Protocols shared by authors via RaP service
Journal
In-gel fluorescence imaging and immunoblotting
Authors: Hayashi Yamamoto and Noboru Mizushima
Date: Apr 19, 2024，Views：254
Original protocol used in research article：
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
eLife, ; DOI: 10.7554/eLife.78923
Lead contact
Authors: Wen Fang and Liping Wang
Date: Apr 18, 2024，Views：65
Original protocol used in research article：
Neural dynamics of causal inference in the macaque frontoparietal circuit
eLife, Oct 24, 2022; DOI: 10.7554/eLife.76145
Cryo-Sectioning of viral tracer-labeled macaque brains
Authors: Mingchao Yan and Zheng Wang
Date: Apr 15, 2024，Views：281
Original protocol used in research article：
Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution and comparison with diffusion tractography
eLife, ; DOI: 10.7554/eLife.72534
Protocol for preparation of Quantum dot loaded dissolvable microneedle patches
Authors: Ana Jaklenec, Robert Langer and Kevin J. McHugh
Date: Apr 11, 2024，Views：414
Original protocol used in research article：
Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination
Science Translational Medicine, Dec 17, 2019; DOI: 10.1126/scitranslmed.aay7162
Stimulus sets in the study entitled 'Emerged human-like facial expression representation in a deep convolutional neural network'
Authors: Liqin Zhou, Ming Meng, Ke Zhou and Anmin Yang
Date: Apr 10, 2024，Views：17
Original protocol used in research article：
Emerged human-like facial expression representation in a deep convolutional neural network
Science Advances, Mar 23, 2022; DOI: 10.1126/sciadv.abj4383
Quantitative assessment of Stat92E-GFP expression and anti–p-Stat staining during oogenesis
Authors: Jhen-Wei Wu, Yu-Chiuan Chang and Anna C.-C. Jang
Date: Apr 1, 2024，Views：1
Original protocol used in research article：
Spatiotemporal gating of Stat nuclear influx by Drosophila Npas4 in collective cell migration
Science Advances, Jul 22, 2022; DOI: 10.1126/sciadv.abm2411
Isolation of Lamina Propria Cells from Mouse Small and Large Intestines
Authors: Qing Zhao and Charles O. Elson
Date: Mar 29, 2024，Views：540
Original protocol used in research article：
CD4+ T cell activation and concomitant mTOR metabolic inhibition can ablate microbiota-specific memory cells and prevent colitis
Science Immunology, Dec 11, 2020; DOI: 10.1126/sciimmunol.abc6373
Sulfate detection by ion-exchange chromatography
Authors: Maria Dalgaard Mikkelsen and Anne S. Meyer
Date: Mar 29, 2024，Views：8
Original protocol used in research article：
A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure
Scientific Reports, Sep 30, 2021; DOI: 10.1038/s41598-021-98588-3
T cell transwell migration
Authors: Scott B Thompson and Jordan Jacobelli
Date: Mar 27, 2024，Views：630
Original protocol used in research article：
Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046
Measurement of luciferase activity in tobacco
Author: Ken-ichiro Taoka
Date: Mar 26, 2024，Views：661
Original protocol used in research article：
Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc
Plant Biotechnology, Mar 25, 2021; DOI: 10.5511/plantbiotechnology.20.1209a
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