Protocols shared by authors via RaP service
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Zebrafish strains, husbandry, embryo treatments, and morpholinos
Author: Julien Vermot
Date: Aug 5, 2020，Views：1004
Original protocol used in research article：
Mechanically activated piezo channels modulate outflow tract valve development through the Yap1 and Klf2-Notch signaling axis
eLife, Sep 16, 2019; DOI: 10.7554/eLife.44706
Lysosomal exocytosis assay (Hexosaminidase)
Authors: Elisabeth S Butz and Christian Grimm
Date: Aug 5, 2020，Views：3677
Original protocol used in research article：
Selective agonist of TRPML2 reveals direct role in chemokine release from innate immune cells
eLife, Nov 27, 2018; DOI: 10.7554/eLife.39720
Social isolation of ants in queenless, broodless conditions
Author: Adria C LeBoeuf
Date: Aug 5, 2020，Views：1014
Original protocol used in research article：
Oral transfer of chemical cues, growth proteins and hormones in social insects
eLife, Nov 29, 2016; DOI: 10.7554/eLife.20375
Flow cytometry analysis
Author: Stefanie Gryzik
Date: Aug 4, 2020，Views：1022
Original protocol used in research article：
Identification of a super-functional Tfh-like subpopulation in murine lupus by pattern perception
eLife, May 22, 2020; DOI: 10.7554/eLife.53226
KEGG pathway enrichment analysis using clusterProfiler
Authors: Manon Morin, Emily C Pierce and Rachel J Dutton
Date: Aug 4, 2020，Views：1574
Original protocol used in research article：
Changes in the genetic requirements for microbial interactions with increasing community complexity
eLife, Sep 13, 2018; DOI: 10.7554/eLife.37072
Connectomic Analysis of Tissues
Author: Sam Brickman Raredon
Date: Aug 3, 2020，Views：970
Original protocol used in research article：
Single-cell connectomic analysis of adult mammalian lungs
Science Advances, Dec 3, 2019; DOI: 10.1126/sciadv.aaw3851
Immunohistochemistry on whole mount retinas
Author: Claudia Prahst
Date: Aug 3, 2020，Views：1944
Original protocol used in research article：
Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
eLife, Feb 19, 2020; DOI: 10.7554/eLife.49779
C-RNA Sample Preparation Protocol
Authors: Carlo Randise-Hinchliff and Sarah Munchel
Date: Aug 2, 2020，Views：1095
Original protocol used in research article：
Circulating transcripts in maternal blood reflect a molecular signature of early-onset preeclampsia
Science Translational Medicine, Jun 30, 2020; DOI: 10.1126/scitranslmed.aaz0131
Single-cell intracellular Ca2+ microfluorimetry
Author: Arthur Conigrave
Date: Aug 1, 2020，Views：1068
Original protocol used in research article：
Structural mechanism of ligand activation in human calcium-sensing receptor
eLife, Jul 19, 2016; DOI: 10.7554/eLife.13662
Flow cytometry and cell sorting
Authors: Andreas Weigert and Rajkumar Savai
Date: Aug 1, 2020，Views：1020
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
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