Protocols shared by authors via RaP service
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Screening PETAL array with proteomic antigens
Author: Zhaohui Wang
Date: Jul 16, 2020，Views：957
Original protocol used in research article：
An array of 60000 antibodies for proteome-scale antibody generation and target discovery
Science Advances, Mar 10, 2020; DOI: 10.1126/sciadv.aax2271
In vitro release of Ad from NPs in serum
Author: Patrick Couvreur
Date: Jul 15, 2020，Views：948
Original protocol used in research article：
Squalene-based multidrug nanoparticles for improved mitigation of uncontrolled inflammation in rodents
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz5466
Proteinase K protection assay
Authors: Min Zhang and Lei Liu
Date: Jul 15, 2020，Views：3060
Original protocol used in research article：
Translocation of interleukin-1β into a vesicle intermediate in autophagy-mediated secretion
eLife, Nov 2, 2015; DOI: 10.7554/eLife.11205
Fluorescence quenching assay
Author: Mable Lam
Date: Jul 15, 2020，Views：995
Original protocol used in research article：
Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52291
Peptide pulldown with Fc-tagged ECD proteins
Author: Mable Lam
Date: Jul 14, 2020，Views：999
Original protocol used in research article：
Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52291
Kcr peptide enrichment
Authors: Zhongyi Cheng and Jing Liang
Date: Jul 14, 2020，Views：996
Original protocol used in research article：
Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination–mediated DNA repair
Science Advances, Mar 12, 2020; DOI: 10.1126/sciadv.aay4697
Lectin staining
Author: Katarina Valoskova
Date: Jul 14, 2020，Views：1403
Original protocol used in research article：
A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion
eLife, Mar 26, 2019; DOI: 10.7554/eLife.41801
Immunofluorescence staining – quantification of activated-caspase 3-positive cells in brain slices:
Authors: Oriane Blanquie, Heiko J Luhmann, Jenq-Wei Yang, Werner Kilb, Salim Sharopov and Anne Sinning
Date: Jul 14, 2020，Views：1337
Original protocol used in research article：
Electrical activity controls area-specific expression of neuronal apoptosis in the mouse developing cerebral cortex
eLife, Aug 21, 2017; DOI: 10.7554/eLife.27696
Lung tumor volume measurements from X-ray micro-computed tomography (μCT) scans
Authors: Caroline Contat and Etienne Meylan
Date: Jul 14, 2020，Views：1173
Original protocol used in research article：
Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth
eLife, Jun 23, 2020; DOI: 10.7554/eLife.53618
2-NBDG uptake
Authors: Kenneth Kin Lam Wong and Esther M Verheyen
Date: Jul 13, 2020，Views：1190
Original protocol used in research article：
A positive feedback loop between Myc and aerobic glycolysis sustains tumor growth in a Drosophila tumor model
eLife, Jul 1, 2019; DOI: 10.7554/eLife.46315
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