sep 20, 2023; DOI: 10.1091/mbc.E23-05-0152
Plasmid map (pGD009)
Authors: Guillaume Diss and Ben Lehner
Date: Nov 27, 2023，Views：5
Original protocol used in research article：
The genetic landscape of a physical interaction
eLife, Apr 11, 2018; DOI: 10.7554/eLife.32472
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.