Bio-101 - Improve Research Reproducibility

Protein sample preparation from Drosophila embryos for Western blot analysis

Author: Ufuk Günesdogan
Date: Feb 16, 2024，Views：1458

Original protocol used in research article：

Histone supply regulates S phase timing and cell cycle progression
eLife, Sep 9, 2014; DOI: 10.7554/eLife.02443

Lentivirus production and transduction

Authors: Wahyu Handoko Wibowo Darsono, Seung-Jae Lee and Sang-Hun Lee
Date: Feb 16, 2024，Views：1161

Original protocol used in research article：

Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson’s disease-linked DNAJC6 mutations
Science Advances, Feb 16, 2021; DOI: 10.1126/sciadv.abb1540

Internalization assay

Authors: Elena Gammella, Alexia Conte, Gaetano Cairo and Stefania Recalcati
Date: Feb 16, 2024，Views：996

Original protocol used in research article：

Unconventional endocytosis and trafficking of transferrin receptor induced by iron
Molecular Biology of the Cell, Jan 15, 2021; DOI: 10.1091/mbc.E20-02-0129

Dual luciferase assay

Authors: Maija Pollari and Kristiina Mäkinen
Date: Feb 9, 2024，Views：924

Original protocol used in research article：

The potyviral silencing suppressor HCPro recruits and employs host ARGONAUTE1 in pro-viral functions
PLoS Pathogens, Oct 8, 2020; DOI: 10.1371/journal.ppat.1008965

Myelin isolation and staining

Authors: Rasmus Berglund and Maja Jagodic
Date: Feb 8, 2024，Views：1003

Original protocol used in research article：

Microglial autophagy–associated phagocytosis is essential for recovery from neuroinflammation
Science Immunology, Oct 15, 2020; DOI: 10.1126/sciimmunol.abb5077

HSPC and MPP2 isolation, barcoding, EPO treatment, and transplantation

Authors: Leïla Perié, Jason Cosgrove and Almut Eisele
Date: Feb 7, 2024，Views：931

Original protocol used in research article：

Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells
eLife, Feb 15, 2022; DOI: 10.7554/eLife.66922

BSH Activity Assays.

Authors: Rodolphe Barrangou and Casey M. Theriot
Date: Feb 1, 2024，Views：1018

Original protocol used in research article：

Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization
Proceedings of the National Academy of Sciences of the United States of America, Feb 1, 2021; DOI: 10.1073/pnas.2017709118

Transgenic tomato plants

Authors: A. Stintzi and A. Schaller
Date: Jan 30, 2024，Views：1061

Original protocol used in research article：

Peptide signaling for drought-induced tomato flower drop
Science, Mar 26, 2020; DOI: 10.1126/science.aaz5641

Isolation of human DRG nuclei

Authors: Minh Q Nguyen, Nicholas JP Ryba and Steve Davidson
Date: Jan 30, 2024，Views：1037

Original protocol used in research article：

Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752

PCL nerve conduit fabrication

Authors: Neil B. Fadia and Kacey G. Marra
Date: Jan 26, 2024，Views：1115

Original protocol used in research article：

Long-gap peripheral nerve repair through sustained release of a neurotrophic factor in nonhuman primates
Science Translational Medicine, Jan 21, 2020; DOI: 10.1126/scitranslmed.aav7753

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