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Preprint

Flow cytometry antibody panel

AB Alexandre Boissonnas
PL Pierre Louis Loyher

Last updated date: Sep 16, 2020 Views: 968 Forks: 0

original An abbreviated version of this protocol was published in eLife in Jul, 2015
Immune surveillance of the lung by migrating tissue monocytes
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Antigen

Clone/Source

Isotype

Fluorochrome

Dilution

CD11b

M1/70 (BD)

Rat IgG2b,   κ

BUV 395 /PercP Cy5.5

1/200

Ly6C

AL-21 (BD)

Rat IgM, κ

APC-H7

1/100

Ly6G

1A8 (BD)

Rat IgG2a, κ

BV421

1/500

NK1.1

PK136 (BD)

Mouse IgG2a,   κ

PE-Cy7

1/100

CD45

30-F11 (BD)

Rat IgG2b, κ

PercP-Cy5.5

1/100

CD11c

HL3 (BD)

Armenian Hamster   IgG1, λ2

BV605

1/100

I-Ab

AF6-120.1

Mouse IgG2a, κ

APC

1/100

CD62L

MEL-14 (BD)

Rat IgG2a, κ

PE

1/100

Siglec-F

E50-2440 (BD

Rat IgG2a, κ

PE-CF594

1/100

CD24

M1/69 (BD)

Rat IgG2b, κ

BV650

1/200

CD103

M290 (BD)

Rat IgG2a, κ

BV786 / PE

1/200

F4/80

BM8 (Biolegend)

Rat IgG2a, κ

A700

1/200

CD115

AFS98 (Biolegend)

Rat IgG2a κ

 

PE-Cy7 / PE

1/100

CD64

X54-5/7.1.1

(Biolegend)

Mouse IgG1 κ

PE-Cy7 / PE

1/500

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Boissonnas, A and Loyher, P(2020). Flow cytometry antibody panel. Bio-protocol Preprint. bio-protocol.org/prep504.
  2. Rodero, M. P., Poupel, L., Loyher, P., Hamon, P., Licata, F., Pessel, C., Hume, D. A., Combadière, C. and Boissonnas, A.(2015). Immune surveillance of the lung by migrating tissue monocytes. eLife. DOI: 10.7554/eLife.07847

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