Protocols shared by authors via RaP service
Journal
Lysosome isolation and analysis
Authors: Yi Xu and Xiaolu Yang
Date: Sep 1, 2020，Views：2306
Original protocol used in research article：
Chaperone-mediated autophagy regulates the pluripotency of embryonic stem cells
Science, Jul 23, 2020; DOI: 10.1126/science.abb4467
Mt-mKeima-based mitophagy assay with FACS
Author: Koji Yamano
Date: Aug 31, 2020，Views：2083
Original protocol used in research article：
Endosomal Rab cycles regulate Parkin-mediated mitophagy
eLife, Jan 23, 2018; DOI: 10.7554/eLife.31326
Isolation of malaria hypnozoites and developing liver stage parasites by Flow Cytometric Cell sorting
Authors: Annemarie Voorberg - van der Wel, Sam O Hofman and Clemens H M Kocken
Date: Aug 31, 2020，Views：1184
Original protocol used in research article：
A comparative transcriptomic analysis of replicating and dormant liver stages of the relapsing malaria parasite Plasmodium cynomolgi
eLife, Dec 7, 2017; DOI: 10.7554/eLife.29605
MBD7 ChIP
Authors: Dongming Li, Xuemei Chen and Julie A Law
Date: Aug 31, 2020，Views：913
Original protocol used in research article：
The MBD7 complex promotes expression of methylated transgenes without significantly altering their methylation status
eLife, Apr 28, 2017; DOI: 10.7554/eLife.19893
Biodegradation of PLGA-MPs
Authors: Xueguang Lu and Ana Jaklenec
Date: Aug 28, 2020，Views：929
Original protocol used in research article：
Engineered PLGA microparticles for long-term pulsatile release of STING agonist for cancer immunotherapy
Science Translational Medicine, Aug 11, 2020; DOI: 10.1126/scitranslmed.aaz6606
RT-LAMP assay
Authors: Viet Loan Dao Thi, Michael Knop and Simon Anders
Date: Aug 28, 2020，Views：1195
Original protocol used in research article：
A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Science Translational Medicine, Aug 12, 2020; DOI: 10.1126/scitranslmed.abc7075
S9.6 Slot blotting
Authors: Karlene A Cimprich and Madzia P Crossley
Date: Aug 27, 2020，Views：1620
Original protocol used in research article：
Co-transcriptional R-loops are the main cause of estrogen-induced DNA damage
eLife, Aug 23, 2016; DOI: 10.7554/eLife.17548
Measurement of transcriptional drift and drift-variance
Author: Michael Petrascheck
Date: Aug 27, 2020，Views：962
Original protocol used in research article：
Suppression of transcriptional drift extends C. elegans lifespan by postponing the onset of mortality
eLife, Dec 1, 2015; DOI: 10.7554/eLife.08833
Recombinant oligonucleosomes
Authors: Fangfang Jiao and Rui Guo
Date: Aug 27, 2020，Views：795
Original protocol used in research article：
RACK7 recognizes H3.3G34R mutation to suppress expression of MHC class II complex components and their delivery pathway in pediatric glioblastoma
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba2113
PCR-RFLP of G1 and G2 mutations in the human Apolipoprotein-L1 (APOL1) gene
Author: Anneli Cooper
Date: Aug 25, 2020，Views：1370
Original protocol used in research article：
APOL1 renal risk variants have contrasting resistance and susceptibility associations with African trypanosomiasis
eLife, May 24, 2017; DOI: 10.7554/eLife.25461
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.