Protocols shared by authors via RaP service
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Cloning and purification of VapB22, VapC22, and VapBC22
Authors: Krishan Gopal Thakur and Ramandeep Singh
Date: Oct 21, 2020，Views：874
Original protocol used in research article：
VapBC22 toxin-antitoxin system from Mycobacterium tuberculosis is required for pathogenesis and modulation of host immune response
Science Advances, Jun 2, 2020; DOI: 10.1126/sciadv.aba6944
Electrophysiology
Authors: Shangbang Gao and Zhitao Hu
Date: Oct 21, 2020，Views：882
Original protocol used in research article：
Minimum number of synaptic vesicles for the initiation of a single action potential at C. elegans neuromuscular junction
microPublication Biology, Oct 6, 2020; DOI: 10.17912/micropub.biology.000316
Cell preparation for analysis
Author: Alok Kumar
Date: Oct 20, 2020，Views：979
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Quantification of the diversities of V(D)J recombination events for a given CDR3 peptide
Authors: Jian Han and Leonore A Herzenberg
Date: Oct 20, 2020，Views：1040
Original protocol used in research article：
Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
eLife, Sep 30, 2015; DOI: 10.7554/eLife.09083
CDR3 tree map analysis
Authors: Jian Han and Leonore A Herzenberg
Date: Oct 20, 2020，Views：986
Original protocol used in research article：
Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
eLife, Sep 30, 2015; DOI: 10.7554/eLife.09083
Cuticular hydrocarbon extractions and GC analysis
Authors: Cassondra L Vernier, Yehuda Ben-Shahar and Joshua Krupp
Date: Oct 20, 2020，Views：1026
Original protocol used in research article：
The cuticular hydrocarbon profiles of honey bee workers develop via a socially-modulated innate process
eLife, Feb 5, 2019; DOI: 10.7554/eLife.41855
Zymography
Author: Trayambak Pathak
Date: Oct 20, 2020，Views：1137
Original protocol used in research article：
Dichotomous role of the human mitochondrial Na+/Ca2+/Li+ exchanger NCLX in colorectal cancer growth and metastasis
eLife, Sep 11, 2020; DOI: 10.7554/eLife.59686
On-chip sort, 10X library preparation, and RNA sequencing
Authors: Benjamin W Okaty, Susan M Dymecki and Nikita Sturrock
Date: Oct 19, 2020，Views：1090
Original protocol used in research article：
A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons
eLife, Jun 22, 2020; DOI: 10.7554/eLife.55523
Wireless transmitter and tetrode fabrication
Author: Haleh Fotowat
Date: Oct 19, 2020，Views：882
Original protocol used in research article：
Neural activity in a hippocampus-like region of the teleost pallium is associated with active sensing and navigation
eLife, Apr 3, 2019; DOI: 10.7554/eLife.44119
Antisense oligonucleotide application
Author: Jonathan R Roth
Date: Oct 19, 2020，Views：1096
Original protocol used in research article：
Alzheimer’s disease risk gene BIN1 induces Tau-dependent network hyperexcitability
eLife, Jul 13, 2020; DOI: 10.7554/eLife.57354
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