Protocols shared by authors via RaP service
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Acid urea polyacrylamide gel electrophoresis (AUPAGE) and Northern analysis
Authors: Sunita Maharjan, Isao Masuda and Ya-Ming Hou
Date: Feb 4, 2025，Views：905
Original protocol used in research article：
Loss of N1-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression
eLife, Aug 12, 2021; DOI: 10.7554/eLife.70619
Measurement of the fluorescence intensity of TCSn:GFP in the Arabidopsis root epidermis
Authors: Naoki Takahashi and Masaaki Umeda
Date: Feb 2, 2025，Views：63
Original protocol used in research article：
Alterations in hormonal signals spatially coordinate distinct responses to DNA double-strand breaks in Arabidopsis roots
Science Advances, Jun 15, 2021; DOI: 10.1126/sciadv.abg0993
Molecular dynamics simulations
Authors: Anja Henning , Serdal Kirmizialtin and Dave Thirumalai
Date: Jan 30, 2025，Views：371
Original protocol used in research article：
Differences in ion-RNA binding modes due to charge density variations explain the stability of RNA in monovalent salts
Science Advances, Jul 20, 2022; DOI: 10.1126/sciadv.abo1190
Protein expression with unnatural amino acids in Pseudomonas aeruginosa
Authors: Eden Ozer and Lital Alfonta
Date: Jan 16, 2025，Views：386
Original protocol used in research article：
An inside look at a biofilm: Pseudomonas aeruginosa flagella biotracking
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg8581
Isolation of sweat glands from mice
Authors: Makiko Kashio and Makoto Tominaga
Date: Jan 16, 2025，Views：357
Original protocol used in research article：
Involvement of TRPV4 in temperature-dependent perspiration in mice
eLife, Jul 4, 2024; DOI: 10.7554/eLife.92993
The preparation of lung organoid-derived monolayers
Authors: Courtney Tindle and Pradipta Ghosh
Date: Jan 13, 2025，Views：350
Original protocol used in research article：
Adult stem cell-derived complete lung organoid models emulate lung disease in COVID-19
eLife, Aug 13, 2021; DOI: 10.7554/eLife.66417
FRET assay
Authors: André Veillette, Bin Li and Zhenghai Tang
Date: Jan 10, 2025，Views：317
Original protocol used in research article：
Cis interactions between CD2 and its ligands on T cells are required for T cell activation
Science Immunology, Aug 5, 2022; DOI: 10.1126/sciimmunol.abn6373
Alexander staining of Arabidopsis anthers
Authors: Upendo Lupanga and Karin Schumacher
Date: Jan 7, 2025，Views：626
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
Identification of the bcd1-2 allele
Author: Jacek Gaertig
Date: Jan 5, 2025，Views：308
Original protocol used in research article：
The Tetrahymena bcd1 mutant implicates endosome trafficking in ciliate, cortical pattern formation
Molecular Biology of the Cell, Jun 20, 2023; DOI: 10.1091/mbc.E22-11-0501
Liver Cholesterol and Triglycerides Measurements
Authors: Shunxing Rong and Jay D. Horton
Date: Dec 31, 2024，Views：308
Original protocol used in research article：
Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice
eLife, Feb 28, 2017; DOI: 10.7554/eLife.25015
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