Protocols shared by authors via RaP service
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Ex vivo larval hemocyte phagocytosis assays
Authors: Mark Austin Hanson, Bruno Lemaitre and Prince Kumar Sah
Date: Jul 17, 2026，Views：214
Original protocol used in research article：
Layers of immunity: Deconstructing the Drosophila effector response
eLife, Nov 5, 2025; DOI: 10.7554/eLife.107030

Determination of CuZn-Superoxide Dismutase (SOD-1) Activity in Human Erythrocytes
Authors: Marta Sylwia Budkowska, Elżbieta Cecerska-Heryć, Zuzanna Marcinowska , Aldona Siennicka and Barbara Dołęgowska
Date: Jul 10, 2026，Views：263
Original protocol used in research article：
The Influence of Circadian Rhythm on the Activity of Oxidative Stress Enzymes
International Journal of Molecular Sciences, Nov 17, 2022; DOI: 10.3390/ijms232214275

Stimulus Videos
Author: Christian Merkel
Date: Jul 7, 2026，Views：244
Original protocol used in research article：
Serial attentional resource allocation during parallel feature value tracking
eLife, Dec 15, 2023; DOI: 10.7554/eLife.91183

Protocol for Detection of Palmitoylated Proteins
Authors: Shuonan Wang and Yuwei Huang
Date: Jul 6, 2026，Views：276
Original protocol used in research article：
Palmitoylation of Tspan4 is essential for migrasome formation
Journal of Cell Biology, Apr 15, 2026; DOI: 10.1083/jcb.202507023

3.7. Encapsulation Efficiency Determination
Author: Lubomír Lapčík
Date: Jul 3, 2026，Views：430
Original protocol used in research article：
Study of Natural Dyes’ Liposomal Encapsulation in Food Dispersion Model Systems via High-Pressure Homogenization
Molecules, Apr 20, 2025; DOI: 10.3390/molecules30081845

BiFC assay for MoEnd3-MoArk1 interaction
Authors: Xiao Li and Zhengguang Zhang
Date: Jun 24, 2026，Views：10
Original protocol used in research article：
MoEnd3 regulates appressorium formation and virulence through mediating endocytosis in rice blast fungus Magnaporthe oryzae
PLoS Pathogens, Jun 19, 2017; DOI: 10.1371/journal.ppat.1006449

Single-cell RT-PCR(nest PCR)
Authors: Yi-jun Zhu and Hua-tai Xu
Date: Jun 24, 2026，Views：261
Original protocol used in research article：
Combinatorial expression of γ-protocadherins regulates synaptic connectivity in the mouse neocortex
eLife, Mar 12, 2024; DOI: 10.7554/eLife.89532

2.4. RNA Extraction From PBMCs, cDNA Reverse Transcription, and Quantification Through Real‐Time qPCR
Authors: Fiona Limanaqi and Evelyn Ferri
Date: Jun 18, 2026，Views：244
Original protocol used in research article：
Effects of the VIVIFRAIL Exercise Protocol on Circulatory and Intracellular Peripheral Mediators Bridging Mitochondrial Dynamics and Inflammation in Robust and Frail Older People
Aging Cell, Mar 4, 2025; DOI: 10.1111/acel.70029

In vitro culture of algae
Authors: Mayuko Hamada and Thomas CG Bosch
Date: Jun 17, 2026，Views：228
Original protocol used in research article：
Metabolic co-dependence drives the evolutionarily ancient Hydra–Chlorella symbiosis
eLife, May 31, 2018; DOI: 10.7554/eLife.35122

tRNA charging measurements
Authors: Ricardo A Cordova, Roberto Pili, Ronald C Wek, Kirk Alan Staschke and Jagannath Misra
Date: Jun 10, 2026，Views：268
Original protocol used in research article：
GCN2 eIF2 kinase promotes prostate cancer by maintaining amino acid homeostasis
eLife, Sep 15, 2022; DOI: 10.7554/eLife.81083
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