Protocols shared by authors via RaP service
Journal
Experiment 1: Effect of central (ICV) administration of senktide on LH release in male and female WT and Kiss1 KO mice with or without the presence of sex steroids.
Author: Chrysanthi Fergani
Date: Jul 27, 2020，Views：996
Original protocol used in research article：
NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice
eLife, Dec 19, 2018; DOI: 10.7554/eLife.40476
Generation of knockout flies
Author: Mohammed A. Khallaf
Date: Jul 27, 2020，Views：1051
Original protocol used in research article：
Mate discrimination among subspecies through a conserved olfactory pathway
Science Advances, Jun 16, 2020; DOI: 10.1126/sciadv.aba5279
Chromatin immunoprecipitation-deep sequencing (ChIP-seq) and ChIP-qPCR
Authors: Dong Fang and Zhiguo Zhang
Date: Jul 26, 2020，Views：3403
Original protocol used in research article：
H3.3K27M mutant proteins reprogram epigenome by sequestering the PRC2 complex to poised enhancers
eLife, Jun 22, 2018; DOI: 10.7554/eLife.36696
Human embryonic lung organoid culture including recovering organoids from matrigel for immunostaining or RNA extraction
Authors: Emma L Rawlins and Dawei Sun
Date: Jul 23, 2020，Views：1549
Original protocol used in research article：
Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
eLife, Jun 30, 2017; DOI: 10.7554/eLife.26575
cLTP and cLTD protocols
Author: Okunola Jeyifous
Date: Jul 22, 2020，Views：1693
Original protocol used in research article：
Synaptic activity regulates AMPA receptor trafficking through different recycling pathways
eLife, May 13, 2015; DOI: 10.7554/eLife.06878
Block-bootstrap procedure
Author: Fanny Pouyet
Date: Jul 21, 2020，Views：1346
Original protocol used in research article：
Background selection and biased gene conversion affect more than 95% of the human genome and bias demographic inferences
eLife, Aug 23, 2018; DOI: 10.7554/eLife.36317
In vitro effects of YAP1-targeting drugs
Authors: Tomohiko Maehama, Akira Suzuki and Hirofumi Omori
Date: Jul 21, 2020，Views：924
Original protocol used in research article：
YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma
Science Advances, Mar 17, 2020; DOI: 10.1126/sciadv.aay3324
Overexpression and purification of the recombinant β-actin inclusion-body protein
Author: Jakub Drozak
Date: Jul 21, 2020，Views：1099
Original protocol used in research article：
Structural insights into SETD3-mediated histidine methylation on β-actin
eLife, Feb 20, 2019; DOI: 10.7554/eLife.43676
Calculation of residue-residue contact score (RRCS)
Authors: Qingtong Zhou, Ming-Wei Wang and Suwen Zhao
Date: Jul 21, 2020，Views：1592
Original protocol used in research article：
Common activation mechanism of class A GPCRs
eLife, Dec 19, 2019; DOI: 10.7554/eLife.50279
Expression and purification of SpyCas9
Authors: Xinyu Ling and Tao Liu
Date: Jul 20, 2020，Views：1082
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.