Protocols shared by authors via RaP service
Journal
Gene Set enrichment analysis
Authors: Hannah R Monday and Pablo Castillo
Date: Jan 14, 2026，Views：59
Original protocol used in research article：
CB1-receptor-mediated inhibitory LTD triggers presynaptic remodeling via protein synthesis and ubiquitination
eLife, Sep 9, 2020; DOI: 10.7554/eLife.54812
Culture conditions for new cell lines
Authors: Norbert Perrimon and Amanda Simcox
Date: Jan 9, 2026，Views：89
Original protocol used in research article：
Continuous muscle, glial, epithelial, neuronal, and hemocyte cell lines for Drosophila research
eLife, Jul 20, 2023; DOI: 10.7554/eLife.85814
Calculation of blood oxygen saturation
Authors: Wubin Bai, Wei Lu, Zhi-Dong Ge and John A. Rogers
Date: Jan 2, 2026，Views：115
Original protocol used in research article：
Wireless implantable catheter-type oximeter designed for cardiac oxygen saturation
Science Advances, Feb 9, 2021; DOI: 10.1126/sciadv.abe0579
Microinjection of Synthetic Peptides into Caenorhabditis elegans
Authors: Hayao Ohno, Takanori Ida and Yuichi Iino
Date: Dec 26, 2025，Views：169
Original protocol used in research article：
Luqin-like RYamide peptides regulate food-evoked responses in C. elegans
eLife, Aug 29, 2017; DOI: 10.7554/eLife.28877
Animals
Authors: Qian Chen, Zhuang Liu and Jingjing Shen
Date: Dec 24, 2025，Views：131
Original protocol used in research article：
Eyedrop-based macromolecular ophthalmic drug delivery for ocular fundus disease treatment
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.abq3104
Production of Nanobody 35
Authors: Fenghui Zhao, H Eric Xu, Dehua Yang, Lihua Zhao and Ming-Wei Wang
Date: Dec 21, 2025，Views：224
Original protocol used in research article：
Structural insights into hormone recognition by the human glucose-dependent insulinotropic polypeptide receptor
eLife, Jul 13, 2021; DOI: 10.7554/eLife.68719
Co-immunoprecipitation and western blotting
Author: Yukiko M Yamashita
Date: Dec 18, 2025，Views：150
Original protocol used in research article：
Alstrom syndrome gene is a stem-cell-specific regulator of centriole duplication in the Drosophila testis
eLife, Sep 23, 2020; DOI: 10.7554/eLife.59368
Flow cytometric analysis
Authors: Ambre Riba and Mathias W. Hornef
Date: Dec 17, 2025，Views：33
Original protocol used in research article：
Disturbed gut microbiota and bile homeostasis in Giardia-infected mice contributes to metabolic dysregulation and growth impairment
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.aay7019
Direct co-culture of parasites
Author: Natalia Hernan de Miguel
Date: Dec 11, 2025，Views：139
Original protocol used in research article：
Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication
eLife, May 2, 2023; DOI: 10.7554/eLife.86067
Nanobody screening by phage display
Authors: baolong xia and Norbert Perrimon
Date: Dec 3, 2025，Views：695
Original protocol used in research article：
Phage-displayed synthetic library and screening platform for nanobody discovery
eLife, Aug 1, 2025; DOI: 10.7554/eLife.105887
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