Protocols shared by authors via RaP service
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Quantitative assessment of Stat92E-GFP expression and anti–p-Stat staining during oogenesis
Authors: Jhen-Wei Wu, Yu-Chiuan Chang and Anna C.-C. Jang
Date: Apr 1, 2024，Views：6
Original protocol used in research article：
Spatiotemporal gating of Stat nuclear influx by Drosophila Npas4 in collective cell migration
Science Advances, Jul 22, 2022; DOI: 10.1126/sciadv.abm2411
Isolation of Lamina Propria Cells from Mouse Small and Large Intestines
Authors: Qing Zhao and Charles O. Elson
Date: Mar 29, 2024，Views：634
Original protocol used in research article：
CD4+ T cell activation and concomitant mTOR metabolic inhibition can ablate microbiota-specific memory cells and prevent colitis
Science Immunology, Dec 11, 2020; DOI: 10.1126/sciimmunol.abc6373
Sulfate detection by ion-exchange chromatography
Authors: Maria Dalgaard Mikkelsen and Anne S. Meyer
Date: Mar 29, 2024，Views：14
Original protocol used in research article：
A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure
Scientific Reports, Sep 30, 2021; DOI: 10.1038/s41598-021-98588-3
T cell transwell migration
Authors: Scott B Thompson and Jordan Jacobelli
Date: Mar 27, 2024，Views：759
Original protocol used in research article：
Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046
Measurement of luciferase activity in tobacco
Author: Ken-ichiro Taoka
Date: Mar 26, 2024，Views：774
Original protocol used in research article：
Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc
Plant Biotechnology, Mar 25, 2021; DOI: 10.5511/plantbiotechnology.20.1209a
Analyses of single-molecule TIRF data
Authors: Deepti Karandur, Evan R Williams and John Kuriyan
Date: Mar 26, 2024，Views：30
Original protocol used in research article：
Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
eLife, Sep 9, 2020; DOI: 10.7554/eLife.57784
Immunostaining protocol of human 3D bioengineered skeletal muscle whole mounts and cross section for confocal microscopy
Authors: Arne D Hofemeier and Timo Betz
Date: Mar 26, 2024，Views：749
Original protocol used in research article：
Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
eLife, Jan 18, 2021; DOI: 10.7554/eLife.60145
Adult rat DRG neuron culture
Authors: Dong Wang, Susu Mao and Bin Yu
Date: Mar 25, 2024，Views：779
Original protocol used in research article：
Promoting axon regeneration by inhibiting RNA N6-methyladenosine demethylase ALKBH5
eLife, Aug 3, 2023; DOI: 10.7554/eLife.85309
Recombinant expression and purification of human EP300-KIX87 domain from Escherichia coli
Authors: John H. Bushweller, Kwon-Sik Park and Ashish Kabra
Date: Mar 21, 2024，Views：842
Original protocol used in research article：
KIX domain determines a selective tumor-promoting role for EP300 and its vulnerability in small cell lung cancer
Science Advances, Feb 16, 2022; DOI: 10.1126/sciadv.abl4618
Construct design
Authors: Arpita Bharadwaj and Isabelle Baconguis
Date: Mar 20, 2024，Views：831
Original protocol used in research article：
Structure of the human epithelial sodium channel by cryo-electron microscopy
eLife, Sep 25, 2018; DOI: 10.7554/eLife.39340
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