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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1361)
Biophysics (158)
Cancer Biology (423)
Cell Biology (2085)
Developmental Biology (314)
Immunology (592)
Microbiology (1142)
Molecular Biology (1113)
Neuroscience (686)
Plant Science (1044)
Stem Cell (246)
Systems Biology (330)
Bio-protocol journal
Special issues
Lab protocol hub
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Turkish Journal of Pharmaceutical Sciences
Virulence
Transgenic tomato plants
Authors:
A. Stintzi
and
A. Schaller
Date: Jan 30, 2024,Views:1042
Original protocol used in research article:
Peptide signaling for drought-induced tomato flower drop
Science, Mar 26, 2020; DOI:
10.1126/science.aaz5641
Isolation of human DRG nuclei
Authors:
Minh Q Nguyen
,
Nicholas JP Ryba
and
Steve Davidson
Date: Jan 30, 2024,Views:1000
Original protocol used in research article:
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI:
10.7554/eLife.71752
PCL nerve conduit fabrication
Authors:
Neil B. Fadia
and
Kacey G. Marra
Date: Jan 26, 2024,Views:1099
Original protocol used in research article:
Long-gap peripheral nerve repair through sustained release of a neurotrophic factor in nonhuman primates
Science Translational Medicine, Jan 21, 2020; DOI:
10.1126/scitranslmed.aav7753
VEGF-A–targeting functional manipulations
Authors:
Ralf Paus
and
Amos Gilhar
Date: Jan 20, 2024,Views:1178
Original protocol used in research article:
Human organ rejuvenation by VEGF-A: Lessons from the skin
Science Advances, Jun 24, 2022; DOI:
10.1126/sciadv.abm6756
8× expansion protocol for the Drosophila CNS
Authors:
Joshua L Lillvis
,
Amy Hu
,
Paul W Tillberg
and
Barry J Dickson
Date: Jan 18, 2024,Views:1231
Original protocol used in research article:
Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy
eLife, Oct 26, 2022; DOI:
10.7554/eLife.81248
Immunofluorescence Protocol for HNF4α (P1 and P2 isoforms)
Authors:
Karthikeyani Chellappa
,
Poonamjot Deol
and
Frances M Sladek
Date: Jan 12, 2024,Views:1386
Original protocol used in research article:
Opposing roles of nuclear receptor HNF4α isoforms in colitis and colitis-associated colon cancer
eLife, May 11, 2016; DOI:
10.7554/eLife.10903
Estimation of the free energy of the Arg381-Asp410 salt bridge
Authors:
Yingjie Wang
,
Gianluigi Veglia
,
Dongping Zhong
and
Jiali Gao
Date: Jan 11, 2024,Views:12
Original protocol used in research article:
Activation mechanism of Drosophila cryptochrome through an allosteric switch
Science Advances, Jun 17, 2021; DOI:
10.1126/sciadv.abg3815
In situ hybridizations
Author:
Shelby A Blythe
Date: Jan 9, 2024,Views:1537
Original protocol used in research article:
Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the
Drosophila
segmentation network
eLife, Apr 29, 2020; DOI:
10.7554/eLife.53916
Glycerol gradient sedimentation and trichloroacetic acid precipitation of proteins
Authors:
Raquel Crespo
and
Tokameh Mahmoudi
Date: Jan 9, 2024,Views:1660
Original protocol used in research article:
Gliotoxin identified from a screen of fungal metabolites disrupts 7SK snRNP releases P-TEFb and reverses HIV-1 latency
Science Advances, Aug 11, 2020; DOI:
10.1126/sciadv.aba6617
4.9. Measurement of F-Actin/G-Actin Ratio
Authors:
Wei-Lun Liu
,
Meng-Hsuan Cheng
,
Inn-Wen Chong
and
Po-Len Liu
Date: Jan 4, 2024,Views:10
Original protocol used in research article:
High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1
International Journal of Molecular Sciences, Mar 31, 2021; DOI:
10.3390/ijms22073628
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