List of antibodies used for cell sorting

List of antibodies used for cell sorting:
 

CD194/CCR4 [L291H4] PE (Biolegend, cat#359411); CD11c [Bu15] Brilliant Violet 421 (Biolegend, cat#337225); CD123 [6H6] FITC (Biolegend, cat#306014); CD127 [A019D5] Brilliant Violet 785 (Biolegend, cat#351329); CD14 [HCD14] PE (Biolegend, cat#325606); CD14 [HCD14] FITC (Biolegend, cat#325603); CD15 [MMA] eFluor 450 (eBioscience, cat#48- 0158-41); CD16 [3G8] Brilliant Violet 711 (Biolegend, cat#302044); CD161 [HP-3G10] Brilliant Violet 421 (Biolegend, cat#339913); CD19 [HIB19] PE/Cy7 (Biolegend, cat#302216); CD193/CCR3 [5E8-G9-B4] APC (eBioscience, cat#17-1939-42); CD20 [2H7] Brilliant Violet 650 (Biolegend, cat#302336); CD203c [NP4D6] PE (eBioscience, cat#12-2039-41); CD25 [M- A251] Brilliant Violet 421 (Biolegend, cat#356113); CD27 [L128] Brilliant Violet 650 (BD biosciences, cat#563228); CD3 [HIT3a] Alexa Fluor 700 (Biolegend, cat#300324); CD3 [UCHT1] BD Horizon V450 (BD biosciences, cat#560366); CD3 [UCHT1] APC/Fire 750 (Biolegend, cat#300469); CD38 [HB-7] FITC (Biolegend, cat#356609); CD38 [HIT2] PE/Cy5 (eBioscience, cat#15-0389-42); CD4 [RPA-T4] PE/Cy5 (Biolegend, cat#300510); CD45 [HI30] BUV395 (BD biosciences, cat#563792); CD45RA [HI100] Brilliant Violet 785 (Biolegend, cat#304140); CD56 [5.1H11] APC (Biolegend, cat#362503); CD56 [NCAM16.2] Brilliant Violet 421 (BD biosciences, cat#562752); CD8a [SK1] Alexa Fluor 488 (Biolegend, cat#344716); HLA-Dr [L243] Brilliant Violet 605 (Biolegend, cat#307640); TCR V⍺7.2 [3C10] PE (Biolegend, cat#351705); 𝛾ẟTCR [B1.1] PerCP-eFluor 710 (eBioscience, cat#46-9959-41);

Gating scheme of isolations of cells from PBMC. Arrows indicate sequential gating procedure starting from FSC/SSC plot distinguishing monocytes and lymphocytes. Figure shows one representative sample and multiple Flow cytometry panels.
 

Gating scheme of isolations of cells from whole blood. Arrows indicate sequential gating procedure starting from FSC/SSC plot. Figure shows one representative sample.

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