Protocols shared by authors via RaP service
Journal
Liposome reconstitution
Authors: David N Nathan Tippett and Raimund Dutzler
Date: Aug 26, 2025，Views：192
Original protocol used in research article：
Structural and functional properties of the transporter SLC26A6 reveal mechanism of coupled anion exchange
eLife, Jun 23, 2023; DOI: 10.7554/eLife.87178
Identification of putative enhancers
Authors: Xiaotong Wu and Wei Xie
Date: Aug 22, 2025，Views：2
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Microinjection into mid-gestation E8.5 embryos
Author: Qiang Huang
Date: Aug 14, 2025，Views：5
Original protocol used in research article：
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI: 10.1126/science.aba0210
Preparation of retinol, RBP, and TTR
Author: Regina M Murphy
Date: Aug 14, 2025，Views：35
Original protocol used in research article：
An in vitro model for vitamin A transport across the human blood–brain barrier
eLife, Nov 7, 2023; DOI: 10.7554/eLife.87863
Fluorescent BODIPY-FL vancomycin staining
Authors: Moagi Tube Shaku and Bavesh D Kana
Date: Aug 8, 2025，Views：145
Original protocol used in research article：
A modified BCG with depletion of enzymes associated with peptidoglycan amidation induces enhanced protection against tuberculosis in mice
eLife, Apr 19, 2024; DOI: 10.7554/eLife.89157
Detection of CMV pp65-specific T cells by intracellular staining with anti-INF-γ-PE
Author: David Klatzmann
Date: Aug 1, 2025，Views：158
Original protocol used in research article：
Human thymopoiesis produces polyspecific CD8+ α/β T cells responding to multiple viral antigens
eLife, Mar 30, 2023; DOI: 10.7554/eLife.81274
EdU immunostaining to detect dividing cells
Author: Richard W Naylor
Date: Jul 29, 2025，Views：179
Original protocol used in research article：
A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion
eLife, Nov 5, 2018; DOI: 10.7554/eLife.38911
Expression and purification protocol of ASK1 (residues 88-973, consisting of TBD, CRR, and KD)
Authors: Karolina Honzejkova, Veronika Obsilova and Tomas Obsil
Date: Jul 28, 2025，Views：179
Original protocol used in research article：
The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
eLife, Mar 27, 2024; DOI: 10.7554/eLife.95199
Protocol for GST-Tagged Protein Purification
Authors: Donghai Wang, Hudan Liu, Kaiwei Liang and Guoliang Qing
Date: Jul 25, 2025，Views：99
Original protocol used in research article：
The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastoma
Science Advances, Mar 29, 2023; DOI: 10.1126/sciadv.adf0005
Total nucleated cells preparation enriching HSPC and S100A12high Neu2 for scRNA-seq
Authors: Yingying Huo, Linjie Wu, Erlie Jiang, Sha Hao and Tao Cheng
Date: Jul 14, 2025，Views：177
Original protocol used in research article：
Single-cell dissection of human hematopoietic reconstitution after allogeneic hematopoietic stem cell transplantation
Science Immunology, Mar 17, 2023; DOI: 10.1126/sciimmunol.abn6429
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