Protocols shared by authors via RaP service
Journal
Cell cluster annotation, trajectory inference, and signature enrichment
Authors: Ekaterini Maria Lyras and Alexander Mildner
Date: Apr 5, 2023，Views：307
Original protocol used in research article：
Tongue immune compartment analysis reveals spatial macrophage heterogeneity
eLife, ; DOI: 10.7554/eLife.77490
Expression and purification of CR3022 Fab and IgG
Authors: Meng Yuan, Nicholas C. Wu, Wenli Yu and Ian A. Wilson
Date: Apr 4, 2023，Views：77
Original protocol used in research article：
A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV
Science, May 7, 2020; DOI: 10.1126/science.abb7269
Preparation of odorant and buffer solutions
Authors: Albert Lin, Cengiz Pehlevan, Mei Zhen and Aravinthan D.T. Samuel
Date: Apr 4, 2023，Views：325
Original protocol used in research article：
Functional imaging and quantification of multineuronal olfactory responses in C. elegans
Science Advances, Mar 1, 2023; DOI: 10.1126/sciadv.ade1249
Experimental model and subject details
Author: Lucas Pozzo-Miller
Date: Apr 4, 2023，Views：55
Original protocol used in research article：
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory
eLife, May 21, 2019; DOI: 10.7554/eLife.44182
Detection of mitochondrial ROS levels using flow cytometry
Authors: Xiaowei Zheng and Sergiu-Bogdan Catrina
Date: Apr 3, 2023，Views：443
Original protocol used in research article：
Repression of hypoxia-inducible factor-1 contributes to increased mitochondrial reactive oxygen species production in diabetes
eLife, Feb 15, 2022; DOI: 10.7554/eLife.70714
Generation of rescued mouse ES cell lines expressing EGFP-tagged Suv39h proteins
Authors: Thomas Jenuwein and Kalina Malgorzata Swist-Rosowska
Date: Mar 31, 2023，Views：287
Original protocol used in research article：
Major satellite repeat RNA stabilize heterochromatin retention of Suv39h enzymes by RNA-nucleosome association and RNA:DNA hybrid formation
eLife, Aug 1, 2017; DOI: 10.7554/eLife.25293
Reagents and infection with SARS-CoV-2
Authors: Gustavo Gastão Davanzo and Marcelo Hill
Date: Mar 27, 2023，Views：265
Original protocol used in research article：
PD-1/PD-L1 blockade abrogates a dysfunctional innate-adaptive immune axis in critical β-coronavirus disease
Science Advances, Sep 21, 2022; DOI: 10.1126/sciadv.abn6545
Immunofluorescence
Author: Sarah E Siegrist
Date: Mar 27, 2023，Views：299
Original protocol used in research article：
Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila
eLife, Aug 9, 2017; DOI: 10.7554/eLife.26343
Widefield and two-photon calcium imaging
Author: Matthew McGill
Date: Mar 27, 2023，Views：299
Original protocol used in research article：
Neural signatures of auditory hypersensitivity following acoustic trauma
eLife, Sep 16, 2022; DOI: 10.7554/eLife.80015
Adapted ATAC-seq protocol from Monahan et al eLife 2017
Author: Kevin Gunther Monahan
Date: Mar 27, 2023，Views：321
Original protocol used in research article：
Cooperative interactions enable singular olfactory receptor expression in mouse olfactory neurons
eLife, Sep 21, 2017; DOI: 10.7554/eLife.28620
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.