Bio-101 - Improve Research Reproducibility

mtDNA extraction from Fr8 EVs and qPCR

Author: Efrat Levy
Date: Apr 28, 2021，Views：893

Original protocol used in research article：

Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in Down syndrome
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abe5085

In-situ proximity ligation

Authors: Konrad Basler, Andreas E Moor, Claudio Cantù and Amaia Jauregi-Miguel
Date: Apr 28, 2021，Views：819

Original protocol used in research article：

TBX3 acts as tissue-specific component of the Wnt/β-catenin transcriptional complex
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58123

Microfabrication of microfluidic molds for layer-specific microchannel networks

Author: Tyler R Ray
Date: Apr 27, 2021，Views：852

Original protocol used in research article：

Soft, skin-interfaced sweat stickers for cystic fibrosis diagnosis and management
Science Translational Medicine, Mar 31, 2021; DOI: 10.1126/scitranslmed.abd8109

Neural induction

Authors: G. Zhao and J. Fu
Date: Apr 26, 2021，Views：891

Original protocol used in research article：

Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI: 10.1126/sciadv.aax5933

Fabrication of gel beds

Authors: G. Zhao and J. Fu
Date: Apr 26, 2021，Views：778

Original protocol used in research article：

Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI: 10.1126/sciadv.aax5933

Confocal microscopy and analysis

Authors: Maria D Purice, Sean D Speese and Mary A Logan
Date: Apr 26, 2021，Views：1159

Original protocol used in research article：

A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade
eLife, Aug 21, 2017; DOI: 10.7554/eLife.23611

CNV detection

Authors: Jean-Laurent Casanova and Qian Zhang
Date: Apr 26, 2021，Views：898

Original protocol used in research article：

Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570

Influenza virus protein

Authors: Jean-Laurent Casanova and Qian Zhang
Date: Apr 26, 2021，Views：1014

Original protocol used in research article：

Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570

Patients

Authors: Qian Zhang and Jean-Laurent Casanova
Date: Apr 26, 2021，Views：1005

Original protocol used in research article：

Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570

Mouse brain stereotaxic injections

Authors: Huda Y. Zoghbi, Yingyao Shao and Yehezkel Sztainberg
Date: Apr 26, 2021，Views：2243

Original protocol used in research article：

Antisense oligonucleotide therapy in a humanized mouse model of MECP2 duplication syndrome
Science Translational Medicine, Mar 2, 2021; DOI: 10.1126/scitranslmed.aaz7785

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