Protocols shared by authors via RaP service
Journal
Immunostaining protocol of human 3D bioengineered skeletal muscle whole mounts and cross section for confocal microscopy
Authors: Arne D Hofemeier and Timo Betz
Date: Mar 26, 2024，Views：656
Original protocol used in research article：
Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
eLife, Jan 18, 2021; DOI: 10.7554/eLife.60145
Adult rat DRG neuron culture
Authors: Dong Wang, Susu Mao and Bin Yu
Date: Mar 25, 2024，Views：679
Original protocol used in research article：
Promoting axon regeneration by inhibiting RNA N6-methyladenosine demethylase ALKBH5
eLife, Aug 3, 2023; DOI: 10.7554/eLife.85309
Recombinant expression and purification of human EP300-KIX87 domain from Escherichia coli
Authors: John H. Bushweller, Kwon-Sik Park and Ashish Kabra
Date: Mar 21, 2024，Views：729
Original protocol used in research article：
KIX domain determines a selective tumor-promoting role for EP300 and its vulnerability in small cell lung cancer
Science Advances, Feb 16, 2022; DOI: 10.1126/sciadv.abl4618
Construct design
Authors: Arpita Bharadwaj and Isabelle Baconguis
Date: Mar 20, 2024，Views：753
Original protocol used in research article：
Structure of the human epithelial sodium channel by cryo-electron microscopy
eLife, Sep 25, 2018; DOI: 10.7554/eLife.39340
Reporter design and virus production
Authors: Natalya N Pavlova and Craig B Thompson
Date: Mar 20, 2024，Views：757
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
Protein constructs, expression, and purification
Authors: Alexei Gorelik and Bhushan Nagar
Date: Mar 19, 2024，Views：752
Original protocol used in research article：
Structure of the immunoregulatory sialidase NEU1
Science Advances, May 19, 2023; DOI: 10.1126/sciadv.adf8169
UMAP and ICI analysis
Authors: Bruno Guillotin and Kenneth D. Birnbaum
Date: Mar 19, 2024，Views：10
Original protocol used in research article：
Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327
Community network analysis
Authors: Francesco Colizzi and Modesto Orozco
Date: Mar 18, 2024，Views：5
Original protocol used in research article：
Probing allosteric regulations with coevolution-driven molecular simulations
Science Advances, Sep 8, 2021; DOI: 10.1126/sciadv.abj0786
Small RNA preparation and qPCR
Authors: Luxi Chen and Anming Meng
Date: Mar 18, 2024，Views：749
Original protocol used in research article：
5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
Science Advances, Nov 19, 2021; DOI: 10.1126/sciadv.abh0494
Purification of S. aureus ClpC and ClpP
Authors: Marta Carroni, Bernd Bukau and Axel Mogk
Date: Mar 17, 2024，Views：818
Original protocol used in research article：
Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control
eLife, Nov 22, 2017; DOI: 10.7554/eLife.30120
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.