Protocols shared by authors via RaP service
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2.2. Cell culture and transfection
Author: Flavia Carla Meotti
Date: Apr 17, 2026，Views：196
Original protocol used in research article：
Unraveling the effects of uric acid on endothelial cells: A global proteomic study
Redox Biology, Apr 1, 2025; DOI: 10.1016/j.redox.2025.103625

Detailed protocol for Johnsen scoring in testicular histopathology
Author: Hideyuki Kobayashi
Date: Apr 15, 2026，Views：1283
Original protocol used in research article：
A method for utilizing automated machine learning for histopathological classification of testis based on Johnsen scores
Scientific Reports, May 10, 2021; DOI: 10.1038/s41598-021-89369-z

Detailed protocol for generating closed-, open-, and inactivated-state hERG channel models using ColabFold with tailored structural templates
Authors: Khoa Ngo, Pei-Chi Yang, Vladimir Yarov-Yarovoy, Colleen E Clancy and Igor Vorobyov
Date: Apr 14, 2026，Views：432
Original protocol used in research article：
Harnessing AlphaFold to reveal hERG channel conformational state secrets
eLife, Jul 14, 2025; DOI: 10.7554/eLife.104901

Primary human monocyte and macrophage culture
Authors: Jessica Tieng and Justin J-L Wong
Date: Apr 13, 2026，Views：175
Original protocol used in research article：
Macrophage development and activation involve coordinated intron retention in key inflammatory regulators
Nucleic Acids Research, Jan 1, 2020; DOI: 10.1093/nar/gkaa435

Fluorescent in situ hybridization (FISH) and TEM correlation
Authors: Jean-Marie Volland, Jean-Marie Volland and Shailesh V Volland
Date: Apr 12, 2026，Views：55
Original protocol used in research article：
A centimeter-long bacterium with DNA contained in metabolically active, membrane-bound organelles
Science, Jun 23, 2022; DOI: 10.1126/science.abb3634

Expression and Purification of M. smegmatis DnaN (β subunit) 
Authors: Sophia Gessner, Digby F Warner and Joana Abreu Santos
Date: Apr 10, 2026，Views：266
Original protocol used in research article：
Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome
eLife, Aug 2, 2023; DOI: 10.7554/eLife.75628

Yeast one-hybrid assay using the pLacZi2u/pB42AD system
Author: Huang Liyu
Date: Apr 8, 2026，Views：353
Original protocol used in research article：
Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice
Nature Communications, Feb 5, 2020; DOI: 10.1038/s41467-019-14264-1

Mouse husbandry and tissue collection
Author: Oliver J Rando
Date: Apr 6, 2026，Views：329
Original protocol used in research article：
Deep sequencing of yeast and mouse tRNAs and tRNA fragments using OTTR
eLife, Apr 25, 2025; DOI: 10.7554/eLife.77616

Surgical removal of iBAT
Authors: Yuna Izumi-Mishima and Hiroshi Sakaue
Date: Mar 31, 2026，Views：331
Original protocol used in research article：
Brown adipose tissue and skeletal muscle coordinately contribute to thermogenesis in mice
eLife, Oct 27, 2025; DOI: 10.7554/eLife.99982

Cell dissociation (Yale)
Author: Micha Sam Brickman Raredon
Date: Mar 30, 2026，Views：274
Original protocol used in research article：
Single-cell connectomic analysis of adult mammalian lungs
Science Advances, Dec 3, 2019; DOI: 10.1126/sciadv.aaw3851
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