Protocols shared by authors via RaP service
Journal
C-RNA Sample Preparation Protocol
Authors: Carlo Randise-Hinchliff and Sarah Munchel
Date: Aug 2, 2020，Views：1063
Original protocol used in research article：
Circulating transcripts in maternal blood reflect a molecular signature of early-onset preeclampsia
Science Translational Medicine, Jun 30, 2020; DOI: 10.1126/scitranslmed.aaz0131
Single-cell intracellular Ca2+ microfluorimetry
Author: Arthur Conigrave
Date: Aug 1, 2020，Views：1045
Original protocol used in research article：
Structural mechanism of ligand activation in human calcium-sensing receptor
eLife, Jul 19, 2016; DOI: 10.7554/eLife.13662
Flow cytometry and cell sorting
Authors: Andreas Weigert and Rajkumar Savai
Date: Aug 1, 2020，Views：1003
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Gene set enrichment analysis (GSEA)
Authors: Alexander J. Tarashansky and Bo Wang
Date: Jul 31, 2020，Views：1057
Original protocol used in research article：
Self-assembling manifolds in single-cell RNA sequencing data
eLife, Sep 16, 2019; DOI: 10.7554/eLife.48994
ATPase assay
Authors: Jesper Levring and Ling Wang
Date: Jul 30, 2020，Views：1257
Original protocol used in research article：
Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451
Intracellular IRF5 Dimerization Assay
Authors: Cherrie D Sherman and Betsy J Barnes
Date: Jul 29, 2020，Views：8085
Original protocol used in research article：
Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aay1057
Construction of transgenic, knockout and knockin lines of drosophila
Author: Juan Huang
Date: Jul 29, 2020，Views：1097
Original protocol used in research article：
Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
eLife, Oct 6, 2017; DOI: 10.7554/eLife.26519
Biochemical and immunohistological characterization of brain tissue
Authors: Stefan F Lichtenthaler and Sabina Tahirovic
Date: Jul 28, 2020，Views：995
Original protocol used in research article：
Fibrillar Aβ triggers microglial proteome alterations and dysfunction in Alzheimer mouse models
eLife, Jun 8, 2020; DOI: 10.7554/eLife.54083
Reverse phase(RP)-high-performance liquid chromatography (HPLC) analysis of globin chains
Authors: Anne Chalumeau, Giacomo Frati and Annarita Miccio
Date: Jul 28, 2020，Views：1182
Original protocol used in research article：
Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype
Science Advances, Feb 12, 2020; DOI: 10.1126/sciadv.aay9392
In vivo biodistribution of AUNPs after PDT
Author: Duo Mao
Date: Jul 28, 2020，Views：1064
Original protocol used in research article：
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712
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