Protocols shared by authors via RaP service
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MINFLUX data postprocessing
Authors: Qi Gao, Elmar Schiebel, Stefan Pfeffer and Charlotte Kaplan
Date: May 11, 2026，Views：312
Original protocol used in research article：
Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC
Nature Communications, Mar 12, 2025; DOI: 10.1038/s41467-025-57729-2

3xFLAG-eYFP plasmid sequences: Full reference plasmid sequence and local 5'UTR sequences
Authors: Camila Garcia and Candice E. Paulsen
Date: May 8, 2026，Views：55
Original protocol used in research article：
5′untranslated regions provide a versatile toolkit for tunable exogenous protein expression
Molecular Biology of the Cell, Mar 9, 2026; DOI: 10.1091/mbc.E25-12-0588

F/G-actin ratio
Authors: Lindsay K Festa and Olimpia Meucci
Date: May 7, 2026，Views：387
Original protocol used in research article：
CXCL12-induced rescue of cortical dendritic spines and cognitive flexibility
eLife, Jan 23, 2020; DOI: 10.7554/eLife.49717

Main Olfactory Epithelium/Bulb Extraction Protocol
Authors: Clara Wager Liff, Eliza Jaeger and Bianca Jones Marlin
Date: May 6, 2026，Views：305
Original protocol used in research article：
Fear conditioning biases olfactory sensory neuron frequencies across generations
eLife, Apr 14, 2026; DOI: 10.7554/eLife.92882

Puromycin incorporation assay (SUnSET assay)
Authors: Juan Zhang and Qiang Liu
Date: Apr 26, 2026，Views：84
Original protocol used in research article：
Kin17 promotes rDNA transcription, ribosomal biogenesis, and cortical lamination
EMBO Reports, Jul 17, 2025; DOI: 10.1038/s44319-025-00524-3

3.12. Non‐Human Primates and Intrathecal Procedure
Authors: Brenda Wan Shing Lam, Melissa Tan and Minh T N Le
Date: Apr 26, 2026，Views：266
Original protocol used in research article：
Extracellular Vesicles Administered via Intrathecal Injection Mediate Safe Delivery of Nucleic Acids to the Central Nervous System for Gene Therapy
Journal of Extracellular Vesicles, Jul 7, 2025; DOI: 10.1002/jev2.70116

Protein extraction
Author: Hayashi Yamamoto
Date: Apr 22, 2026，Views：304
Original protocol used in research article：
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
eLife, ; DOI: 10.7554/eLife.78923

4.4. DPPH Assay
Authors: Phraepakaporn Kunnaja, Seewaboon Sireeratawong, Sunee Chansakaow, Absorn Wittayapraparat and Pedcharada Yusuk
Date: Apr 21, 2026，Views：3359
Original protocol used in research article：
In Vitro Antioxidant Activity of Litsea martabanica Root Extract and Its Hepatoprotective Effect on Chlorpyrifos-Induced Toxicity in Rats
Molecules, Mar 28, 2021; DOI: 10.3390/molecules26071906

2.3. PBMC Isolation, Culture, and RNA Extraction
Authors: Lene Salimans, Ivan Bautmans and Rose Njemini
Date: Apr 21, 2026，Views：212
Original protocol used in research article：
Inflammation-Related Genes Are Differentially Expressed in Lipopolysaccharide-Stimulated Peripheral Blood Mononuclear Cells after 3 Months of Resistance Training in Older Women
Cells, Aug 25, 2024; DOI: 10.3390/cells13171416

Phospholipase A2 (PLA2) assay
Authors: R. R. Senji Laxme and Kartik Sunagar
Date: Apr 18, 2026，Views：7
Original protocol used in research article：
Biogeographical venom variation in the Indian spectacled cobra (Naja naja) underscores the pressing need for pan-India efficacious snakebite therapy
PLoS Neglected Tropical Diseases, Feb 18, 2021; DOI: 10.1371/journal.pntd.0009150
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