Protocols shared by authors via RaP service
Journal

BrdU-seq
Authors: Marcel Werner and Stephan Hamperl
Date: Jun 9, 2026，Views：182
Original protocol used in research article：
Transcription-replication conflicts drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery
Nucleic Acids Research, Feb 20, 2025; DOI: 10.1093/nar/gkaf109

Cell migration assay
Authors: Yu-Chia Chen and Aussie Suzuki
Date: Jun 9, 2026，Views：210
Original protocol used in research article：
Inhibition of p38-MK2 pathway enhances the efficacy of microtubule inhibitors in breast cancer cells
eLife, May 29, 2025; DOI: 10.7554/eLife.104859

Immunoprecipitation and Oligo(dT) pulldown
Authors: Brian Andrew Maxwell and J. Paul Taylor
Date: Jun 1, 2026，Views：197
Original protocol used in research article：
Ubiquitination is essential for recovery of cellular activities after heat shock
Science, Jun 24, 2021; DOI: 10.1126/science.abc3593

Screening of protein kinase candidates in silico and in vitro
Author: Anja C Nagel
Date: May 28, 2026，Views：301
Original protocol used in research article：
Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in Drosophila
eLife, Nov 6, 2024; DOI: 10.7554/eLife.89582

Pull-down assays with purified proteins in vitro
Authors: Chi-Chun Yang and Hisao Masai
Date: May 26, 2026，Views：249
Original protocol used in research article：
Claspin is Required for Growth Recovery from Serum Starvation through Regulating the PI3K-PDK1-mTOR Pathway in Mammalian Cells
Molecular and Cellular Biology, Jan 31, 2023; DOI: 10.1080/10985549.2022.2160598

The CGMD simulation
Author: Chun-Lai Ren
Date: May 22, 2026，Views：33
Original protocol used in research article：
Uncovering the molecular mechanism for dual effect of ATP on phase separation in FUS solution
Science Advances, Sep 14, 2022; DOI: 10.1126/sciadv.abo7885

β-Glucuronidase Activities of Feces
Author: Ah-Mee Park
Date: May 15, 2026，Views：263
Original protocol used in research article：
Galectin-3 as a Therapeutic Target for NSAID-Induced Intestinal Ulcers
Frontiers in Immunology, Sep 23, 2020; DOI: 10.3389/fimmu.2020.550366

Preparation of ZG and ZH
Authors: Xiangli Zhang, Zhilong Wang, Hong Jiang, Huajing Zeng, Nan An, Bin Liu, Luyi Sun and Zengjie Fan
Date: May 12, 2026，Views：33
Original protocol used in research article：
Self-powered enzyme-linked microneedle patch for scar-prevention healing of diabetic wounds
Science Advances, Jul 14, 2023; DOI: 10.1126/sciadv.adh1415

Puromycilation assay
Authors: David Papadopoli and Ivan Topisirovic
Date: May 11, 2026，Views：407
Original protocol used in research article：
Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics
eLife, May 5, 2026; DOI: 10.7554/eLife.106587

Application of flow
Authors: David Wu, Yun Fang and Gökhan M Mutlu
Date: May 11, 2026，Views：70
Original protocol used in research article：
HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium
eLife, May 30, 2017; DOI: 10.7554/eLife.25217
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.