Protocols shared by authors via RaP service
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Live Cell Imaging of EGF Binding to A431 Cells
Authors: Dongmyung Oh, Joshua A Jadwin, Bruce J Mayer and Ji Yu
Date: Nov 2, 2020，Views：912
Original protocol used in research article：
Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases
eLife, Apr 13, 2016; DOI: 10.7554/eLife.11835
Isolation of synaptosomes and glutamate release assay
Author: Reinhard Jahn
Date: Nov 2, 2020，Views：917
Original protocol used in research article：
Analysis of protein phosphorylation in nerve terminal reveals extensive changes in active zone proteins upon exocytosis
eLife, Apr 26, 2016; DOI: 10.7554/eLife.14530
Western blotting
Author: Jonathan Houseley
Date: Nov 2, 2020，Views：992
Original protocol used in research article：
Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells
eLife, Oct 2, 2018; DOI: 10.7554/eLife.34081
Computation of evolutionary couplings
Author: Alexandre M J J Bonvin
Date: Nov 2, 2020，Views：1016
Original protocol used in research article：
Sequence co-evolution gives 3D contacts and structures of protein complexes
eLife, Sep 25, 2014; DOI: 10.7554/eLife.03430
Demonstration of the Stimulatory Effect of Lipopolysaccharide (LPS) on Egg Laying in Caenorhabditis elegans
Authors: Angela Ching-Yee Leung and Myeongwoo Lee
Date: Nov 2, 2020，Views：796
Original protocol used in research article：
Lipopolysaccharide stimulates egg laying in Caenorhabditis elegans
microPublication Biology, Sep 14, 2020; DOI: 10.17912/micropub.biology.000308
Generation of droplet-based artificial cells
Authors: Ye Guan, Shiyuan Wang and Qiong Yang
Date: Nov 2, 2020，Views：1042
Original protocol used in research article：
A robust and tunable mitotic oscillator in artificial cells
eLife, Apr 5, 2018; DOI: 10.7554/eLife.33549
Golgi-cox staining, dendritic spine analysis and neuronal morphology
Author: W Christopher Risher
Date: Nov 2, 2020，Views：1037
Original protocol used in research article：
Astrocytes refine cortical connectivity at dendritic spines
eLife, Dec 17, 2014; DOI: 10.7554/eLife.04047
In vitro efferocytosis
Authors: Arif Yurdagul Jr. and Ira Tabas
Date: Oct 31, 2020，Views：1037
Original protocol used in research article：
siRNA nanoparticles targeting CaMKIIγ in lesional macrophages improve atherosclerotic plaque stability in mice
Science Translational Medicine, Jul 21, 2020; DOI: 10.1126/scitranslmed.aay1063
Statistical analysis
Authors: Cassondra L. Vernier and Yehuda Ben-Shahar
Date: Oct 30, 2020，Views：854
Original protocol used in research article：
The gut microbiome defines social group membership in honey bee colonies
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3431
Polysome and Puromycin immunoprecipitation
Authors: Benjamin D Hobson and Peter A Sims
Date: Oct 30, 2020，Views：1632
Original protocol used in research article：
Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048
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