Protocols shared by authors via RaP service
Journal
16S ribosomal RNA gene sequencing
Authors: Bingwei Wang, Lue Dai and Shuo Yang
Date: Sep 21, 2020，Views：853
Original protocol used in research article：
Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation
Science Advances, May 20, 2020; DOI: 10.1126/sciadv.aaz6717
Immunological analyses (IF on Paraffin Sections)
Author: Nainoa Richardson
Date: Sep 21, 2020，Views：850
Original protocol used in research article：
Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals
eLife, May 26, 2020; DOI: 10.7554/eLife.53972
Single-cell RNA-seq and RAR expression in fetal germ cells
Author: Chloé MAYERE
Date: Sep 21, 2020，Views：814
Original protocol used in research article：
Meiosis occurs normally in the fetal ovary of mice lacking all retinoic acid receptors
Science Advances, May 22, 2020; DOI: 10.1126/sciadv.aaz1139
Neutral comet assay
Author: Dongyi Xu
Date: Sep 19, 2020，Views：914
Original protocol used in research article：
53BP1 and BRCA1 control pathway choice for stalled replication restart
eLife, Nov 6, 2017; DOI: 10.7554/eLife.30523
S1 nuclease fiber assay
Authors: Sumeet Nayak and Sharon B. Cantor
Date: Sep 18, 2020，Views：1784
Original protocol used in research article：
Inhibition of the translesion synthesis polymerase REV1 exploits replication gaps as a cancer vulnerability
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz7808
Skin-penetrating IL-RNA complexes
Author: Abhirup Mandal
Date: Sep 18, 2020，Views：1006
Original protocol used in research article：
Treatment of psoriasis with NFKBIZ siRNA using topical ionic liquid formulations
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.abb6049
Statistics
Author: Carlos Guijas
Date: Sep 18, 2020，Views：863
Original protocol used in research article：
Metabolic adaptation to calorie restriction
Science Signaling, Sep 8, 2020; DOI: 10.1126/scisignal.abb2490
Immunostaining of fish scales
Author: Valerie Wittamer
Date: Sep 18, 2020，Views：920
Original protocol used in research article：
Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes
eLife, May 5, 2020; DOI: 10.7554/eLife.53403
Immunofluorescence
Authors: Ruihua Ma and Beatriz Sosa-Pineda
Date: Sep 17, 2020，Views：1153
Original protocol used in research article：
Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts
eLife, Mar 10, 2020; DOI: 10.7554/eLife.46206
RNAscope in situ hybridization
Author: Glenda Comai
Date: Sep 16, 2020，Views：1937
Original protocol used in research article：
A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
eLife, Sep 19, 2019; DOI: 10.7554/eLife.47460
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