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Preprint

Flow cytometric analysis

AR Ambre Riba
MH Mathias W. Hornef

Last updated date: Dec 17, 2025 Views: 33 Forks: 0

original An abbreviated version of this protocol was published in Science Translational Medicine in Oct, 2020
Disturbed gut microbiota and bile homeostasis in Giardia-infected mice contributes to metabolic dysregulation and growth impairment
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Dear Zhen Yuan, 

We have prepared a layout that explains the gating strategy for the analysis of the immune cells in the paper that you refer to in detail. Details on the reagents are indicated in the Material and Methods section of the Manuscript. I hope you find the information helpful, 

Best wishes, 

Mathias Hornef 


 

Related files

pdf DC mast-Layout-1.pdf download
pdf LP Monomacs-Layout-1.pdf download
Copyright: © 2025 The Author(s); This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Riba, A and Hornef, M(2025). Flow cytometric analysis. Bio-protocol Preprint. bio-protocol.org/prep2882.
  2. Riba, A., Hassani, K., Walker, A., Best, N. V., Zeschwitz, D. V., Anslinger, T., Sillner, N., Rosenhain, S., Eibach, D., Maiga-Ascofaré, O., Rolle-Kampczyk, U., Basic, M., Binz, A., Mocek, S., Sodeik, B., Bauerfeind, R., Mohs, A., Trautwein, C., Kiessling, F., May, J., Klingenspor, M., Gremse, F., Schmitt-Kopplin, P., Bleich, A., Torow, N., Bergen, M. V. and Hornef, M. W.(2020). Disturbed gut microbiota and bile homeostasis in Giardia-infected mice contributes to metabolic dysregulation and growth impairment . Science Translational Medicine 12(565). DOI: 10.1126/scitranslmed.aay7019

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