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Assessment of CpG methylation status by bisulfite sequencing

YS Yijing Su

Last updated date: Oct 23, 2020 Views: 885 Forks: 0

original An abbreviated version of this protocol was published in eLife in Sep, 2013
DNA methylation presents distinct binding sites for human transcription factors
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Assessment of CpG methylation status by bisulfite sequencing

  1. Purified genomic DNA or ChIP DNA from H1 cells and treated by EZ DNA Methylation-Direct Kit (Zymo Research, Irvine, CA) for bisulfite conversion.

  2. After bisulfite conversion, regions of interest were PCR-amplified. The primers were listed in Supplementary file 1E.

  3. PCR products were gel-purified and cloned into a TA vector (Life Technologies).

  4. Individual clones were Sanger sequenced and aligned with the reference sequence.


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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Su, Y(2020). Assessment of CpG methylation status by bisulfite sequencing. Bio-protocol Preprint. bio-protocol.org/prep568.
  2. Hu, S., Wan, J., Su, Y., Song, Q., Zeng, Y., Nguyen, H. N., Shin, J., Cox, E., Rho, H. S., Woodard, C., Xia, S., Liu, S., Lyu, H., Ming, G., Wade, H., Song, H., Qian, J. and Zhu, H.(2013). DNA methylation presents distinct binding sites for human transcription factors. eLife. DOI: 10.7554/eLife.00726

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