Protocols shared by authors via RaP service
Journal
Organoid Seeding Protocol on Chips for MEA recordings
Authors: Michele Bertacchi and Michèle Studer
Date: Oct 26, 2025，Views：663
Original protocol used in research article：
FGF8-mediated gene regulation affects regional identity in human cerebral organoids
eLife, Nov 1, 2024; DOI: 10.7554/eLife.98096
Sample preparation for light sheet microscopy
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：306
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
In vivo light sheet imaging
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：46
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
Fluorescence-based ion transport assays using Proteoliposomes
Authors: Cristina Manatschal and Karthik Ramanadane
Date: Sep 20, 2025，Views：419
Original protocol used in research article：
Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family
eLife, Jan 10, 2022; DOI: 10.7554/eLife.74589
Mounting
Authors: John Darby Cole and Sebastian Jessberger
Date: Sep 18, 2025，Views：214
Original protocol used in research article：
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging
eLife, Jan 28, 2022; DOI: 10.7554/eLife.68000
Tracer injections
Authors: Natasha O'Brown, Sean G Megason and Chenghua Gu
Date: Sep 12, 2025，Views：230
Original protocol used in research article：
Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326
Principle component analysis (PCA)
Authors: Kingsley Wu, Ta I Hung and Chia-en A Chang
Date: Sep 11, 2025，Views：255
Original protocol used in research article：
PROTAC-induced protein structural dynamics in targeted protein degradation
eLife, Feb 27, 2025; DOI: 10.7554/eLife.101127
ChIP-seq analysis
Authors: Liang Xu, Ye Chen and Tuan Zea Tan
Date: Sep 9, 2025，Views：174
Original protocol used in research article：
Topography of transcriptionally active chromatin in glioblastoma
Science Advances, Apr 30, 2021; DOI: 10.1126/sciadv.abd4676
Liposome reconstitution
Authors: David N Nathan Tippett and Raimund Dutzler
Date: Aug 26, 2025，Views：294
Original protocol used in research article：
Structural and functional properties of the transporter SLC26A6 reveal mechanism of coupled anion exchange
eLife, Jun 23, 2023; DOI: 10.7554/eLife.87178
Identification of putative enhancers
Authors: Xiaotong Wu and Wei Xie
Date: Aug 22, 2025，Views：7
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.