Bio-101 - Improve Research Reproducibility

Reduced Morningness-Eveningness Questionnaire (rMEQ)

Author: Michael Rönnlund
Date: Nov 15, 2023，Views：47

Original protocol used in research article：

A Time to Sleep Well and Be Contented: Time Perspective, Sleep Quality, and Life Satisfaction
Frontiers in Psychology, Apr 16, 2021; DOI: 10.3389/fpsyg.2021.627836

Sequence retrieval and variant calling

Authors: Saba Naz and Vinay Kumar Nandicoori
Date: Nov 13, 2023，Views：2571

Original protocol used in research article：

GWAS and functional studies suggest a role for altered DNA repair in the evolution of drug resistance in Mycobacterium tuberculosis
eLife, Jan 25, 2023; DOI: 10.7554/eLife.75860

Cycloheximide study

Author: Irina Giurgea
Date: Nov 12, 2023，Views：9

Original protocol used in research article：

A critical region of A20 unveiled by missense TNFAIP3 variations that lead to autoinflammation
eLife, Jun 21, 2023; DOI: 10.7554/eLife.81280

Determination of total triterpenoid content

Authors: Marija R. Mandić, Bojan Dj. Božić and Biljana Dj. Božić Nedeljković
Date: Nov 9, 2023，Views：40

Original protocol used in research article：

Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia
PLoS ONE, Feb 11, 2021; DOI: 10.1371/journal.pone.0246810

Pseudovirus (PSV) Assay

Authors: Thomas F. Rogers, Devin Sok, Joseph G. Jardine and Dennis R. Burton
Date: Nov 6, 2023，Views：2261

Original protocol used in research article：

Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
Science, Aug 21, 2020; DOI: 10.1126/science.abc7520

Determination of site‐specific m6A modification levels using SELECT

Author: Tian‐Liang Xia
Date: Nov 4, 2023，Views：1822

Original protocol used in research article：

N(6)‐methyladenosine‐binding protein YTHDF1 suppresses EBV replication and promotes EBV RNA decay
EMBO Reports, Feb 19, 2021; DOI: 10.15252/embr.202050128

Pharyngeal Pumping Assay (Drosophila melanogaster)

Authors: John R Carlson and Ryan M Joseph
Date: Nov 3, 2023，Views：1577

Original protocol used in research article：

A receptor and neuron that activate a circuit limiting sucrose consumption
eLife, Mar 23, 2017; DOI: 10.7554/eLife.24992

2.4. Amoeba–Bacteria Co-Culture

Authors: Mohamed Shaheen and Nicholas J. Ashbolt
Date: Nov 2, 2023，Views：1537

Original protocol used in research article：

Differential Bacterial Predation by Free-Living Amoebae May Result in Blooms of Legionella in Drinking Water Systems
Microorganisms, Jan 15, 2021; DOI: 10.3390/microorganisms9010174

Isolation and characterization of mEVs and EV-depleted supernatant

Authors: Lingjun Tong, Jiong-Wei Wang and Sitong Zhang
Date: Oct 26, 2023，Views：1086

Original protocol used in research article：

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041

Protein extraction and Macf1 identification by western blot

Authors: Alireza Ghasemizadeh and Vincent Gache
Date: Oct 26, 2023，Views：1072

Original protocol used in research article：

MACF1 controls skeletal muscle function through the microtubule-dependent localization of extra-synaptic myonuclei and mitochondria biogenesis
eLife, Aug 27, 2021; DOI: 10.7554/eLife.70490

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