Protocols shared by authors via RaP service
Journal
Reaching steady state in simulations
Authors: Jeff Gore and Jiliang Hu
Date: Dec 22, 2023，Views：14
Original protocol used in research article：
Emergent phases of ecological diversity and dynamics mapped in microcosms
Science, Oct 6, 2022; DOI: 10.1126/science.abm7841
Bacterial strains
Authors: Zilu Qu, Lei Yin, Xiao-Lian Zhang and Zilu Qu
Date: Dec 21, 2023，Views：9
Original protocol used in research article：
Mycobacterial EST12 activates a RACK1–NLRP3–gasdermin D pyroptosis–IL-1β immune pathway
Science Advances, Oct 22, 2020; DOI: 10.1126/sciadv.aba4733
RNA isolation and qPCR
Authors: Raymundo D Hernandez and Michelle L Olsen
Date: Dec 20, 2023，Views：2246
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
Single-Cell TCR Sequencing
Authors: Bernardo S. Reis and Daniel Mucida
Date: Dec 19, 2023，Views：2215
Original protocol used in research article：
TCR-Vγδ usage distinguishes protumor from antitumor intestinal γδ T cell subsets
Science, Jul 14, 2022; DOI: 10.1126/science.abj8695
IL-2/JES6-1 and IL-2/S4B6 complexes
Author: Marek Kovar
Date: Dec 19, 2023，Views：2247
Original protocol used in research article：
IL-2/JES6-1 mAb complexes dramatically increase sensitivity to LPS through IFN-γ production by CD25+Foxp3- T cells
eLife, Dec 21, 2021; DOI: 10.7554/eLife.62432
Multiparametric flow cytometry and CATALYST analysis
Authors: María M. Coronel, Haval Shirwan and Andrés J. García
Date: Dec 14, 2023，Views：2117
Original protocol used in research article：
Immunotherapy via PD-L1–presenting biomaterials leads to long-term islet graft survival
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.aba5573
PAS, AB, and AB-PAS staining
Authors: Andong Zhao and Xiaobing Fu
Date: Dec 13, 2023，Views：28
Original protocol used in research article：
Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy
Science Advances, Apr 14, 2021; DOI: 10.1126/sciadv.abb2213
Determination of membrane charge
Author: Andrew M Edwards
Date: Dec 12, 2023，Views：24
Original protocol used in research article：
Colistin kills bacteria by targeting lipopolysaccharide in the cytoplasmic membrane
eLife, Apr 6, 2021; DOI: 10.7554/eLife.65836
Immunofluorescent labelling of adult mouse neuromuscular junctions
Authors: Roberta Sartori, Adam Hagg, Paul Gregorevic and Marco Sandri
Date: Dec 7, 2023，Views：2249
Original protocol used in research article：
Perturbed BMP signaling and denervation promote muscle wasting in cancer cachexia
Science Translational Medicine, Aug 4, 2021; DOI: 10.1126/scitranslmed.aay9592
Mouse Aortic Ring Assay
Authors: Carmen Ruiz de Almodovar and Xiaohong Wang
Date: Dec 5, 2023，Views：2513
Original protocol used in research article：
Protein Phosphatase 2A Mediates YAP Activation in Endothelial Cells Upon VEGF Stimulation and Matrix Stiffness
Frontiers in Cell and Developmental Biology, May 13, 2021; DOI: 10.3389/fcell.2021.675562
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