Protocols shared by authors via RaP service
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ML algorithms and performance measures
Author: Juliana Bernardes
Date: Aug 25, 2020，Views：838
Original protocol used in research article：
Structure-specific DNA recombination sites: Design validation and machine learning–based refinement
Science Advances, Jul 23, 2020; DOI: 10.1126/sciadv.aay2922
Immunostaining
Author: Junhua Yang
Date: Aug 24, 2020，Views：925
Original protocol used in research article：
Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP
eLife, Apr 12, 2016; DOI: 10.7554/eLife.15043
Encapsulated Rapamycin Feeding Protocol
Authors: Matt Kaeberlein and Jonathan An
Date: Aug 24, 2020，Views：1486
Original protocol used in research article：
Rapamycin rejuvenates oral health in aging mice
eLife, Apr 28, 2020; DOI: 10.7554/eLife.54318
Signal pathway investigation
Authors: Funeng Xu and Shaobing Zhou
Date: Aug 23, 2020，Views：802
Original protocol used in research article：
Development of a pH-responsive polymersome inducing endoplasmic reticulum stress and autophagy blockade
Science Advances, Jul 30, 2020; DOI: 10.1126/sciadv.abb8725
Measurement of mtDNA copy number and quantification of heteroplasmy
Author: Zong-Heng Wang
Date: Aug 21, 2020，Views：1211
Original protocol used in research article：
Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila
eLife, Oct 15, 2019; DOI: 10.7554/eLife.49309
GST pull-down assay
Author: Shen Wang
Date: Aug 21, 2020，Views：1200
Original protocol used in research article：
Synaptotagmin-1 C2B domain interacts simultaneously with SNAREs and membranes to promote membrane fusion
eLife, Apr 15, 2016; DOI: 10.7554/eLife.14211
Western blotting and immunoprecipitation
Authors: Hozaifa Metwallly and Tadamitsu Kishimoto
Date: Aug 20, 2020，Views：1577
Original protocol used in research article：
Noncanonical STAT1 phosphorylation expands its transcriptional activity into promoting LPS-induced IL-6 and IL-12p40 production
Science Signaling, Mar 23, 2020; DOI: 10.1126/scisignal.aay0574
Design of Extended Field of View Light Field Microscopy for Whole Brain Functional Imaging in Larval Zebrafish
Authors: LIN CONG, Lu Bai and Kai Wang
Date: Aug 20, 2020，Views：1365
Original protocol used in research article：
Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
eLife, Sep 20, 2017; DOI: 10.7554/eLife.28158
Extraction and purification of the photosynthetic ACIII from R. castenholzii
Authors: Yueyong Xin and Xiaoling Xu
Date: Aug 18, 2020，Views：843
Original protocol used in research article：
Cryo-EM structures of the air-oxidized and dithionite-reduced photosynthetic alternative complex III from Roseiflexus castenholzii
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aba2739
Acute extracellular electrophysiology in mouse primary visual cortex
Authors: Arbora Resulaj, Sarah Ruediger, Shawn R Olsen and Massimo Scanziani
Date: Aug 18, 2020，Views：1151
Original protocol used in research article：
First spikes in visual cortex enable perceptual discrimination
eLife, Apr 16, 2018; DOI: 10.7554/eLife.34044
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