Protocols shared by authors via RaP service
Journal
Sample preparation for light sheet microscopy
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：470
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
In vivo light sheet imaging
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：178
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
Fluorescence-based ion transport assays using Proteoliposomes
Authors: Cristina Manatschal and Karthik Ramanadane
Date: Sep 20, 2025，Views：827
Original protocol used in research article：
Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family
eLife, Jan 10, 2022; DOI: 10.7554/eLife.74589
Mounting
Authors: John Darby Cole and Sebastian Jessberger
Date: Sep 18, 2025，Views：380
Original protocol used in research article：
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging
eLife, Jan 28, 2022; DOI: 10.7554/eLife.68000
Tracer injections
Authors: Natasha O'Brown, Sean G Megason and Chenghua Gu
Date: Sep 12, 2025，Views：406
Original protocol used in research article：
Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326
Principle component analysis (PCA)
Authors: Kingsley Wu, Ta I Hung and Chia-en A Chang
Date: Sep 11, 2025，Views：418
Original protocol used in research article：
PROTAC-induced protein structural dynamics in targeted protein degradation
eLife, Feb 27, 2025; DOI: 10.7554/eLife.101127
ChIP-seq analysis
Authors: Liang Xu, Ye Chen and Tuan Zea Tan
Date: Sep 9, 2025，Views：330
Original protocol used in research article：
Topography of transcriptionally active chromatin in glioblastoma
Science Advances, Apr 30, 2021; DOI: 10.1126/sciadv.abd4676
Liposome reconstitution
Authors: David N Nathan Tippett and Raimund Dutzler
Date: Aug 26, 2025，Views：691
Original protocol used in research article：
Structural and functional properties of the transporter SLC26A6 reveal mechanism of coupled anion exchange
eLife, Jun 23, 2023; DOI: 10.7554/eLife.87178
Fluorescent BODIPY-FL vancomycin staining
Authors: Moagi Tube Shaku and Bavesh D Kana
Date: Aug 8, 2025，Views：518
Original protocol used in research article：
A modified BCG with depletion of enzymes associated with peptidoglycan amidation induces enhanced protection against tuberculosis in mice
eLife, Apr 19, 2024; DOI: 10.7554/eLife.89157
Detection of CMV pp65-specific T cells by intracellular staining with anti-INF-γ-PE
Author: David Klatzmann
Date: Aug 1, 2025，Views：357
Original protocol used in research article：
Human thymopoiesis produces polyspecific CD8+ α/β T cells responding to multiple viral antigens
eLife, Mar 30, 2023; DOI: 10.7554/eLife.81274
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