Protocols shared by authors via RaP service
Journal
Immunohistochemistry (IHC)
Author: Daniel Hasche
Date: Oct 1, 2020，Views：1642
Original protocol used in research article：
Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity
eLife, Aug 4, 2020; DOI: 10.7554/eLife.57626
Ovine model of osteochondral defect repair
Author: Frances Henson
Date: Sep 30, 2020，Views：806
Original protocol used in research article：
Agrin induces long-term osteochondral regeneration by supporting repair morphogenesis
Science Translational Medicine, Sep 1, 2020; DOI: 10.1126/scitranslmed.aax9086
Cryo-EM sample preparation
Authors: Byung-Gil Lee and Jan Löwe
Date: Sep 30, 2020，Views：1134
Original protocol used in research article：
Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3
eLife, Sep 15, 2020; DOI: 10.7554/eLife.59560
Immunoprecipitation and GST pull-down
Author: Arnaud Besson
Date: Sep 29, 2020，Views：1046
Original protocol used in research article：
p27Kip1 promotes invadopodia turnover and invasion through the regulation of the PAK1/Cortactin pathway
eLife, Mar 13, 2017; DOI: 10.7554/eLife.22207
Measurement of heat production (thermogenesis) in cells using ERthermAC dye
Authors: Chih-Hao Wang and Yu-Hua Tseng
Date: Sep 29, 2020，Views：1550
Original protocol used in research article：
CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aaz8664
Heparin-induced filament assembly of tau
Author: Wenjuan Zhang
Date: Sep 28, 2020，Views：1601
Original protocol used in research article：
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584
Flow cytometry
Authors: Christian Perez-Shibayama, Cristina Gil-Cruz and Burkhard Ludewig
Date: Sep 24, 2020，Views：937
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
Droplet-based scRNA-seq analysis
Authors: Christian Perez-Shibayama, Mechthild Luedge and Burkhard Ludewig
Date: Sep 24, 2020，Views：869
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
RT-LAMP assay
Authors: Viet Loan Dao Thi, Michael Knop and Simon Anders
Date: Sep 24, 2020，Views：886
Original protocol used in research article：
A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Science Translational Medicine, Aug 12, 2020; DOI: 10.1126/scitranslmed.abc7075
Untargeted metabolomics
Author: Carlos Guijas
Date: Sep 23, 2020，Views：923
Original protocol used in research article：
Metabolic adaptation to calorie restriction
Science Signaling, Sep 8, 2020; DOI: 10.1126/scisignal.abb2490
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