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Heparin-induced filament assembly of tau

WZ Wenjuan Zhang

Last updated date: Sep 28, 2020 Views: 1623 Forks: 0

original An abbreviated version of this protocol was published in eLife in Feb, 2019
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
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Heparin-induced filament assembly of tau

 

1. Heparin (Sigma) was resolved in water into 50mg/ml.

2. Make Heparin master mix as follows:

50mg/ml Heparin

4μl

300mM MOPS, pH7.2

50μl

100mM AEBSF

5μl

Sterile water

41μl

Total:

100μl

 

3. Add 5μl Heparin master mix into 20μl 3ml/ml tau protein.

4. Vortex briefly to mix.

5. Incubate at 37°C for 72 hours.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Zhang, W(2020). Heparin-induced filament assembly of tau. Bio-protocol Preprint. bio-protocol.org/prep524.
  2. Zhang, W., Falcon, B., Murzin, A. G., Fan, J., Crowther, R. A., Goedert, M. and Scheres, S. H.(2019). Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases. eLife. DOI: 10.7554/eLife.43584

Category

Biophysics

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