Protocols shared by authors via RaP service
Journal
Gene set enrichment analysis (GSEA)
Authors: Alexander J. Tarashansky and Bo Wang
Date: Jul 31, 2020，Views：1118
Original protocol used in research article：
Self-assembling manifolds in single-cell RNA sequencing data
eLife, Sep 16, 2019; DOI: 10.7554/eLife.48994
ATPase assay
Authors: Jesper Levring and Ling Wang
Date: Jul 30, 2020，Views：1310
Original protocol used in research article：
Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451
Intracellular IRF5 Dimerization Assay
Authors: Cherrie D Sherman and Betsy J Barnes
Date: Jul 29, 2020，Views：8428
Original protocol used in research article：
Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aay1057
Construction of transgenic, knockout and knockin lines of drosophila
Author: Juan Huang
Date: Jul 29, 2020，Views：1128
Original protocol used in research article：
Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
eLife, Oct 6, 2017; DOI: 10.7554/eLife.26519
Biochemical and immunohistological characterization of brain tissue
Authors: Stefan F Lichtenthaler and Sabina Tahirovic
Date: Jul 28, 2020，Views：1029
Original protocol used in research article：
Fibrillar Aβ triggers microglial proteome alterations and dysfunction in Alzheimer mouse models
eLife, Jun 8, 2020; DOI: 10.7554/eLife.54083
Reverse phase(RP)-high-performance liquid chromatography (HPLC) analysis of globin chains
Authors: Anne Chalumeau, Giacomo Frati and Annarita Miccio
Date: Jul 28, 2020，Views：1254
Original protocol used in research article：
Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype
Science Advances, Feb 12, 2020; DOI: 10.1126/sciadv.aay9392
In vivo biodistribution of AUNPs after PDT
Author: Duo Mao
Date: Jul 28, 2020，Views：1074
Original protocol used in research article：
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712
Insects and UV images
Author: Ryo Futahashi
Date: Jul 27, 2020，Views：1049
Original protocol used in research article：
Molecular basis of wax-based color change and UV reflection in dragonflies
eLife, Jan 15, 2019; DOI: 10.7554/eLife.43045
Immunohistochemistry
Authors: Joel K Elmquist, Alexandre Caron and Charlotte Elizabeth Lee
Date: Jul 27, 2020，Views：1138
Original protocol used in research article：
POMC neurons expressing leptin receptors coordinate metabolic responses to fasting via suppression of leptin levels
eLife, Mar 12, 2018; DOI: 10.7554/eLife.33710
Real-time quantitative PCR
Author: Sunhye Shin
Date: Jul 27, 2020，Views：1180
Original protocol used in research article：
Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
eLife, Jun 19, 2020; DOI: 10.7554/eLife.56189
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