Bio-101 - Improve Research Reproducibility

Community network analysis

Authors: Francesco Colizzi and Modesto Orozco
Date: Mar 18, 2024，Views：25

Original protocol used in research article：

Probing allosteric regulations with coevolution-driven molecular simulations
Science Advances, Sep 8, 2021; DOI: 10.1126/sciadv.abj0786

Small RNA preparation and qPCR

Authors: Luxi Chen and Anming Meng
Date: Mar 18, 2024，Views：811

Original protocol used in research article：

5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
Science Advances, Nov 19, 2021; DOI: 10.1126/sciadv.abh0494

Purification of S. aureus ClpC and ClpP

Authors: Marta Carroni, Bernd Bukau and Axel Mogk
Date: Mar 17, 2024，Views：868

Original protocol used in research article：

Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control
eLife, Nov 22, 2017; DOI: 10.7554/eLife.30120

Ferrozine Iron Assay

Author: Kuanyu Li
Date: Mar 12, 2024，Views：939

Original protocol used in research article：

Hormone replacement therapy for postmenopausal atherosclerosis is offset by late age iron deposition
eLife, Aug 10, 2023; DOI: 10.7554/eLife.80494

PCA and DCCM

Authors: Gustavo R. Perez-Lemus, Juan J. de Pablo, Fabian Bylehn, Cintia Menendez and Walter Alvarado
Date: Mar 11, 2024，Views：854

Original protocol used in research article：

Toward wide-spectrum antivirals against coronaviruses: Molecular characterization of SARS-CoV-2 NSP13 helicase inhibitors
Science Advances, Jan 7, 2022; DOI: 10.1126/sciadv.abj4526

TI and strain calculations

Authors: Gustavo R. Perez-Lemus, Juan J. de Pablo, Fabian Bylehn, Cintia Menendez and Walter Alvarado
Date: Mar 11, 2024，Views：885

Original protocol used in research article：

Toward wide-spectrum antivirals against coronaviruses: Molecular characterization of SARS-CoV-2 NSP13 helicase inhibitors
Science Advances, Jan 7, 2022; DOI: 10.1126/sciadv.abj4526

Encapsulation Efficiency (EE) and Loading Efficiency (LE) Protocol

Author: Jiyun Shi
Date: Mar 11, 2024，Views：721

Original protocol used in research article：

A Dual pH-Responsive DOX-Encapsulated Liposome Combined with Glucose Administration Enhanced Therapeutic Efficacy of Chemotherapy for Cancer
International Journal of Nanomedicine, May 10, 2021; DOI: 10.2147/IJN.S303874

Synthesis of FCS

Authors: Xiaofeng Zhang, Ping Gu, Qian Chen and Zhuang Liu
Date: Mar 2, 2024，Views：727

Original protocol used in research article：

Eyedrop-based macromolecular ophthalmic drug delivery for ocular fundus disease treatment
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.abq3104

Liquid food consumption

Author: Patrick T McGrath
Date: Mar 1, 2024，Views：696

Original protocol used in research article：

Changes to social feeding behaviors are not sufficient for fitness gains of the Caenorhabditis elegans N2 reference strain
eLife, Oct 17, 2018; DOI: 10.7554/eLife.38675

Half-Life/Cycloheximide Chase Assay

Authors: Ritu Mishra and Akhil C. Banerjea
Date: Feb 29, 2024，Views：627

Original protocol used in research article：

SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia
Frontiers in Immunology, Apr 14, 2021; DOI: 10.3389/fimmu.2021.656700

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