Protocols shared by authors via RaP service
Journal
T cell transwell migration
Authors: Scott B Thompson and Jordan Jacobelli
Date: Mar 27, 2024，Views：1067
Original protocol used in research article：
Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046
Measurement of luciferase activity in tobacco
Author: Ken-ichiro Taoka
Date: Mar 26, 2024，Views：843
Original protocol used in research article：
Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc
Plant Biotechnology, Mar 25, 2021; DOI: 10.5511/plantbiotechnology.20.1209a
Analyses of single-molecule TIRF data
Authors: Deepti Karandur, Evan R Williams and John Kuriyan
Date: Mar 26, 2024，Views：54
Original protocol used in research article：
Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
eLife, Sep 9, 2020; DOI: 10.7554/eLife.57784
Immunostaining protocol of human 3D bioengineered skeletal muscle whole mounts and cross section for confocal microscopy
Authors: Arne D Hofemeier and Timo Betz
Date: Mar 26, 2024，Views：817
Original protocol used in research article：
Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
eLife, Jan 18, 2021; DOI: 10.7554/eLife.60145
Adult rat DRG neuron culture
Authors: Dong Wang, Susu Mao and Bin Yu
Date: Mar 25, 2024，Views：816
Original protocol used in research article：
Promoting axon regeneration by inhibiting RNA N6-methyladenosine demethylase ALKBH5
eLife, Aug 3, 2023; DOI: 10.7554/eLife.85309
Recombinant expression and purification of human EP300-KIX87 domain from Escherichia coli
Authors: John H. Bushweller, Kwon-Sik Park and Ashish Kabra
Date: Mar 21, 2024，Views：862
Original protocol used in research article：
KIX domain determines a selective tumor-promoting role for EP300 and its vulnerability in small cell lung cancer
Science Advances, Feb 16, 2022; DOI: 10.1126/sciadv.abl4618
Construct design
Authors: Arpita Bharadwaj and Isabelle Baconguis
Date: Mar 20, 2024，Views：869
Original protocol used in research article：
Structure of the human epithelial sodium channel by cryo-electron microscopy
eLife, Sep 25, 2018; DOI: 10.7554/eLife.39340
Reporter design and virus production
Authors: Natalya N Pavlova and Craig B Thompson
Date: Mar 20, 2024，Views：857
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
Protein constructs, expression, and purification
Authors: Alexei Gorelik and Bhushan Nagar
Date: Mar 19, 2024，Views：870
Original protocol used in research article：
Structure of the immunoregulatory sialidase NEU1
Science Advances, May 19, 2023; DOI: 10.1126/sciadv.adf8169
UMAP and ICI analysis
Authors: Bruno Guillotin and Kenneth D. Birnbaum
Date: Mar 19, 2024，Views：42
Original protocol used in research article：
Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327
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