Protocols shared by authors via RaP service
Journal
Mouse B cell culture
Authors: Roberta Buono and David A. Fruman
Date: Jul 1, 2024，Views：132
Original protocol used in research article：
Reduced eIF4E function impairs B-cell leukemia without altering normal B-lymphocyte function
iScience, Jun 17, 2021; DOI: 10.1016/j.isci.2021.102748
How to make anti-IgA beads for serum IgA purification
Authors: Yoshihito Nihei and Daisuke Kitamura
Date: Jun 25, 2024，Views：105
Original protocol used in research article：
Identification of IgA autoantibodies targeting mesangial cells redefines the pathogenesis of IgA nephropathy
Science Advances, Mar 22, 2023; DOI: 10.1126/sciadv.add6734
RiboPuromycylation
Authors: Sangeetha Devi Kumar and Sandeep M Eswarappa
Date: Jun 22, 2024，Views：56
Original protocol used in research article：
Evidence for low-level translation in human erythrocytes
Molecular Biology of the Cell, Sep 22, 2022; DOI: 10.1091/mbc.E21-09-0437
RNA pull-down assay
Authors: Chuannan Fan and Peter ten Dijke
Date: Jun 13, 2024，Views：20
Original protocol used in research article：
The lncRNA LETS1 promotes TGF-β–induced EMT and cancer cell migration by transcriptionally activating a TβR1-stabilizing mechanism
SCIENCE SIGNALING, Jun 20, 2023; DOI: 10.1126/scisignal.adf1947
Intracytoplasmic sperm injection（ICSI）
Authors: Yongliang Shang, Fei Gao, Xuejiang Guo, Qing-Yuan Sun, Yunxia Cao and Wei Li
Date: Jun 7, 2024，Views：404
Original protocol used in research article：
Essential role for SUN5 in anchoring sperm head to the tail
eLife, Sep 25, 2017; DOI: 10.7554/eLife.28199
Biochemical measurement of Rab5 activity
Author: Muralidharan Mani
Date: Jun 4, 2024，Views：326
Original protocol used in research article：
Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling
Molecular Biology of the Cell, Jan 15, 2016; DOI: 10.1091/mbc.E15-08-0558
RNA isolation, cDNA synthesis, and quantitative RT-PCR
Authors: Keshav Thapa and Petteri Rinne
Date: Jun 4, 2024，Views：852
Original protocol used in research article：
Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver
eLife, Jul 25, 2023; DOI: 10.7554/eLife.84782
Mouse intestinal epithelial cell strip
Authors: Timothy J Nice and David A Constant
Date: May 24, 2024，Views：595
Original protocol used in research article：
Homeostatic interferon-lambda response to bacterial microbiota stimulates preemptive antiviral defense within discrete pockets of intestinal epithelium
eLife, Feb 9, 2022; DOI: 10.7554/eLife.74072
Ex vivo muscle function testing
Authors: Jeffrey Molendijk, Ronnie Blazev and Benjamin L Parker
Date: May 14, 2024，Views：517
Original protocol used in research article：
Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function
eLife, Dec 6, 2022; DOI: 10.7554/eLife.82951
Histology and immunofluorescence staining and analysis
Author: Derek C Sung
Date: May 13, 2024，Views：587
Original protocol used in research article：
VE-cadherin enables trophoblast endovascular invasion and spiral artery remodeling during placental development
eLife, Apr 29, 2022; DOI: 10.7554/eLife.77241
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