Protocols shared by authors via RaP service
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Yeast strains and growth media
Authors: Abbas Jariani, Lieselotte Vermeersch and Kevin J Verstrepen
Date: Sep 4, 2020，Views：1136
Original protocol used in research article：
A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast
eLife, May 18, 2020; DOI: 10.7554/eLife.55320
scRNA-seq using the 10x Genomics platform
Authors: Abbas Jariani, Lieselotte Vermeersch and Kevin J Verstrepen
Date: Sep 4, 2020，Views：1581
Original protocol used in research article：
A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast
eLife, May 18, 2020; DOI: 10.7554/eLife.55320
Zebrafish genotyping
Authors: Kelly R Monk and Xianhua Piao
Date: Sep 3, 2020，Views：908
Original protocol used in research article：
Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells
eLife, May 29, 2018; DOI: 10.7554/eLife.33385
Neonatal transverse sinus injections for whole-brain gene expression
Authors: Ali S Hamodi and Michael Crair
Date: Sep 2, 2020，Views：3314
Original protocol used in research article：
Transverse sinus injections drive robust whole-brain expression of transgenes
eLife, May 18, 2020; DOI: 10.7554/eLife.53639
Pair-wise phase consistency for LFP-LFP phase synchrony
Author: Lu Zhang
Date: Sep 2, 2020，Views：1964
Original protocol used in research article：
Sub-second dynamics of theta-gamma coupling in hippocampal CA1
eLife, Jul 29, 2019; DOI: 10.7554/eLife.44320
Subcellular fractionation and immunoblotting
Author: Miriam Braunstein
Date: Sep 1, 2020，Views：1164
Original protocol used in research article：
Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway
eLife, Jan 3, 2019; DOI: 10.7554/eLife.40063
Culture filtrate protein preparation
Author: Miriam Braunstein
Date: Sep 1, 2020，Views：1149
Original protocol used in research article：
Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway
eLife, Jan 3, 2019; DOI: 10.7554/eLife.40063
Lysosome isolation and analysis
Authors: Yi Xu and Xiaolu Yang
Date: Sep 1, 2020，Views：2418
Original protocol used in research article：
Chaperone-mediated autophagy regulates the pluripotency of embryonic stem cells
Science, Jul 23, 2020; DOI: 10.1126/science.abb4467
Mt-mKeima-based mitophagy assay with FACS
Author: Koji Yamano
Date: Aug 31, 2020，Views：2157
Original protocol used in research article：
Endosomal Rab cycles regulate Parkin-mediated mitophagy
eLife, Jan 23, 2018; DOI: 10.7554/eLife.31326
Isolation of malaria hypnozoites and developing liver stage parasites by Flow Cytometric Cell sorting
Authors: Annemarie Voorberg - van der Wel, Sam O Hofman and Clemens H M Kocken
Date: Aug 31, 2020，Views：1224
Original protocol used in research article：
A comparative transcriptomic analysis of replicating and dormant liver stages of the relapsing malaria parasite Plasmodium cynomolgi
eLife, Dec 7, 2017; DOI: 10.7554/eLife.29605
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