Protocols shared by authors via RaP service
Journal
RdRp assay for DENV4 NS5Pol
Authors: Cheng-Feng Qin and Zhong-Yu Liu
Date: Oct 15, 2020，Views：936
Original protocol used in research article：
Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization
eLife, Oct 1, 2016; DOI: 10.7554/eLife.17636
Bioinformatics analyses
Authors: Karin Hansson and Daniel Bexell
Date: Oct 15, 2020，Views：757
Original protocol used in research article：
Therapeutic targeting of KSP in preclinical models of high-risk neuroblastoma
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aba4434
Retrograde labeling of motor neurons in adult zebrafish using dextran or DiI
Authors: Buntaro Zempo and Fumihito Ono
Date: Oct 14, 2020，Views：861
Original protocol used in research article：
Synaptic silencing of fast muscle is compensated by rewired innervation of slow muscle
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aax8382
Protocol of full article
Author: Ye Duan
Date: Oct 14, 2020，Views：775
Original protocol used in research article：
Engineering essential genes with a “jump board” strategy using CRISPR/Cas9
microPublication Biology, Oct 8, 2020; DOI: 10.17912/micropub.biology.000315
Nestmate recognition assay
Author: Cassondra L Vernier
Date: Oct 14, 2020，Views：873
Original protocol used in research article：
The cuticular hydrocarbon profiles of honey bee workers develop via a socially-modulated innate process
eLife, Feb 5, 2019; DOI: 10.7554/eLife.41855
Animal studies
Author: Debomita Chakraborty
Date: Oct 13, 2020，Views：770
Original protocol used in research article：
Fibroblast growth factor receptor 3 activates a network of profibrotic signaling pathways to promote fibrosis in systemic sclerosis
Science Translational Medicine, Sep 29, 2020; DOI: 10.1126/scitranslmed.aaz5506
Whole mount immunofluorescence microscopy of mouse E7.5 embryo for cilia stain
Author: Katsutoshi Mizuno
Date: Oct 12, 2020，Views：1115
Original protocol used in research article：
Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.aba1195
Codon optimized sequence of human ORC genes
Author: Matt J Jaremko
Date: Oct 12, 2020，Views：919
Original protocol used in research article：
The dynamic nature of the human origin recognition complex revealed through five cryoEM structures
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58622
Chemicals and synthetic DNA
Author: Monica Rosas-Lemus
Date: Oct 12, 2020，Views：801
Original protocol used in research article：
High-resolution structures of the SARS-CoV-2 2′-O-methyltransferase reveal strategies for structure-based inhibitor design
Science Signaling, Sep 28, 2020; DOI: 10.1126/scisignal.abe1202
Fractionation of neuronal cells into soma and neurite fractions
Authors: Raeann Goering and J Matthew Taliaferro
Date: Oct 12, 2020，Views：1344
Original protocol used in research article：
FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences
eLife, Jun 8, 2020; DOI: 10.7554/eLife.52621
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