Protocols shared by authors via RaP service
Journal
Purification of S. aureus ClpC and ClpP
Authors: Marta Carroni, Bernd Bukau and Axel Mogk
Date: Mar 17, 2024，Views：828
Original protocol used in research article：
Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control
eLife, Nov 22, 2017; DOI: 10.7554/eLife.30120
Ferrozine Iron Assay
Author: Kuanyu Li
Date: Mar 12, 2024，Views：852
Original protocol used in research article：
Hormone replacement therapy for postmenopausal atherosclerosis is offset by late age iron deposition
eLife, Aug 10, 2023; DOI: 10.7554/eLife.80494
PCA and DCCM
Authors: Gustavo R. Perez-Lemus, Juan J. de Pablo, Fabian Bylehn, Cintia Menendez and Walter Alvarado
Date: Mar 11, 2024，Views：812
Original protocol used in research article：
Toward wide-spectrum antivirals against coronaviruses: Molecular characterization of SARS-CoV-2 NSP13 helicase inhibitors
Science Advances, Jan 7, 2022; DOI: 10.1126/sciadv.abj4526
TI and strain calculations
Authors: Gustavo R. Perez-Lemus, Juan J. de Pablo, Fabian Bylehn, Cintia Menendez and Walter Alvarado
Date: Mar 11, 2024，Views：871
Original protocol used in research article：
Toward wide-spectrum antivirals against coronaviruses: Molecular characterization of SARS-CoV-2 NSP13 helicase inhibitors
Science Advances, Jan 7, 2022; DOI: 10.1126/sciadv.abj4526
Encapsulation Efficiency (EE) and Loading Efficiency (LE) Protocol
Author: Jiyun Shi
Date: Mar 11, 2024，Views：664
Original protocol used in research article：
A Dual pH-Responsive DOX-Encapsulated Liposome Combined with Glucose Administration Enhanced Therapeutic Efficacy of Chemotherapy for Cancer
International Journal of Nanomedicine, May 10, 2021; DOI: 10.2147/IJN.S303874
Synthesis of FCS
Authors: Xiaofeng Zhang, Ping Gu, Qian Chen and Zhuang Liu
Date: Mar 2, 2024，Views：694
Original protocol used in research article：
Eyedrop-based macromolecular ophthalmic drug delivery for ocular fundus disease treatment
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.abq3104
Liquid food consumption
Author: Patrick T McGrath
Date: Mar 1, 2024，Views：669
Original protocol used in research article：
Changes to social feeding behaviors are not sufficient for fitness gains of the Caenorhabditis elegans N2 reference strain
eLife, Oct 17, 2018; DOI: 10.7554/eLife.38675
Half-Life/Cycloheximide Chase Assay
Authors: Ritu Mishra and Akhil C. Banerjea
Date: Feb 29, 2024，Views：592
Original protocol used in research article：
SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia
Frontiers in Immunology, Apr 14, 2021; DOI: 10.3389/fimmu.2021.656700
Murine interferon-γ enzyme-linked immunosorbent spot assay
Authors: Uri Elia and Dan Peer
Date: Feb 29, 2024，Views：19
Original protocol used in research article：
A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium
Science Advances, Mar 8, 2023; DOI: 10.1126/sciadv.adg1036
Protein sample preparation from Drosophila embryos for Western blot analysis
Author: Ufuk Günesdogan
Date: Feb 16, 2024，Views：1350
Original protocol used in research article：
Histone supply regulates S phase timing and cell cycle progression
eLife, Sep 9, 2014; DOI: 10.7554/eLife.02443
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