Protocols shared by authors via RaP service
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In vivo light sheet imaging
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：46
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
Fluorescence-based ion transport assays using Proteoliposomes
Authors: Cristina Manatschal and Karthik Ramanadane
Date: Sep 20, 2025，Views：417
Original protocol used in research article：
Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family
eLife, Jan 10, 2022; DOI: 10.7554/eLife.74589
Mounting
Authors: John Darby Cole and Sebastian Jessberger
Date: Sep 18, 2025，Views：214
Original protocol used in research article：
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging
eLife, Jan 28, 2022; DOI: 10.7554/eLife.68000
Tracer injections
Authors: Natasha O'Brown, Sean G Megason and Chenghua Gu
Date: Sep 12, 2025，Views：228
Original protocol used in research article：
Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326
Principle component analysis (PCA)
Authors: Kingsley Wu, Ta I Hung and Chia-en A Chang
Date: Sep 11, 2025，Views：254
Original protocol used in research article：
PROTAC-induced protein structural dynamics in targeted protein degradation
eLife, Feb 27, 2025; DOI: 10.7554/eLife.101127
ChIP-seq analysis
Authors: Liang Xu, Ye Chen and Tuan Zea Tan
Date: Sep 9, 2025，Views：174
Original protocol used in research article：
Topography of transcriptionally active chromatin in glioblastoma
Science Advances, Apr 30, 2021; DOI: 10.1126/sciadv.abd4676
Liposome reconstitution
Authors: David N Nathan Tippett and Raimund Dutzler
Date: Aug 26, 2025，Views：289
Original protocol used in research article：
Structural and functional properties of the transporter SLC26A6 reveal mechanism of coupled anion exchange
eLife, Jun 23, 2023; DOI: 10.7554/eLife.87178
Identification of putative enhancers
Authors: Xiaotong Wu and Wei Xie
Date: Aug 22, 2025，Views：7
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Microinjection into mid-gestation E8.5 embryos
Author: Qiang Huang
Date: Aug 14, 2025，Views：8
Original protocol used in research article：
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI: 10.1126/science.aba0210
Preparation of retinol, RBP, and TTR
Author: Regina M Murphy
Date: Aug 14, 2025，Views：72
Original protocol used in research article：
An in vitro model for vitamin A transport across the human blood–brain barrier
eLife, Nov 7, 2023; DOI: 10.7554/eLife.87863
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