Protocols shared by authors via RaP service
Journal

Hierarchical cell type annotation based on scANVI
Authors: Sheng Liu, Zhichao Miao and Yin Huang
Date: Feb 24, 2026，Views：403
Original protocol used in research article：
A brain cell atlas integrating single-cell transcriptomes across human brain regions
Nat Med, Aug 2, 2024; DOI: 10.1038/s41591-024-03150-z

Electroconvulsive stimulation (ECS) procedure
Authors: Eri Segi-Nishida and Katsunori Kobayashi
Date: Feb 24, 2026，Views：319
Original protocol used in research article：
Rapid and stable changes in maturation-related phenotypes of the adult hippocampal neurons by electroconvulsive treatment
Molecular Brain, Mar 2, 2017; DOI: 10.1186/s13041-017-0288-9

Group-based Minimum Spanning Tree Correlation Network
Authors: Camilo A. Espinosa and Nima Aghaeepour
Date: Feb 18, 2026，Views：368
Original protocol used in research article：
Multiomic signals associated with maternal epidemiological factors contributing to preterm birth in low- and middle-income countries
Science Advances, May 24, 2023; DOI: 10.1126/sciadv.ade7692

Tissue histology and immunofluorescence
Authors: Kaylee M Wells and Catherine D McCusker
Date: Feb 10, 2026，Views：440
Original protocol used in research article：
Neural control of growth and size in the axolotl limb regenerate
eLife, Nov 15, 2021; DOI: 10.7554/eLife.68584

2.7. Immunofluorescence Assay to Detect TiLV in the RHTiB Cell Line
Authors: Piyathip Setthawong, Jidapa Yamkasem and Win Surachetpong
Date: Feb 10, 2026，Views：21
Original protocol used in research article：
Development of IgY-Based Passive Immunization Against Tilapia Lake Virus: Development and In Vitro Neutralization Assays
Viruses, Mar 20, 2025; DOI: 10.3390/v17030448
Preparation of acute brain slice for ex vivo time-lapse imaging
Authors: Sunitha Subhramanian and Ilia V Baskakov
Date: Feb 5, 2026，Views：627
Original protocol used in research article：
Dissecting surveying behavior of reactive microglia under chronic neurodegeneration
eLife, Jan 8, 2026; DOI: 10.7554/eLife.107650
Determination of BV2 Microglial Phagocytic Activity (Fluorescent Microspheres / FluoSpheres Assay; Flow Cytometry/Microscopy)
Authors: Marta Matuszewska, Magdalena Cieślik, Anna Wilkaniec and Grzegorz A. Czapski
Date: Feb 5, 2026，Views：713
Original protocol used in research article：
The Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins Protects Against Microglia-Mediated Neuronal Loss In Vitro
Biomolecules, Apr 4, 2025; DOI: 10.3390/biom15040528
Production and usage of VSV-G pseudotyped lentiviruses
Authors: Soham P. Chowdhury, Diego Acosta-Alvear and Francesca Zappa
Date: Jan 31, 2026，Views：573
Original protocol used in research article：
Baseline unfolded protein response signaling adjusts the timing of the mammalian cell cycle
Molecular Biology of the Cell, May 20, 2024; DOI: 10.1091/mbc.E23-11-0419
Immunofluorescence
Authors: Irene Bolea and Albert Quintana
Date: Jan 28, 2026，Views：434
Original protocol used in research article：
Defined neuronal populations drive fatal phenotype in a mouse model of Leigh syndrome
eLife, Aug 12, 2019; DOI: 10.7554/eLife.47163
Co-immunoprecipitation (co-IP) Following SUMOylation/Ubiquitination Perturbation
Authors: Seung Han Son and Chul Geun Kim
Date: Jan 26, 2026，Views：557
Original protocol used in research article：
SUMOylation-mediated PSME3-20S proteasomal degradation of transcription factor CP2c is crucial for cell cycle progression
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.add4969
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