Protocols shared by authors via RaP service
Journal
Overexpression and purification of the recombinant β-actin inclusion-body protein
Author: Jakub Drozak
Date: Jul 21, 2020，Views：1078
Original protocol used in research article：
Structural insights into SETD3-mediated histidine methylation on β-actin
eLife, Feb 20, 2019; DOI: 10.7554/eLife.43676
Calculation of residue-residue contact score (RRCS)
Authors: Qingtong Zhou, Ming-Wei Wang and Suwen Zhao
Date: Jul 21, 2020，Views：1549
Original protocol used in research article：
Common activation mechanism of class A GPCRs
eLife, Dec 19, 2019; DOI: 10.7554/eLife.50279
Expression and purification of SpyCas9
Authors: Xinyu Ling and Tao Liu
Date: Jul 20, 2020，Views：1063
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Phagocytosis assay
Authors: Alexandre B Leitão and Élio Sucena
Date: Jul 20, 2020，Views：987
Original protocol used in research article：
Drosophila sessile hemocyte clusters are true hematopoietic tissues that regulate larval blood cell differentiation
eLife, Feb 4, 2015; DOI: 10.7554/eLife.06166
Barcode association of library variants
Authors: Julia M Flynn and Daniel NA Bolon
Date: Jul 20, 2020，Views：1087
Original protocol used in research article：
Comprehensive fitness maps of Hsp90 show widespread environmental dependence
eLife, Mar 4, 2020; DOI: 10.7554/eLife.53810
Probability of acquisition
Author: Thomas Crellen
Date: Jul 20, 2020，Views：1138
Original protocol used in research article：
Transmission dynamics and control of multidrug-resistant Klebsiella pneumoniae in neonates in a developing country
eLife, Dec 3, 2019; DOI: 10.7554/eLife.50468
In vivo efficacy study
Authors: Chad V. Pecot, Alexander V. Kabanov and Natasha Vinod
Date: Jul 17, 2020，Views：925
Original protocol used in research article：
High-capacity poly(2-oxazoline) formulation of TLR 7/8 agonist extends survival in a chemo-insensitive metastatic model of lung adenocarcinoma
Science Advances, Jun 16, 2020; DOI: 10.1126/sciadv.aba5542
Screening PETAL array with proteomic antigens
Author: Zhaohui Wang
Date: Jul 16, 2020，Views：986
Original protocol used in research article：
An array of 60000 antibodies for proteome-scale antibody generation and target discovery
Science Advances, Mar 10, 2020; DOI: 10.1126/sciadv.aax2271
In vitro release of Ad from NPs in serum
Author: Patrick Couvreur
Date: Jul 15, 2020，Views：976
Original protocol used in research article：
Squalene-based multidrug nanoparticles for improved mitigation of uncontrolled inflammation in rodents
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz5466
Proteinase K protection assay
Authors: Min Zhang and Lei Liu
Date: Jul 15, 2020，Views：3127
Original protocol used in research article：
Translocation of interleukin-1β into a vesicle intermediate in autophagy-mediated secretion
eLife, Nov 2, 2015; DOI: 10.7554/eLife.11205
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