Protocols shared by authors via RaP service
Journal
GST pull-down assay
Author: Shen Wang
Date: Aug 21, 2020，Views：1262
Original protocol used in research article：
Synaptotagmin-1 C2B domain interacts simultaneously with SNAREs and membranes to promote membrane fusion
eLife, Apr 15, 2016; DOI: 10.7554/eLife.14211
Western blotting and immunoprecipitation
Authors: Hozaifa Metwallly and Tadamitsu Kishimoto
Date: Aug 20, 2020，Views：1762
Original protocol used in research article：
Noncanonical STAT1 phosphorylation expands its transcriptional activity into promoting LPS-induced IL-6 and IL-12p40 production
Science Signaling, Mar 23, 2020; DOI: 10.1126/scisignal.aay0574
Design of Extended Field of View Light Field Microscopy for Whole Brain Functional Imaging in Larval Zebrafish
Authors: LIN CONG, Lu Bai and Kai Wang
Date: Aug 20, 2020，Views：1412
Original protocol used in research article：
Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
eLife, Sep 20, 2017; DOI: 10.7554/eLife.28158
Extraction and purification of the photosynthetic ACIII from R. castenholzii
Authors: Yueyong Xin and Xiaoling Xu
Date: Aug 18, 2020，Views：888
Original protocol used in research article：
Cryo-EM structures of the air-oxidized and dithionite-reduced photosynthetic alternative complex III from Roseiflexus castenholzii
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aba2739
Acute extracellular electrophysiology in mouse primary visual cortex
Authors: Arbora Resulaj, Sarah Ruediger, Shawn R Olsen and Massimo Scanziani
Date: Aug 18, 2020，Views：1191
Original protocol used in research article：
First spikes in visual cortex enable perceptual discrimination
eLife, Apr 16, 2018; DOI: 10.7554/eLife.34044
Rubicon ThruPlex DNA-Seq library preparation protocol
Author: Laura A Lavery
Date: Aug 18, 2020，Views：1049
Original protocol used in research article：
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Western blotting
Author: Daniel T Hughes
Date: Aug 18, 2020，Views：939
Original protocol used in research article：
Targeting the kinase insert loop of PERK selectively modulates PERK signaling without systemic toxicity in mice
Science Signaling, Aug 10, 2020; DOI: 10.1126/scisignal.abb4749
Cell culture and treatment
Authors: Hyungsoo Kim and Ze’ev A. Ronai
Date: Aug 17, 2020，Views：1002
Original protocol used in research article：
PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aaz5683
Preparation of SiUNPs, AUNPs, and pAUNPs
Author: Duo Mao
Date: Aug 17, 2020，Views：922
Original protocol used in research article：
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712
In situ hybridization and antibody labeling
Author: Catherine M Drerup
Date: Aug 14, 2020，Views：973
Original protocol used in research article：
Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons
eLife, Apr 17, 2017; DOI: 10.7554/eLife.22234
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.