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LC3 lipidation, membrane fractionation, immunodepletion, and immunoblot

LG Liang Ge

Last updated date: Oct 26, 2020 Views: 1048 Forks: 0

original An abbreviated version of this protocol was published in eLife in Nov, 2014
Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Ge, L(2020). LC3 lipidation, membrane fractionation, immunodepletion, and immunoblot. Bio-protocol Preprint. bio-protocol.org/prep572.
  2. Ge, L., Zhang, M. and Schekman, R.(2014). Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment. eLife. DOI: 10.7554/eLife.04135

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