Protocols shared by authors via RaP service
Journal
Determination of site‐specific m6A modification levels using SELECT
Author: Tian‐Liang Xia
Date: Nov 4, 2023，Views：1808
Original protocol used in research article：
N(6)‐methyladenosine‐binding protein YTHDF1 suppresses EBV replication and promotes EBV RNA decay
EMBO Reports, Feb 19, 2021; DOI: 10.15252/embr.202050128
Pharyngeal Pumping Assay (Drosophila melanogaster)
Authors: John R Carlson and Ryan M Joseph
Date: Nov 3, 2023，Views：1572
Original protocol used in research article：
A receptor and neuron that activate a circuit limiting sucrose consumption
eLife, Mar 23, 2017; DOI: 10.7554/eLife.24992
2.4. Amoeba–Bacteria Co-Culture
Authors: Mohamed Shaheen and Nicholas J. Ashbolt
Date: Nov 2, 2023，Views：1524
Original protocol used in research article：
Differential Bacterial Predation by Free-Living Amoebae May Result in Blooms of Legionella in Drinking Water Systems
Microorganisms, Jan 15, 2021; DOI: 10.3390/microorganisms9010174
Isolation and characterization of mEVs and EV-depleted supernatant
Authors: Lingjun Tong, Jiong-Wei Wang and Sitong Zhang
Date: Oct 26, 2023，Views：1078
Original protocol used in research article：
Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041
Protein extraction and Macf1 identification by western blot
Authors: Alireza Ghasemizadeh and Vincent Gache
Date: Oct 26, 2023，Views：1050
Original protocol used in research article：
MACF1 controls skeletal muscle function through the microtubule-dependent localization of extra-synaptic myonuclei and mitochondria biogenesis
eLife, Aug 27, 2021; DOI: 10.7554/eLife.70490
Conjugate formation and processing for ultrastructural studies
Authors: Jane C. Stinchcombe and Gillian M. Griffiths
Date: Oct 25, 2023，Views：715
Original protocol used in research article：
Ectocytosis renders T cell receptor signaling self-limiting at the immune synapse
Science, May 25, 2023; DOI: 10.1126/science.abp8933
KIF22 Immunofluorescence
Author: Jason Stumpff
Date: Oct 22, 2023，Views：754
Original protocol used in research article：
Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation
eLife, ; DOI: 10.7554/eLife.78653
Isolation of human bone marrow non-hematopoietic cells for single-cell RNA sequencing
Authors: Hongzhe Li, Sandro Bräunig and Stefan Scheding
Date: Oct 19, 2023，Views：752
Original protocol used in research article：
Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing
eLife, Mar 6, 2023; DOI: 10.7554/eLife.81656
Lentiviral transductions and engineering of stable cell lines
Authors: Kok-Siong Chen and Khalid Shah
Date: Oct 19, 2023，Views：685
Original protocol used in research article：
Bifunctional cancer cell–based vaccine concomitantly drives direct tumor killing and antitumor immunity
Science Translational Medicine, Jan 4, 2023; DOI: 10.1126/scitranslmed.abo4778
Electroporation of CD34+ cells
Authors: Kumarasamypet M Mohankumar and Nithin Sam R
Date: Oct 19, 2023，Views：608
Original protocol used in research article：
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin
eLife, Feb 11, 2022; DOI: 10.7554/eLife.65421
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