Bio-101 - Improve Research Reproducibility

Electrode holder implantation and transcranial direct current stimulation (tDCS)

Authors: Zheng Gan and Rohini Kuner
Date: Oct 15, 2023，Views：18

Original protocol used in research article：

Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391

Animal models

Authors: Sitong Zhang, Lingjun Tong, Huaxi Yi and Jiong-Wei Wang
Date: Oct 12, 2023，Views：501

Original protocol used in research article：

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041

gClustering and phylogenetic analyses

Authors: Mariana G López and Iñaki Comas
Date: Oct 9, 2023，Views：281

Original protocol used in research article：

Population-based sequencing of Mycobacterium tuberculosis reveals how current population dynamics are shaped by past epidemics
eLife, Jul 26, 2022; DOI: 10.7554/eLife.76605

Detection and Analysis of human ALDH2 by quantitative RT-PCR

Authors: Yao-Hui Gao, Guo-Qiang Chen and Li-Shun Wang
Date: Oct 7, 2023，Views：302

Original protocol used in research article：

VHL deficiency augments anthracycline sensitivity of clear cell renal cell carcinomas by down-regulating ALDH2
Nature Communications, Jun 15, 2017; DOI: 10.1038/ncomms15337

ANR, LR, and primary root phenotyping assays

Authors: Kun-Peng Jia and Salim Al-Babili
Date: Oct 6, 2023，Views：11

Original protocol used in research article：

Anchorene is a carotenoid-derived regulatory metabolite required for anchor root formation in Arabidopsis
Science Advances, Nov 26, 2019; DOI: 10.1126/sciadv.aaw6787

DNA processing and Duplex-Seq

Authors: Mariya T Sweetwyne and Scott R Kennedy
Date: Sep 30, 2023，Views：305

Original protocol used in research article：

The multi-tissue landscape of somatic mtDNA mutations indicates tissue-specific accumulation and removal in aging
eLife, Feb 17, 2023; DOI: 10.7554/eLife.83395

Pfs25 and PfMGET RNA quantification

Authors: Isaie J Reuling and Teun Bousema
Date: Sep 29, 2023，Views：215

Original protocol used in research article：

A randomized feasibility trial comparing four antimalarial drug regimens to induce Plasmodium falciparum gametocytemia in the controlled human malaria infection model
eLife, Feb 27, 2018; DOI: 10.7554/eLife.31549

CODEX antibody validation, titration, and staining

Author: Christian M. Schürch
Date: Sep 27, 2023，Views：9

Original protocol used in research article：

A tissue atlas of ulcerative colitis revealing evidence of sex-dependent differences in disease-driving inflammatory cell types and resistance to TNF inhibitor therapy
Science Advances, Jan 20, 2023; DOI: 10.1126/sciadv.add1166

CFSE-MLR and cell sorting protocol to define alloreactive T cell repertoires by high throughput TCR sequencing

Authors: Jianing Fu and Megan Sykes
Date: Sep 26, 2023，Views：63

Original protocol used in research article：

Lymphohematopoietic graft-versus-host responses promote mixed chimerism in patients receiving intestinal transplantation
The Journal of Clinical Investigation, Apr 15, 2021; DOI: 10.1172/jci141698

Viral copy number

Authors: Paula Rodriguez-Marquez, Juan R. Rodriguez-Madoz and Felipe Prosper
Date: Sep 26, 2023，Views：25

Original protocol used in research article：

CAR density influences antitumoral efficacy of BCMA CAR T cells and correlates with clinical outcome
Science Advances, Sep 30, 2022; DOI: 10.1126/sciadv.abo0514

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