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Assessment of CpG methylation status by bisulfite sequencing
Last updated date: Oct 23, 2020 Views: 876 Forks: 0
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Assessment of CpG methylation status by bisulfite sequencing
Purified genomic DNA or ChIP DNA from H1 cells and treated by EZ DNA Methylation-Direct Kit (Zymo Research, Irvine, CA) for bisulfite conversion.
After bisulfite conversion, regions of interest were PCR-amplified. The primers were listed in Supplementary file 1E.
PCR products were gel-purified and cloned into a TA vector (Life Technologies).
Individual clones were Sanger sequenced and aligned with the reference sequence.
- Su, Y(2020). Assessment of CpG methylation status by bisulfite sequencing. Bio-protocol Preprint. bio-protocol.org/prep568.
- Hu, S., Wan, J., Su, Y., Song, Q., Zeng, Y., Nguyen, H. N., Shin, J., Cox, E., Rho, H. S., Woodard, C., Xia, S., Liu, S., Lyu, H., Ming, G., Wade, H., Song, H., Qian, J. and Zhu, H.(2013). DNA methylation presents distinct binding sites for human transcription factors. eLife. DOI: 10.7554/eLife.00726
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