Protocols shared by authors via RaP service
Journal
Immunofluorescence Protocol for HNF4α (P1 and P2 isoforms)
Authors: Karthikeyani Chellappa, Poonamjot Deol and Frances M Sladek
Date: Jan 12, 2024，Views：1353
Original protocol used in research article：
Opposing roles of nuclear receptor HNF4α isoforms in colitis and colitis-associated colon cancer
eLife, May 11, 2016; DOI: 10.7554/eLife.10903
Estimation of the free energy of the Arg381-Asp410 salt bridge
Authors: Yingjie Wang, Gianluigi Veglia, Dongping Zhong and Jiali Gao
Date: Jan 11, 2024，Views：7
Original protocol used in research article：
Activation mechanism of Drosophila cryptochrome through an allosteric switch
Science Advances, Jun 17, 2021; DOI: 10.1126/sciadv.abg3815
In situ hybridizations
Author: Shelby A Blythe
Date: Jan 9, 2024，Views：1526
Original protocol used in research article：
Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network
eLife, Apr 29, 2020; DOI: 10.7554/eLife.53916
Glycerol gradient sedimentation and trichloroacetic acid precipitation of proteins
Authors: Raquel Crespo and Tokameh Mahmoudi
Date: Jan 9, 2024，Views：1609
Original protocol used in research article：
Gliotoxin identified from a screen of fungal metabolites disrupts 7SK snRNP releases P-TEFb and reverses HIV-1 latency
Science Advances, Aug 11, 2020; DOI: 10.1126/sciadv.aba6617
4.9. Measurement of F-Actin/G-Actin Ratio
Authors: Wei-Lun Liu, Meng-Hsuan Cheng, Inn-Wen Chong and Po-Len Liu
Date: Jan 4, 2024，Views：3
Original protocol used in research article：
High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1
International Journal of Molecular Sciences, Mar 31, 2021; DOI: 10.3390/ijms22073628
Reaching steady state in simulations
Authors: Jeff Gore and Jiliang Hu
Date: Dec 22, 2023，Views：6
Original protocol used in research article：
Emergent phases of ecological diversity and dynamics mapped in microcosms
Science, Oct 6, 2022; DOI: 10.1126/science.abm7841
Bacterial strains
Authors: Zilu Qu, Lei Yin, Xiao-Lian Zhang and Zilu Qu
Date: Dec 21, 2023，Views：3
Original protocol used in research article：
Mycobacterial EST12 activates a RACK1–NLRP3–gasdermin D pyroptosis–IL-1β immune pathway
Science Advances, Oct 22, 2020; DOI: 10.1126/sciadv.aba4733
RNA isolation and qPCR
Authors: Raymundo D Hernandez and Michelle L Olsen
Date: Dec 20, 2023，Views：2225
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
Single-Cell TCR Sequencing
Authors: Bernardo S. Reis and Daniel Mucida
Date: Dec 19, 2023，Views：2208
Original protocol used in research article：
TCR-Vγδ usage distinguishes protumor from antitumor intestinal γδ T cell subsets
Science, Jul 14, 2022; DOI: 10.1126/science.abj8695
IL-2/JES6-1 and IL-2/S4B6 complexes
Author: Marek Kovar
Date: Dec 19, 2023，Views：2238
Original protocol used in research article：
IL-2/JES6-1 mAb complexes dramatically increase sensitivity to LPS through IFN-γ production by CD25+Foxp3- T cells
eLife, Dec 21, 2021; DOI: 10.7554/eLife.62432
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