Protocols shared by authors via RaP service
Journal
Drosophila ovary extraction, fixation and immunostaining protocol
Authors: Wen Lu and Vladimir I. Gelfand
Date: Oct 31, 2024，Views：157
Original protocol used in research article：
A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary
eLife, Feb 16, 2022; DOI: 10.7554/eLife.75538
4.4. Eriochrome Cyanine Staining
Authors: Rocío Benítez-Fernández, Ana Martínez and Fernando de Castro
Date: Oct 28, 2024，Views：131
Original protocol used in research article：
Dynamics of Central Remyelination and Treatment Evolution in a Model of Multiple Sclerosis with Optic Coherence Tomography
International Journal of Molecular Sciences, Feb 28, 2021; DOI: 10.3390/ijms22052440
Proteoliposome-based in vitro transport assays
Authors: Shamayeeta Ray and Rachelle Gaudet
Date: Oct 27, 2024，Views：285
Original protocol used in research article：
High-resolution structures with bound Mn2+ and Cd2+ map the metal import pathway in an Nramp transporter
eLife, Apr 11, 2023; DOI: 10.7554/eLife.84006
Co-immunoprecipitation from S2 cells
Author: Richard G Fehon
Date: Oct 26, 2024，Views：109
Original protocol used in research article：
Negative feedback couples Hippo pathway activation with Kibra degradation independent of Yorkie-mediated transcription
eLife, Feb 8, 2021; DOI: 10.7554/eLife.62326
Quantification of cytosolic DNA
Authors: Haitao Wen, JIANWEN CHEN and Hong Dong
Date: Oct 24, 2024，Views：134
Original protocol used in research article：
Inhibition of O-GlcNAc transferase activates type I interferon-dependent antitumor immunity by bridging cGAS-STING pathway
eLife, Oct 4, 2024; DOI: 10.7554/eLife.94849
Cell culture
Author: Yoko Nakai-Futatsugi
Date: Oct 12, 2024，Views：68
Original protocol used in research article：
Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile
eLife, May 9, 2024; DOI: 10.7554/eLife.92510
Expansion of hPS Cell Derived Pancreatic Progenitors
Author: Anthony Gavalas
Date: Oct 9, 2024，Views：159
Original protocol used in research article：
Regulation of multiple signaling pathways promotes the consistent expansion of human pancreatic progenitors in defined conditions
eLife, Jan 5, 2024; DOI: 10.7554/eLife.89962
Protocol for FilmTracer™ SYPRO® Ruby biofilm matrix stain
Author: Ronit Vogt Sionov
Date: Sep 23, 2024，Views：207
Original protocol used in research article：
Tooth mousse containing casein phosphopeptide-amorphous calcium phosphate prevents biofilm formation of Streptococcus mutans
BMC Oral Health, Mar 19, 2021; DOI: 10.1186/s12903-021-01502-6
Primary keratinocyte cell culture
Author: Cheryl L Stucky
Date: Sep 3, 2024，Views：179
Original protocol used in research article：
Keratinocytes mediate innocuous and noxious touch via ATP-P2X4 signaling
eLife, Jan 16, 2018; DOI: 10.7554/eLife.31684
Isolation of PDGFRβ+ REPs
Authors: Guangfeng Geng, Quan Chen, Yushan Zhu and Lihong Shi
Date: Aug 15, 2024，Views：118
Original protocol used in research article：
Receptor-mediated mitophagy regulates EPO production and protects against renal anemia
eLife, May 4, 2021; DOI: 10.7554/eLife.64480
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.