Protocols shared by authors via RaP service
Journal
CDR3 tree map analysis
Authors: Jian Han and Leonore A Herzenberg
Date: Oct 20, 2020，Views：940
Original protocol used in research article：
Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
eLife, Sep 30, 2015; DOI: 10.7554/eLife.09083
Cuticular hydrocarbon extractions and GC analysis
Authors: Cassondra L Vernier, Yehuda Ben-Shahar and Joshua Krupp
Date: Oct 20, 2020，Views：970
Original protocol used in research article：
The cuticular hydrocarbon profiles of honey bee workers develop via a socially-modulated innate process
eLife, Feb 5, 2019; DOI: 10.7554/eLife.41855
Zymography
Author: Trayambak Pathak
Date: Oct 20, 2020，Views：1015
Original protocol used in research article：
Dichotomous role of the human mitochondrial Na+/Ca2+/Li+ exchanger NCLX in colorectal cancer growth and metastasis
eLife, Sep 11, 2020; DOI: 10.7554/eLife.59686
On-chip sort, 10X library preparation, and RNA sequencing
Authors: Benjamin W Okaty, Susan M Dymecki and Nikita Sturrock
Date: Oct 19, 2020，Views：1024
Original protocol used in research article：
A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons
eLife, Jun 22, 2020; DOI: 10.7554/eLife.55523
Wireless transmitter and tetrode fabrication
Author: Haleh Fotowat
Date: Oct 19, 2020，Views：848
Original protocol used in research article：
Neural activity in a hippocampus-like region of the teleost pallium is associated with active sensing and navigation
eLife, Apr 3, 2019; DOI: 10.7554/eLife.44119
Antisense oligonucleotide application
Author: Jonathan R Roth
Date: Oct 19, 2020，Views：1047
Original protocol used in research article：
Alzheimer’s disease risk gene BIN1 induces Tau-dependent network hyperexcitability
eLife, Jul 13, 2020; DOI: 10.7554/eLife.57354
Construct design and 3D printing process
Author: Fiona E. Freeman
Date: Oct 19, 2020，Views：1045
Original protocol used in research article：
3D bioprinting spatiotemporally defined patterns of growth factors to tightly control tissue regeneration
Science Advances, Aug 14, 2020; DOI: 10.1126/sciadv.abb5093
CytofRUV vignette
Author: Marie Trussart
Date: Oct 19, 2020，Views：967
Original protocol used in research article：
Removing unwanted variation with CytofRUV to integrate multiple CyTOF datasets
eLife, Sep 7, 2020; DOI: 10.7554/eLife.59630
Skeletal stain followed by micro-computed tomography
Authors: Camilla S Teng, Robert E Maxson and J Gage Crump
Date: Oct 18, 2020，Views：905
Original protocol used in research article：
Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome
eLife, Oct 25, 2018; DOI: 10.7554/eLife.37024
Western blots
Author: Weilin Zhang
Date: Oct 15, 2020，Views：963
Original protocol used in research article：
Hypoxic mitophagy regulates mitochondrial quality and platelet activation and determines severity of I/R heart injury
eLife, Dec 20, 2016; DOI: 10.7554/eLife.21407
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