Protocols shared by authors via RaP service
Journal
Cryo–electron tomography
Authors: M. A. Benhaim, K. K. Lee and Vidya Mangala Prasad
Date: Sep 15, 2020，Views：856
Original protocol used in research article：
Structural monitoring of a transient intermediate in the hemagglutinin fusion machinery on influenza virions
Science Advances, Apr 28, 2020; DOI: 10.1126/sciadv.aaz8822
Exosome isolation, Subcellular fractionation, and TCA precipitation
Author: Karoliina Stefanius
Date: Sep 14, 2020，Views：1257
Original protocol used in research article：
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
Quantification of NaV1.1 Protein in Mouse Brains using Meso Scale Discovery (MSD) Method
Authors: Zhou Han and Gene Liau
Date: Sep 14, 2020，Views：1056
Original protocol used in research article：
Antisense oligonucleotides increase Scn1a expression and reduce seizures and SUDEP incidence in a mouse model of Dravet syndrome
Science Translational Medicine, Aug 26, 2020; DOI: 10.1126/scitranslmed.aaz6100
NK stimulation assay
Author: Sytse Jan Piersma
Date: Sep 14, 2020，Views：960
Original protocol used in research article：
A herpesvirus encoded Qa-1 mimic inhibits natural killer cell cytotoxicity through CD94/NKG2A receptor engagement
eLife, Dec 21, 2018; DOI: 10.7554/eLife.38667
Expression and Purification of the Human Cation-Chloride Cotransporter KCC1 from HEK293F cells for Structural Studies
Authors: Si Liu and Jiangtao Guo
Date: Sep 14, 2020，Views：1266
Original protocol used in research article：
Cryo-EM structures of the human cation-chloride cotransporter KCC1
Science, Oct 25, 2019; DOI: 10.1126/science.aay3129
Human monocyte-derived dendritic cell (moDC) differentiation and suppression of moDCs by Tregs
Authors: Qing Jessica Huang and Megan K. Levings
Date: Sep 13, 2020，Views：1103
Original protocol used in research article：
Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells
Science Translational Medicine, Aug 19, 2020; DOI: 10.1126/scitranslmed.aaz3866
IgH recombination
Author: Masaki Miyazaki
Date: Sep 13, 2020，Views：899
Original protocol used in research article：
The transcription factor E2A activates multiple enhancers that drive Rag expression in developing T and B cells
Science Immunology, Sep 3, 2020; DOI: 10.1126/sciimmunol.abb1455
Synthesis and characterization of CpG-PP, CpG-PM, and CpG-P-ss-M
Authors: Huile Gao and Lin Qin
Date: Sep 10, 2020，Views：989
Original protocol used in research article：
A tumor-to-lymph procedure navigated versatile gel system for combinatorial therapy against tumor recurrence and metastasis
Science Advances, Sep 3, 2020; DOI: 10.1126/sciadv.abb3116
Quantitative RT-PCR analysis
Author: Katherine A Donovan
Date: Sep 10, 2020，Views：1048
Original protocol used in research article：
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
eLife, Aug 1, 2018; DOI: 10.7554/eLife.38430
Detection and quantitation of NAD+- and NADH-capped RNA in vivo: hybridization with a radiolabeled oligodeoxyribonucleotide probe
Author: Jeremy G Bird
Date: Sep 10, 2020，Views：1011
Original protocol used in research article：
Highly efficient 5' capping of mitochondrial RNA with NAD+ and NADH by yeast and human mitochondrial RNA polymerase
eLife, Dec 12, 2018; DOI: 10.7554/eLife.42179
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.