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Last updated date: Sep 18, 2020 Views: 826 Forks: 0
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Network analysis was performed with the R package igraph by considering only pairs of metabolites with a Pearson correlation coefficient above 0.6. Outliers were not removed. Ten mice were used for each group, except for AL at 22°C (nine mice). All 90 plasma metabolites and 37 hypothalamic metabolites were used to compute the correlations [all metabolites found except those hypothalamic metabolites that were measured at 22°C but could not be found at 30°C: PC(32:0), SM(d18:1/17:0), erythro-sphingosine, arachidonoylglycerol, NADP+, and homoarginine]. The number of samples used to calculate the correlation between any two metabolites was the same (39 samples at 22°C and 40 samples at 30°C).
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- Guijas, C(2020). Statistics. Bio-protocol Preprint. bio-protocol.org/prep508.
- Guijas, C., Montenegro-Burke, J. R., Cintron-Colon, R., Domingo-Almenara, X., Sanchez-Alavez, M., Aguirre, C. A., Shankar, K., Majumder, E. L., Billings, E., Conti, B. and Siuzdak, G.(2020). Metabolic adaptation to calorie restriction. Science Signaling 13(648). DOI: 10.1126/scisignal.abb2490
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