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Preprint

Application of flow

DW David Wu
YF Yun Fang
GM Gökhan M Mutlu

Last updated date: May 11, 2026 Views: 2 Forks: 0

original An abbreviated version of this protocol was published in eLife in May, 2017
HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium
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All of the experimental details can be found in the publication below:

 

https://pubmed.ncbi.nlm.nih.gov/26034042/

Copyright: © 2026 The Author(s); This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Wu, D, Fang, Y and Mutlu, G(2026). Application of flow. Bio-protocol Preprint. bio-protocol.org/prep2946.
  2. Wu, D., Huang, R., Hamanaka, R. B., Krause, M., Oh, M., Kuo, C., Nigdelioglu, R., Meliton, A. Y., Witt, L., Dai, G., Civelek, M., Prabhakar, N. R., Fang, Y. and Mutlu, G. M.(2017). HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium. eLife. DOI: 10.7554/eLife.25217

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