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Measurement of the fluorescence intensity of TCSn:GFP in the Arabidopsis root epidermis

NT Naoki Takahashi
MU Masaaki Umeda

Last updated date: Feb 2, 2025 DOI: 10.21769/p2787 Views: 24 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Jun, 2021
Alterations in hormonal signals spatially coordinate distinct responses to DNA double-strand breaks in Arabidopsis roots
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  1. Open the image using the Fiji image analysis software (http://fiji.sc).
  2. Launch the ROI Manager and enclose four epidermal cells above and below the boundary between the meristematic zone (MZ) and the transition zone (TZ) using the Rectangular tool. Note that the measurement is conduced only in the hair cell layer, and that the boundary between the MZ and the TZ is defined as the point where the epidermal cell length in the TZ is twice of that in the MZ.
  3. Register selected regions of interest (ROIs) in the ROI Manager.
  4. Measure the GFP fluorescence intensity for registered ROIs.
Copyright: © 2025 The Author(s); This is an open-access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Takahashi, N and Umeda, M(2025). Measurement of the fluorescence intensity of TCSn:GFP in the Arabidopsis root epidermis. Bio-protocol Preprint. DOI: 10.21769/p2787.
  2. Takahashi, N., Inagaki, S., Nishimura, K., Sakakibara, H., Antoniadi, I., Karady, M., Ljung, K. and Umeda, M.(2021). Alterations in hormonal signals spatially coordinate distinct responses to DNA double-strand breaks in Arabidopsis roots . Science Advances 7(25). DOI: 10.1126/sciadv.abg0993

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