Protocols shared by authors via RaP service
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Biochemical and immunohistological characterization of brain tissue
Authors: Stefan F Lichtenthaler and Sabina Tahirovic
Date: Jul 28, 2020，Views：962
Original protocol used in research article：
Fibrillar Aβ triggers microglial proteome alterations and dysfunction in Alzheimer mouse models
eLife, Jun 8, 2020; DOI: 10.7554/eLife.54083
Reverse phase(RP)-high-performance liquid chromatography (HPLC) analysis of globin chains
Authors: Anne Chalumeau, Giacomo Frati and Annarita Miccio
Date: Jul 28, 2020，Views：1110
Original protocol used in research article：
Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype
Science Advances, Feb 12, 2020; DOI: 10.1126/sciadv.aay9392
In vivo biodistribution of AUNPs after PDT
Author: Duo Mao
Date: Jul 28, 2020，Views：1038
Original protocol used in research article：
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712
Insects and UV images
Author: Ryo Futahashi
Date: Jul 27, 2020，Views：1005
Original protocol used in research article：
Molecular basis of wax-based color change and UV reflection in dragonflies
eLife, Jan 15, 2019; DOI: 10.7554/eLife.43045
Immunohistochemistry
Authors: Joel K Elmquist, Alexandre Caron and Charlotte Elizabeth Lee
Date: Jul 27, 2020，Views：1087
Original protocol used in research article：
POMC neurons expressing leptin receptors coordinate metabolic responses to fasting via suppression of leptin levels
eLife, Mar 12, 2018; DOI: 10.7554/eLife.33710
Real-time quantitative PCR
Author: Sunhye Shin
Date: Jul 27, 2020，Views：1115
Original protocol used in research article：
Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
eLife, Jun 19, 2020; DOI: 10.7554/eLife.56189
Experiment 1: Effect of central (ICV) administration of senktide on LH release in male and female WT and Kiss1 KO mice with or without the presence of sex steroids.
Author: Chrysanthi Fergani
Date: Jul 27, 2020，Views：948
Original protocol used in research article：
NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice
eLife, Dec 19, 2018; DOI: 10.7554/eLife.40476
Generation of knockout flies
Author: Mohammed A. Khallaf
Date: Jul 27, 2020，Views：1010
Original protocol used in research article：
Mate discrimination among subspecies through a conserved olfactory pathway
Science Advances, Jun 16, 2020; DOI: 10.1126/sciadv.aba5279
Chromatin immunoprecipitation-deep sequencing (ChIP-seq) and ChIP-qPCR
Authors: Dong Fang and Zhiguo Zhang
Date: Jul 26, 2020，Views：3200
Original protocol used in research article：
H3.3K27M mutant proteins reprogram epigenome by sequestering the PRC2 complex to poised enhancers
eLife, Jun 22, 2018; DOI: 10.7554/eLife.36696
Human embryonic lung organoid culture including recovering organoids from matrigel for immunostaining or RNA extraction
Authors: Emma L Rawlins and Dawei Sun
Date: Jul 23, 2020，Views：1298
Original protocol used in research article：
Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
eLife, Jun 30, 2017; DOI: 10.7554/eLife.26575
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