Protocols shared by authors via RaP service
Journal
PBMC flow cytometry
Authors: Eula Graciele Amorim Neves, Walderez Ornelas Dutra and Maria Bellio
Date: Mar 28, 2025，Views：611
Original protocol used in research article：
Cytotoxic CD4+ T cells driven by T-cell intrinsic IL-18R/MyD88 signaling predominantly infiltrate Trypanosoma cruzi-infected hearts
eLife, ; DOI: 10.7554/eLife.74636
Alizarin red staining and imaging of zebrafish larvae and adults
Authors: Sophie Debaenst and Andy Willaert
Date: Mar 25, 2025，Views：484
Original protocol used in research article：
Crispant analysis in zebrafish as a tool for rapid functional screening of disease-causing genes for bone fragility
eLife, Jan 16, 2025; DOI: 10.7554/eLife.100060
Conformational stability assay and immunoblotting
Authors: Selene Seoyun Lee, Livia Civitelli and Laura Parkkinen
Date: Mar 20, 2025，Views：329
Original protocol used in research article：
Brain-derived and in vitro-seeded alpha-synuclein fibrils exhibit distinct biophysical profiles
eLife, Nov 25, 2024; DOI: 10.7554/eLife.92775
Antibodies
Authors: Marcus Buggert, André Perez-Potti and Takuya Sekine
Date: Mar 14, 2025，Views：323
Original protocol used in research article：
TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918
Neural differentiation assay
Authors: Zhiguo Zhang and Xiaowei Xu
Date: Mar 9, 2025，Views：486
Original protocol used in research article：
Mcm2 promotes stem cell differentiation via its ability to bind H3-H4
eLife, Nov 10, 2022; DOI: 10.7554/eLife.80917
Single-cell RNA-seq
Authors: Kenneth D. Birnbaum and Carlos Ortiz-Ramírez
Date: Feb 27, 2025，Views：46
Original protocol used in research article：
Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327
Kim-1 targeting ability
Authors: Xuefang Lou, Saiping Jiang and Yongzhong Du
Date: Feb 26, 2025，Views：372
Original protocol used in research article：
ROS-responsive chitosan-SS31 prodrug for AKI therapy via rapid distribution in the kidney and long-term retention in the renal tubule
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abb7422
PRPS activity assay
Authors: Huan-Huan Hu, Guang-Ming Lu and Ji-Long Liu
Date: Feb 20, 2025，Views：457
Original protocol used in research article：
Filamentation modulates allosteric regulation of PRPS
eLife, ; DOI: 10.7554/eLife.79552
Myeloid progenitor cell differentiation and microglial cell maturation
Authors: Wenxin Ma, Wai T Wong and Wei Li
Date: Feb 18, 2025，Views：412
Original protocol used in research article：
Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia
eLife, Nov 8, 2024; DOI: 10.7554/eLife.90695
DNA Fluorescence in situ hybridisation (FISH)
Authors: Karin Purshouse and Wendy A Bickmore
Date: Feb 17, 2025，Views：550
Original protocol used in research article：
Oncogene expression from extrachromosomal DNA is driven by copy number amplification and does not require spatial clustering in glioblastoma stem cells
eLife, Dec 7, 2022; DOI: 10.7554/eLife.80207
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